Identification of quantitative trait loci for Fusarium head blight (FHB) resistance in the cross between wheat landrace N553 and elite cultivar Yangmai 13

Fusarium head blight (FHB), mainly caused by Fusarium graminearum, is a destructive disease in wheat. A population consisting of 229 F2 and F2:3 plants derived from the cross PI 672538 × L661 was used to evaluate the reactions to FHB. The FHB resistance data distribution in the F2 population indicates that some quantitative trait loci (QTLs) were controlling the FHB resistance in PI 672538. We further detected two major QTLs (Qfhs-2B, Qfhs-3B) from analysis of the resistance data and the PCR-amplified results using WinQTLCart 2.5 software. Qfhs-2B, flanked by Xbarc55-2B and Xbarc1155-2B, explained more than 11.6% of the phenotypic variation of the percentage of diseased spikelets (PDS), and Qfhs-3B, flanked by Xwmc54-3B and Xgwm566-3B, explained more than 10% of the PDS phenotypic variation in the F2:3 population. In addition, Qfhs-3B was different from Fhb1 in terms of the pedigree, inheritance, resistance response, chromosomal location, and marker diagnosis. We also detected QTLs for other disease resistance indices, including the percentage of damaged kernels and 1,000-grain weight, in similar chromosomal regions. Therefore, the FHB resistance of PI 672538 was mainly controlled by two major QTLs, mapped on 2B (FhbL693a) and 3B (FhbL693b). PI 672538 could be a useful germplasm for improving wheat FHB resistance.

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Quantitative trait loci for resistance to Fusarium head blight in the Chinese wheat landrace Huangfangzhu

Euphytica ◽

10.1007/s10681-012-0631-2 ◽

2012 ◽

Vol 185 (1) ◽

pp. 93-102 ◽

Cited By ~ 18

Author(s):

Tao Li ◽

Guihua Bai ◽

Shuangye Wu ◽

Shiliang Gu

Keyword(s):

Quantitative Trait Loci ◽

Fusarium Head Blight ◽

Quantitative Trait ◽

Head Blight ◽

Wheat Landrace ◽

Trait Loci ◽

Chinese Wheat

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Identification of Functional Genic Components of Major Fusarium Head Blight Resistance Quantitative Trait Loci in Wheat Cultivar Sumai 3

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-10-12-0235-r ◽

2013 ◽

Vol 26 (4) ◽

pp. 442-450 ◽

Cited By ~ 27

Author(s):

Yongbin Zhuang ◽

Aravind Gala ◽

Yang Yen

Keyword(s):

Quantitative Trait Loci ◽

Fusarium Head Blight ◽

Quantitative Trait ◽

Defense Mechanism ◽

Fusarium Head Blight Resistance ◽

Head Blight ◽

Bulk Analysis ◽

Trait Loci ◽

Fhb Resistance ◽

Sumai 3

Fusarium head blight (FHB) is a devastating disease worldwide, affecting wheat and other small grains. To identify key wheat genes involved in FHB pathogenesis, 406 FHB-related wheat expressed sequence tags functionally identified in Sumai 3 were investigated for their association with FHB-resistance quantitative trait loci (QTL) Fhb1 and Fhb_6BL in 2010 and 2011. A total of 47 candidate genes were identified by bulk analysis, near-isogenic screening and expression QTL mapping, and were finally mapped to their carrier chromosomes with Chinese Spring nulli-tetra deficiency lines. One gene, designated WFhb1_c1 (wheat Fhb1 candidate gene 1), was both functionally associated with and physically located within Fhb1 and was found to be weakly similar (E = 5e+0) to an Arabidopsis gene encoding pectin methyl esterase inhibitor. Two other genes, designated WFI_6BL1 and WFI_6BL2 (wheat-Fusarium interaction genes 6BL1 and 6BL2), were functionally associated with Fhb_6BL but physically mapped on chromosomes 7D and 5A, respectively. WFI_6BL1 was annotated as a 13- lipoxygenase gene and WFI_6BL2 might encode a PR-4-like protein. Our data suggested that i) Fhb1 seems to contribute to FHB resistance by reducing susceptibility in the first 60 h, ii) Fhb_6BL makes its contribution via the jasmonate-mediated pathways, and iii) wheat seems to activate its defense mechanism in the biotrophic phase of FHB pathogenesis.

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Mapping of Quantitative Trait Loci for Fusarium Head Blight Resistance in Barley

Phytopathology ◽

10.1094/phyto.2000.90.10.1079 ◽

2000 ◽

Vol 90 (10) ◽

pp. 1079-1088 ◽

Cited By ~ 78

Author(s):

Zhengqiang Ma ◽

Brian J. Steffenson ◽

Louis K. Prom ◽

Nora L. V. Lapitan

Keyword(s):

Quantitative Trait Loci ◽

Fusarium Head Blight ◽

Plant Height ◽

Quantitative Trait ◽

Morphological Traits ◽

Heading Date ◽

Rflp Markers ◽

Head Blight ◽

Trait Loci ◽

Fhb Resistance

Fusarium head blight (FHB) is a devastating disease that causes significant reductions in yield and quality in wheat and barley. Barley grains infected with deoxynivalenol (DON), a vomitoxin produced by Fusarium graminearum, are rejected for malting and brewing. Among six-rowed barley cultivars tested thus far, only cv. Chevron exhibited resistance. This study was conducted to map genes and to identify DNA markers for marker-assisted breeding for FHB resistance in cv. Chevron with restriction fragment length polymorphism (RFLP) markers. A doubled haploid (DH) population was created from a cross between cv. Chevron and susceptible cv. Stander. Seven field experiments were conducted in four different locations in 2 years. A RFLP map containing 211 loci and covering over 1,000 centimorgans (cM) of the genome was used to map quantitative trait loci (QTL) associated with relatively low FHB severity and DON concentration. Morphological traits differing between the parents were also measured: heading date, plant height, spike angle, number of nodes per cm of rachis in the spike, and kernel plumpness. Many of the QTL for FHB and DON coincided with QTLs for these morphological traits. The “fix-QTL” algorithm in Mapmaker QTL was used to remove the part of the variance for FHB resistance that may be explained by heading date or plant height. Results from this study suggest that QTLs with major effects for FHB resistance probably do not exist in cv. Chevron. Three QTL intervals, Xcmwg706-Xbcd441 on chromosome 1H, Xbcd307b-Xcdo684b on chromosome 2H, and Xcdo959b-Xabg472 on chromosome 4H, that are not associated with late heading or height may be useful for marker-assisted selection.

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