High efficiency and reliability of inter-simple sequence repeats (ISSR) markers for evaluation of genetic diversity in Brazilian cultivated Jatropha curcas L. accessions

2010 ◽  
Vol 38 (7) ◽  
pp. 4245-4256 ◽  
Author(s):  
Clícia Grativol ◽  
Catarina da Fonseca Lira-Medeiros ◽  
Adriana Silva Hemerly ◽  
Paulo Cavalcanti Gomes Ferreira
Keyword(s):  
Genetic Diversity ◽  
Simple Sequence Repeats ◽  
Jatropha Curcas ◽  
High Efficiency ◽  
Issr Markers ◽  
Jatropha Curcas L ◽  
Inter Simple Sequence Repeats ◽  
Efficiency And Reliability ◽  
Simple Sequence

scholarly journals Genetic diversity and phenetic relationships of five Trifolium L. species (Fabaceae) by inter simple sequence repeats markers

2016 ◽  
Vol 23 (2) ◽  
pp. 167-173
Author(s):  
Yourang Hwang ◽  
Man Kyu Huh
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Gene Flow ◽  
Simple Sequence Repeats ◽  
Issr Markers ◽  
Phenetic Relationships ◽  
Inter Simple Sequence Repeats ◽  
Total Genetic Diversity ◽  
Plant Taxon ◽  
Simple Sequence

Five species of Trifolium L. (T. repens L., T. pretense L., T. hybridum L., T. campestre Schreb., and T. dubium Sibth.) were analyzed used to evaluate the genetic diversity and their phenetic relationships using inter-simple sequence repeats (ISSR) markers. Overall, T. pratense exhibited higher variation than other species. 114 amplicons were produced by ISSR markers, of which 77 (67.5%) bands were polymorphic. T. dubium showed the low genetic variation. Total genetic diversity values (HT) varied between 0.333 and 0.487, for an average over all polymorphic loci of 0.282. On a perlocus basis, the proportion of total genetic variation due to differences among species (GST) was 0.380. This indicated that about 38.0% of the total variation was among species. The estimate of gene flow, based on GST, was very low among species of genus Trifolium (Nm = 0.816). An assessment of the proportion of diversity present within species, HPOP/HSP, indicated that about 95.8% the total genetic diversity was within species. T. pratense and T. hybridum were grouped together and this clade was sister with T. repens. Two remainder species with yellow flowers were grouped together. Information on genetic diversity for Trifolium is valued for the management of germplasm and for evolving conservation strategies.Bangladesh J. Plant Taxon. 23(2): 167-173, 2016 (December)

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