Immobilization of Alkaline Protease From Bacillus brevis Using Ca-Alginate Entrapment Strategy for Improved Catalytic Stability, Silver Recovery, and Dehairing Potentialities

The waste X-ray/ photographic films contain 1.5 - 2 % (w/w) black metallic silver which isrecovered and reused. Around 18-20% of the world's silver needs are supplied by recyclingphotographic waste. Since silver is linked to gelatin in the emulsion layer, it is possible to break thesame and release the silver using proteolytic enzymes. Alkaline protease from Conidioboluscoronatus was investigated for enzymatic hydrolysis of gelatin from waste X-ray films. At the endof the treatment, gelatin layer was completely removed leaving the polyester film clean and silverwas recovered in the hydrolysate, both of which can be reused. Various parameters such as pH,temperature, enzyme concentration, time etc on silver removal from the film were studied. Gelatinhydrolysis was monitored by measuring increase in turbidity in the hydrolysate, which wasaccompanied by release of protein and hydroxyproline. Gelatin layer was stripped completelywithin 6 min with 1.35 U ml-1 of protease at 40°C, pH 10. Rate of gelatin hydrolysis increased withincreased in protease concentration. The enzyme could be effectively reused for four cycles ofgelatin hydrolysis. Silver in hydolysate was around 3.87% (w/w) based on total weight of sludge.Key words: Silver recovery; X-ray films; gelatin hydrolysis; alkaline protease; Conidiobolus coronatusDOI: 10.3126/kuset.v6i1.3311 Kathmandu University Journal of Science, Engineering and Technology Vol.6(1) 2010, pp60-69

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Production of alkaline protease by Teredinobacter turnirae cells immobilized in Ca-alginate beads

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2003.000-1012 ◽

2003 ◽

Vol 2 (3) ◽

pp. 60-65 ◽

Cited By ~ 25

Author(s):

Beshay Usama

Keyword(s):

Alkaline Protease ◽

Alginate Beads ◽

Teredinobacter Turnirae ◽

Ca Alginate

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Molecular Characterization of Alkaline Protease Gene Isolated from Aeromonas hydrophila strain AH10

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v8i2.29583 ◽

2020 ◽

Vol 8 (2) ◽

pp. 154-160

Author(s):

N.G. Ramesh Babu ◽

Anupa Mary Aji

Keyword(s):

Aeromonas Hydrophila ◽

Alkaline Protease ◽

Recovery Process ◽

Protease Gene ◽

Gene Encoding ◽

Silver Recovery ◽

Ph Optimum ◽

Protease Enzyme ◽

Alkaline Protease Gene

Alkaline protease enzymes are enzymes which can catalyze the process of proteolysis between the pH ranges from 8 to 12. Extracellular alkaline proteases are used as additives in detergent powders. In the present study, source of the organism was from a detergent contaminated area. The study has been carried out in Aeromonas hydrophila AH10 strain that produces protease enzyme with an alkaline pH optimum. The organism was a gram-negative rod with a protease enzyme activity of 0.385 ml/min. purification of the protease enzyme from the bacteria was carried out. This protease is suitable for use in alkaline detergent powders as well as in silver recovery process. The Aeromonas hydrophila strain AH10 gene encoding this high-alkaline protease was cloned and characterized. Int. J. Appl. Sci. Biotechnol. Vol 8(2): 154-160

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