scholarly journals Segmental isotopic labeling of a 140 kDa dimeric multi-domain protein CheA from Escherichia coli by expressed protein ligation and protein trans-splicing

2012 ◽  
Vol 53 (3) ◽  
pp. 191-207 ◽  
Author(s):  
Yuichi Minato ◽  
Takumi Ueda ◽  
Asako Machiyama ◽  
Ichio Shimada ◽  
Hideo Iwaï
Keyword(s):  
Escherichia Coli ◽  
Isotopic Labeling ◽  
Expressed Protein Ligation ◽  
Protein Ligation ◽  
Trans Splicing ◽  
Domain Protein ◽  
Segmental Isotopic Labeling

scholarly journals Segmental isotopic labeling of the Hsp70 molecular chaperone DnaK using expressed protein ligation

Biopolymers ◽  
2010 ◽  
Vol 94 (6) ◽  
pp. 742-752 ◽  
Author(s):  
Eugenia M. Clerico ◽  
Anastasia Zhuravleva ◽  
Robert G. Smock ◽  
Lila M. Gierasch
Keyword(s):  
Molecular Chaperone ◽  
Isotopic Labeling ◽  
Expressed Protein Ligation ◽  
Protein Ligation ◽  
Chaperone Dnak ◽  
Segmental Isotopic Labeling

scholarly journals Segmental isotopic labeling by asparaginyl endopeptidase-mediated protein ligation

2018 ◽  
Vol 71 (4) ◽  
pp. 225-235 ◽  
Author(s):  
Kornelia M. Mikula ◽  
Luisa Krumwiede ◽  
Andreas Plückthun ◽  
Hideo Iwaï
Keyword(s):  
Isotopic Labeling ◽  
Protein Ligation ◽  
Asparaginyl Endopeptidase ◽  
Segmental Isotopic Labeling

Segmental isotopic labeling of multi-domain and fusion proteins by protein trans-splicing in vivo and in vitro

2010 ◽  
Vol 5 (3) ◽  
pp. 574-587 ◽  
Author(s):  
Mikko Muona ◽  
A Sesilja Aranko ◽  
Vytas Raulinaitis ◽  
Hideo Iwaï
Keyword(s):  
Fusion Proteins ◽  
Isotopic Labeling ◽  
Trans Splicing ◽  
Segmental Isotopic Labeling

Semisynthetic head-to-tail cyclized peptides obtained by combining protein trans-splicing and intramolecular expressed protein ligation

2021 ◽  
Author(s):  
Shubhendu Palei ◽  
Henning D. Mootz
Keyword(s):  
Expressed Protein Ligation ◽  
Peptide Bonds ◽  
Protein Ligation ◽  
Native Peptide ◽  
Split Intein ◽  
Trans Splicing ◽  
Macrocyclic Peptides

A dual-intein approach for the preparation of head-to-tail macrocyclic peptides is reported, where synthetic and genetically encoded fragments are ligated by two native peptide bonds. A split intein ligates the...

Sign in / Sign up

Export Citation Format

Share Document