scholarly journals TILLING, high-resolution melting (HRM), and next-generation sequencing (NGS) techniques in plant mutation breeding

2017 ◽  
Vol 37 (3) ◽  
Author(s):  
Sima Taheri ◽  
Thohirah Lee Abdullah ◽  
Shri Mohan Jain ◽  
Mahbod Sahebi ◽  
Parisa Azizi
Keyword(s):  
Next Generation Sequencing ◽  
High Resolution ◽  
High Resolution Melting ◽  
Mutation Breeding ◽  
Next Generation ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing

scholarly journals Microsatellite marker development for the tetraploid Veronica aragonensis (Plantaginaceae) using next-generation sequencing and high-resolution melting analyses

2018 ◽  
Vol 6 (5) ◽  
pp. e01154
Author(s):  
Nélida Padilla-García ◽  
Teresa Malvar-Ferreras ◽  
Josie Lambourdière ◽  
M. Montserrat Martínez-Ortega ◽  
Nathalie Machon
Keyword(s):  
Next Generation Sequencing ◽  
High Resolution ◽  
Microsatellite Marker ◽  
High Resolution Melting ◽  
Marker Development ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals Comparison of a High-Resolution Melting Assay to Next-Generation Sequencing for Analysis of HIV Diversity

2012 ◽  
Vol 50 (9) ◽  
pp. 3054-3059 ◽  
Author(s):  
M. M. Cousins ◽  
S.-S. Ou ◽  
M. J. Wawer ◽  
S. Munshaw ◽  
D. Swan ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
High Resolution ◽  
High Resolution Melting ◽  
Next Generation ◽  
Generation Sequencing ◽  
Hiv Diversity

scholarly journals Determination ofRETSequence Variation in an MEN2 Unaffected Cohort Using Multiple-Sample Pooling and Next-Generation Sequencing

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
R. L. Margraf ◽  
J. D. Durtschi ◽  
J. E. Stephens ◽  
M. Perez ◽  
K. V. Voelkerding
Keyword(s):  
Next Generation Sequencing ◽  
Sequence Variation ◽  
High Resolution Melting ◽  
Next Generation ◽  
Sample Pooling ◽  
Melting Analysis ◽  
Multiple Sample ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing

Multisample, nonindexed pooling combined with next-generation sequencing (NGS) was used to discoverRETproto-oncogene sequence variation within a cohort known to be unaffected by multiple endocrine neoplasia type 2 (MEN2). DNA samples (113 Caucasians, 23 persons of other ethnicities) were amplified forRETintron 9 to intron 16 and then divided into 5 pools of <30 samples each before library prep and NGS. Two controls were included in this study, a single sample and a pool of 50 samples that had been previously sequenced by the same NGS methods. All 59 variants previously detected in the 50-pool control were present. Of the 61 variants detected in the unaffected cohort, 20 variants were novel changes. Several variants were validated by high-resolution melting analysis and Sanger sequencing, and their allelic frequencies correlated well with those determined by NGS. The results from this unaffected cohort will be added to theRETMEN2 database.

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