Characterization of disease resistance gene candidates of the nucleotide binding site (NBS) type from banana and correlation of a transcriptional polymorphism with resistance to Fusarium oxysporum f.sp. cubense race 4

The Cre3 gene confers a high level of resistance to the root endoparasitic nematode Heterodera avenae in wheat. A DNA marker cosegregating with H. avenae resistance was used as an entry point for map-based cloning of a disease resistance gene family at the Cre3 locus. Two related gene sequences have been analysed at the Cre3 locus. One, identified as a cDNA clone, encodes a polypeptide with a nucleotide binding site (NBS) and a leucine-rich region; this member of the disease resistance gene family is expressed in roots. A second Cre3 gene sequence, cloned as genomic DNA, appears to be a pseudogene, with a frame shift caused by a deletion event. These two genes, related to members of the cytoplasmic NBS – leucine rich repeat class of plant disease resistance genes were physically mapped to the distal 0.06 fragment of the long arm of wheat chromosome 2D and cosegregated with nematode resistance.Key words: cereal cyst nematode, disease resistance genes, nucleotide-binding site, leucine-rich repeat.

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A superfamily of disease resistance gene analogs is located on all homoeologous chromosome groups of wheat (Triticum aestivum)

Genome ◽

10.1139/g98-083 ◽

1998 ◽

Vol 41 (6) ◽

pp. 782-788 ◽

Cited By ~ 11

Author(s):

W Spielmeyer ◽

M Robertson ◽

N Collins ◽

D Leister ◽

P Schulze-Lefert ◽

...

Keyword(s):

Disease Resistance ◽

Binding Site ◽

Resistance Gene ◽

Resistance Genes ◽

Nucleotide Binding ◽

Nucleotide Binding Site ◽

Resistance Gene Analogs ◽

Leucine Rich Repeat ◽

Homoeologous Chromosome ◽

Entry Points

In this study, resistance gene analogs (RGAs) which were isolated from monocot crop species (wheat, barley, maize and rice) and contained conserved sequence motifs found within the nucleotide binding site - leucine rich repeat (NBS-LRR) class of resistance genes, were used to assess their distribution in the wheat genome. The RGAs showed 30-70% amino acid identity to a previously isolated monocot NBS-LRR sequence from the Cre3 locus for cereal cyst nematode (CCN) resistance in wheat. We used the RGAs as probes to identify and map loci in wheat using recombinant inbred lines of an international Triticeae mapping family. RGA loci mapped across all seven homoeologous chromosome groups of wheat. This study demonstrated that the RGA mapping approach provides potential entry points toward identifying resistance gene candidates in wheat.Key words: wheat, disease resistance genes, nucleotide binding site, leucine rich repeat, resistance gene analogs.

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Characterization of resistance gene analogs with a nucleotide binding site isolated from a triploid white poplar

Plant Biology ◽

10.1111/j.1438-8677.2008.00029.x ◽

2008 ◽

Vol 10 (3) ◽

pp. 310-322 ◽

Cited By ~ 21

Author(s):

Q. Zhang ◽

Z.-Y. Zhang ◽

S.-Z. Lin ◽

H.-Q. Zheng ◽

Y.-Z. Lin ◽

...

Keyword(s):

Binding Site ◽

Resistance Gene ◽

Nucleotide Binding ◽

Nucleotide Binding Site ◽

Resistance Gene Analogs ◽

White Poplar

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Deletion of a Disease Resistance Nucleotide-Binding-Site Leucine-Rich- Repeat-like Sequence Is Associated With the Loss of the Phytophthora Resistance Gene Rps4 in Soybean

Genetics ◽

10.1534/genetics.104.032037 ◽

2004 ◽

Vol 168 (4) ◽

pp. 2157-2167 ◽

Cited By ~ 70

Author(s):

Devinder Sandhu ◽

Hongyu Gao ◽

Silvia Cianzio ◽

Madan K. Bhattacharyya

Keyword(s):

Disease Resistance ◽

Binding Site ◽

Resistance Gene ◽

Nucleotide Binding ◽

Nucleotide Binding Site ◽

Leucine Rich Repeat ◽

Phytophthora Resistance

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Candidate disease resistance genes in sunflower cloned using conserved nucleotide-binding site motifs: Genetic mapping and linkage to the downy mildew resistance gene Pl1

Genome ◽

10.1139/g00-110 ◽

2001 ◽

Vol 44 (2) ◽

pp. 205-212 ◽

Cited By ~ 29

Author(s):

Melaku Ayele Gedil ◽

Mary B Slabaugh ◽

Simon Berry ◽

Richard Johnson ◽

Richard Michelmore ◽

...

Keyword(s):

Disease Resistance ◽

Binding Site ◽

Downy Mildew ◽

Resistance Gene ◽

Nucleotide Binding ◽

Nucleotide Binding Site ◽

Sequence Motifs ◽

Caps Marker ◽

Race 1 ◽

Dna Sequence Motifs

Disease resistance gene candidates (RGCs) belonging to the nucleotide-binding site (NBS) superfamily have been cloned from numerous crop plants using highly conserved DNA sequence motifs. The aims of this research were to (i) isolate genomic DNA clones for RGCs in cultivated sunflower (Helianthus annuus L.) and (ii) map RGC markers and Pl1, a gene for resistance to downy mildew (Plasmopara halstedii (Farl.) Berl. & de Toni) race 1. Degenerate oligonucleotide primers targeted to conserved NBS DNA sequence motifs were used to amplify RGC fragments from sunflower genomic DNA. PCR products were cloned, sequenced, and assigned to 11 groups. RFLP analyses mapped six RGC loci to three linkage groups. One of the RGCs (Ha-4W2) was linked to Pl1, a downy mildew resistance gene. A cleaved amplified polymorphic sequence (CAPS) marker was developed for Ha-4W2 using gene-specific oligonucleotide primers. Downy mildew susceptible lines (HA89 and HA372) lacked a 276-bp Tsp509I restriction fragment that was present in downy mildew resistant lines (HA370, 335, 336, 337, 338, and 339). HA370 × HA372 F2 progeny were genotyped for the Ha-4W2 CAPS marker and phenotyped for resistance to downy mildew race 1. The CAPS marker was linked to but did not completely cosegregate with Pl1 on linkage group 8. Ha-4W2 was found to comprise a gene family with at least five members. Although genetic markers for Ha-4W2 have utility for marker-assisted selection, the RGC detected by the CAPS marker has been ruled out as a candidate gene for Pl1. Three of the RGC probes were monomorphic between HA370 and HA372 and still need to be mapped and screened for linkage to disease resistance loci.Key words: Helianthus, sunflower, downy mildew, Plasmopara, nucleotide-binding site.

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