scholarly journals Direct genotyping of single pollen grains of a self-compatible mutant of Japanese pear (Pyrus pyrifolia) revealed inheritance of a duplicated chromosomal segment containing a second S-haplotype

Euphytica ◽  
2014 ◽  
Vol 200 (2) ◽  
pp. 297-304 ◽  
Author(s):  
Nobuko Mase ◽  
Yutaka Sawamura ◽  
Toshiya Yamamoto ◽  
Norio Takada ◽  
Sogo Nishio ◽  
...  
2016 ◽  
Vol 26 (2) ◽  
pp. 185-190
Author(s):  
Daisuke Sakamoto ◽  
Shinnosuke Kusaba ◽  
Yuri Nakamura

The effects on fruit set of adding forchlorfenuron (CPPU) to media in which pollen grains were suspended and used for spray pollination was investigated in ‘Hosui’ japanese pear (Pyrus pyrifolia). The percentages of fruit set after spray pollination were lower than those after hand pollination; however, media with 2 or 10 mg·L−1 CPPU used for spray pollination resulted in greater percentages of fruit set than media without CPPU. The media with CPPU also resulted in higher levels of pollen germination compared with the medium without CPPU; however, the number of seeds per fruit with CPPU treatment tended to be almost the same or less than for fruit without CPPU treatment. From these results, the higher levels of fruit set by CPPU might not be due to the high germination rate of the pollen grains but due to the induction of partial parthenocarpic properties. The medium with 10 mg·L−1 CPPU gave the highest levels of fruit set, but the fruit had abnormally thickened and enlarged calyxes, and the fruit shape tended to be deformed at ≈21 days after flowering. In addition, all of the harvested fruit were calyx-perpetual fruit in which the calyx remains until harvest time. Fruit treated with 2 mg·L−1 CPPU also yielded calyx-perpetual fruit with high frequency, but the fruit had normal calyxes and the fruit shape tended to be more normal. Thus, we conducted experiments focusing on the medium containing 2 mg·L−1 CPPU. Although the results were variable from year to year, more than 70% of hand-pollinated flowers set fruit during the 3-year study; this level of fruit production is sufficient for practical use. Thus, spray pollination using a medium with 2 mg·L−1 CPPU is an acceptable method for pollinating ‘Hosui’ japanese pear.


2013 ◽  
Vol 33 (1) ◽  
pp. 117-128 ◽  
Author(s):  
Nobuko Mase ◽  
Yutaka Sawamura ◽  
Toshiya Yamamoto ◽  
Norio Takada ◽  
Sogo Nishio ◽  
...  

2002 ◽  
Vol 19 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Carlos CASTILLO ◽  
Takeshi TAKASAKI ◽  
Toshihiro SAITO ◽  
Shigemi NORIOKA ◽  
Tetsu NAKANISHI

Author(s):  

Abstract A new distribution map is provided for Botryosphaeria berengeriana f.sp. piricola (Nose) Koganezawa & Sakuma. Hosts: Japanese pear (Pyrus pyrifolia), also European pear (P. communis) and apple (Malus pumila). Information is given on the geographical distribution in China, Anhui, Fujian, Guangxi, Hebei, Henan, Hubei, Jiangsu, Jiangxi, Jilin, Liaoning, Shandong, Shanxi, Sichuan, Zhejiang, Japan, Honshu, Shikoku, Korea Dem. People's Republic, Korea Republic, Taiwan.


2020 ◽  
Author(s):  
Hideo Ishii ◽  
Kumiko Nishimura ◽  
Kenji Tanabe ◽  
Yuichi Yamaoka

Scab, caused by Venturia nashicola is one of the most serious diseases of Asian pears including Japanese pear (Pyrus pyrifolia var. culta) and Chinese pears (P. bretschneideri and P. ussuriensis). Breeding of scab-resistant pear cultivars is essential to minimize the use of fungicides and the risk of fungicide resistance developing in the pathogen. A survey of pathogenic specialization in V. nashicola is needed to ensure durable scab resistance in cultivated pears. Race 1, 2, and 3 isolates of V. nashicola, each differing in pathogenicity to Japanese pear cv. Kousui and Asian pear strain Mamenashi 12, have been reported from Japan. In the present study, isolates collected from scabbed pears in China and Taiwan were classified as V. nashicola based on conidial size and mating ability. However, various isolates were found to have pathogenicity distinct from races 1, 2, and 3 in tests on seven differential host genotypes: Kousui; Mamenashi 12; Chinese pear cvs. Jingbaili, Yali, Linyuli, Nanguoli; and Taiwanese pear cv. Hengshanli. The new races were designated as races 4 to 7. Progenies characteristic of race 3 isolates were produced in a cross between race 1 and race 2 isolates, suggesting the possible role of sexual recombination in the emergence of novel races. Japanese pear cv. Kinchaku and cv. Xiangli of P. sinkiangensis (Korla fragrant pear grown in China) didn’t show visible symptoms after inoculation with any of the seven races. The broad scab resistance in Kinchaku and Xiangli makes them a promising genetic resource for resistance breeding programs.


2005 ◽  
Vol 74 (3) ◽  
pp. 255-257 ◽  
Author(s):  
Haruyuki Kuroda ◽  
Toshihiko Sugiura ◽  
Hiroyoshi Sugiura

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