Approaches to rapid screening of pharmaceutical xenobiotic effects on microalgae via monitoring of photosynthetic apparatus condition

2022 ◽  
Author(s):  
Alexei E. Solovchenko ◽  
Svetlana G. Vasilieva ◽  
Petr Zaitsev ◽  
Alexandr A. Lukyanov ◽  
Elena V. Skripnikova ◽  
...  
Keyword(s):  
Photosynthetic Apparatus ◽  
Rapid Screening

scholarly journals Spectral method of evaluation of photosynthetic apparatus thermal resistance

2016 ◽  
Vol 14 (2) ◽  
pp. 239-244
Author(s):  
V. V. Shevchenko ◽  
O. Yu. Bondarenko
Keyword(s):  
Thermal Stability ◽  
Winter Wheat ◽  
Heat Resistance ◽  
Spectral Parameter ◽  
Photosynthetic Apparatus ◽  
Rapid Screening ◽  
Short Term ◽  
Wheat Varieties ◽  
Method Of Evaluation ◽  
Relationship Of

Aim. The aim of this work is to evaluate the possibilities of application of the spectral parameter - the relationship of the absorption values at wavelengths of 680 and 850 nm (k = A680 / A850) for testing heat resistance of plants to change the size of chloroplasts at short-term heating.  Methods. The short-term (5 minutes) heating in the range 25-45oC was carried out for chloroplasts isolated from two-week-old pea, corn and 4 winter wheat varieties differing in heat resistance. From the absorption spectra of chloroplasts was calculated changes of parameter k. Results. All heated chloroplasts showed changes in the spectral parameter k. Particularly strong and stable changes observed during heating at 40-45 ° C. In terms of changes in the spectral parameter plants are ranged in the following order - corn, pea, wheat. Conclusions. The intensity of the k changes coincided with the preliminary estimation of thermal stability for corn and different varieties of winter wheat. It is recommended to use the spectral parameter k for the rapid screening of varieties on the thermal stability of the photosynthetic apparatus within the same species.Key words: chloroplasts of different species of plants, the photosynthetic apparatus, short-term heating, evaluation of the thermal stability.

Macroorganisation and flexibility of thylakoid membranes

2019 ◽  
Vol 476 (20) ◽  
pp. 2981-3018 ◽  
Author(s):  
Petar H. Lambrev ◽  
Parveen Akhtar
Keyword(s):  
Light Harvesting ◽  
Current Knowledge ◽  
Three Dimensional ◽  
Photosynthetic Apparatus ◽  
Dynamic Responses ◽  
State Transitions ◽  
Photochemical Quenching ◽  
Light Harvesting Complex ◽  
Complex Ii ◽  
Light Harvesting Complex Ii

Abstract The light reactions of photosynthesis are hosted and regulated by the chloroplast thylakoid membrane (TM) — the central structural component of the photosynthetic apparatus of plants and algae. The two-dimensional and three-dimensional arrangement of the lipid–protein assemblies, aka macroorganisation, and its dynamic responses to the fluctuating physiological environment, aka flexibility, are the subject of this review. An emphasis is given on the information obtainable by spectroscopic approaches, especially circular dichroism (CD). We briefly summarise the current knowledge of the composition and three-dimensional architecture of the granal TMs in plants and the supramolecular organisation of Photosystem II and light-harvesting complex II therein. We next acquaint the non-specialist reader with the fundamentals of CD spectroscopy, recent advances such as anisotropic CD, and applications for studying the structure and macroorganisation of photosynthetic complexes and membranes. Special attention is given to the structural and functional flexibility of light-harvesting complex II in vitro as revealed by CD and fluorescence spectroscopy. We give an account of the dynamic changes in membrane macroorganisation associated with the light-adaptation of the photosynthetic apparatus and the regulation of the excitation energy flow by state transitions and non-photochemical quenching.

Rapid screening test for porphyrinuria

1970 ◽  
Vol 102 (2) ◽  
pp. 237-237
Author(s):  
R. M. McDonald
Keyword(s):  
Screening Test ◽  
Rapid Screening ◽  
Rapid Screening Test

Rapid screening and targeted isolation of antimalarial hits using β-Hematin assay

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
K Ndjoko Ioset ◽  
S Vargas ◽  
AE Hay ◽  
JR Ioset ◽  
K Hostettmann
Keyword(s):  
Rapid Screening

Rapid Screening Methods for Bleeding Disorders. A Three Year Survey

1964 ◽  
Vol 11 (02) ◽  
pp. 506-512 ◽  
Author(s):  
V. A Lovric ◽  
J Margolis
Keyword(s):  
Laboratory Tests ◽  
Capillary Blood ◽  
Screening Tests ◽  
Rapid Screening ◽  
Screening Methods ◽  
Bleeding Disorders ◽  
Routine Laboratory ◽  
Clotting Time ◽  
Kaolin Clotting Time ◽  
In Infants And Children

SummaryAn adaptation of “kaolin clotting time” and prothrombin time for use on haemolysed capillary blood provided simple and sensitive screening tests suitable for use in infants and children. A survey of three year’s experience shows that these are reliable routine laboratory tests for detection of latent coagulation disorders.

Pigments of photosynthetic apparatus in Spirulina platensis (Nordst.) Geitl.

2000 ◽  
Vol 2 (2) ◽  
pp. 91-95
Author(s):  
L. A. Denchikova ◽  
Yu. V. Skutaru ◽  
V. F. Rudik
Keyword(s):  
Spirulina Platensis ◽  
Photosynthetic Apparatus
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