<p>Phylogenetic and Maturity Analyses of Sixty Soybean<br />Genotypes Used for DNA Marker Development of Early<br />Maturity Quantitative Trait Loci in Soybean. I Made<br />Tasma, Dani Satyawan, Ahmad Warsun, Muhamad<br />Yunus, and Budi Santosa. The Indonesian soybean<br />productivity is still very low with the national average of 1.3<br />t/ha. One means to improve national soybean productivity is<br />by manipulating harvest index by cultivating very early<br />maturing soybean cultivars. Development of early maturing<br />soybean cultivars can be expedited by using marker-aided<br />selection. The objective of this study was to select parental<br />lines having contrasted maturity traits and selected parents<br />must be genetically distance. The parents then were used to<br />develop F2 populations for detecting early maturity QTL in<br />soybean. Maturity tests of 60 soybean genotypes were<br />conducted at two locations, Cikeumeuh (Bogor) and Pacet<br />(Cianjur) using a randomized block design with three<br />replications. Genomic DNA of the 60 genotypes were<br />analyzed using 18 SSR markers and genetic relationship was<br />constructed using the Unweighted Pair-Group Method<br />Arithmatic through Numerical Taxonomy and Multivariate<br />System program version 2.1-pc. Results showed that the 60<br />genotypes demonstrated normal distribution in both<br />locations for days to R1 (32-48d), days to R3 (35-55d), days to<br />R7 (75-92d), and days to R8 (78-99d). Four early maturing<br />genotypes and three late genotypes were obtained. Total<br />SSR alleles observed were 237 with average allele per locus<br />of 12.6 (3-29), and average PIC value of 0.78 (0.55-0.89).<br />Genetic similarity among genotypes ranges from 74.8-95%.<br />At similarity level 77% divided the genotypes into six clusters<br />(the four selected early maturing genotypes located in<br />clusters III and IV, while the three late genotypes located in<br />cluster II). Based on maturity data, pubescent color, and<br />phygenetic analysis seven parents were selected (four early<br />maturing genotypes B1430, B2973, B3611, B4433 and three<br />late genotypes B1635, B1658, and B3570). Twelve F2<br />populations were developed with the aid of SSR markers<br />Satt300 dan Satt516. Two of the populations will be used to<br />develop DNA markers for earliness in soybean.</p>