Ternary Complexes with Core-Shell Bilayer for Double Level Targeted Gene Delivery: In Vitro and In Vivo Evaluation

It requires multistep synthesis and conjugation processes to incorporate multifunctionalities into a polyplex gene vehicle to overcome numerous hurdles during gene delivery. Here, we describe a supramolecular platform to precisely control, screen, and optimize molecular architectures of siRNA targeted delivery vehicles, which is based on rationally designed host-guest complexation between a β-cyclodextrin–based cationic host polymer and a library of guest polymers with various PEG shape and size, and various density of ligands. The host polymer is responsible to load/unload siRNA, while the guest polymer is responsible to shield the vehicles from nonspecific cellular uptake, to prolong their circulation time, and to target tumor cells. A series of precisely controlled molecular architectures through a simple assembly process allow for a rapid optimization of siRNA delivery vehicles in vitro and in vivo for therapeutic siRNA-Bcl2 delivery and tumor therapy, indicating the platform is a powerful screening tool for targeted gene delivery vehicles.

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Receptor-Mediated Gene Delivery Using Chitosan Derivatives In Vitro and In Vivo

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.342-343.449 ◽

2007 ◽

Vol 342-343 ◽

pp. 449-452 ◽

Cited By ~ 1

Author(s):

Tae Hee Kim ◽

Hua Jin ◽

Hyun Woo Kim ◽

Myung Haing Cho ◽

Jae Woon Nah ◽

...

Keyword(s):

Gene Delivery ◽

Delivery System ◽

Transfection Efficiency ◽

Viral Gene ◽

Gene Delivery System ◽

Targeted Gene Delivery ◽

Cell Specificity ◽

Selective Delivery

The key strategy for the advancement of gene therapy is the development of an efficient targeted gene delivery system into cells. The targeted gene delivery system is especially important in non-viral gene transfer which shows the relatively low transfection efficiency. It also opens the possibility of selective delivery of therapeutic plasmids to specific tissues. Chitosan has been considered to be a good candidate for gene delivery system, since it is already known as a biocompatible, biodegradable, and low toxic material with high cationic potential. However, low specificity and low transfection efficiency of chitosan need to be overcome prior to clinical trial. In this study, we focused on the chemical modification of chitosan for enhancement of cell specificity and transfection efficiency. Also, the potential of clinical application was investigated.

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Nevirapine Loaded Core Shell Gold Nanoparticles by Double Emulsion Solvent Evaporation: In vitro and In vivo Evaluation

Current Drug Delivery ◽

10.2174/1567201813666160114093005 ◽

2016 ◽

Vol 13 (7) ◽

pp. 1071-1083 ◽

Cited By ~ 3

Author(s):

Bhagyashree R. Dalvi ◽

Ejaz A. Siddiqui ◽

Asad S. Syed ◽

Shilpa M. Velhal ◽

Absar Ahmad ◽

...

Keyword(s):

Gold Nanoparticles ◽

Double Emulsion ◽

Solvent Evaporation ◽

Core Shell ◽

In Vivo Evaluation

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Receptor-Mediated Gene Delivery Using Chitosan Derivatives In Vitro and In Vivo

Materials Science Forum ◽

10.4028/www.scientific.net/msf.539-543.641 ◽

2007 ◽

Vol 539-543 ◽

pp. 641-646 ◽

Cited By ~ 1

Author(s):

Tae Hee Kim ◽

Jin Hua ◽

Hyun Woo Kim ◽

Myung Haing Cho ◽

Jae Woon Nah ◽

...

Keyword(s):

Gene Delivery ◽

Delivery System ◽

Transfection Efficiency ◽

Viral Gene ◽

Gene Delivery System ◽

Targeted Gene Delivery ◽

Cell Specificity ◽

Selective Delivery

The development of an efficient targeted gene delivery system into cells is an important strategy for the advancement of gene therapy. The targeted gene delivery system is especially important in non-viral gene transfer which shows the relative low transfection efficiency. And it also opens the possibility of selective delivery of therapeutic plasmids to specific tissues. Chitosan has been considered to be a good candidate for gene delivery system, since it is already known as a biocompatible, biodegradable, and low toxic material with high cationic potential. However, low specificity and low transfection efficiency of chitosan need to be overcome prior to clinical trial. In this study, we focused on the chemical modification of chitosan for enhancement of cell specificity and transfection efficiency.

Download Full-text