scholarly journals How do different nitrogen application levels and irrigation practices impact biological nitrogen fixation and its distribution in paddy system?

2021 ◽  
Author(s):  
Yanhui Zhang ◽  
Tianlong Hu ◽  
Hui Wang ◽  
Haiyang Jin ◽  
Qi Liu ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Biological Nitrogen Fixation ◽  
Nitrogen Application ◽  
Biological Nitrogen ◽  
Irrigation Practices

scholarly journals Nitrogen fixating ability of mungbean genotypes under different levels of nitrogen application

2016 ◽  
Vol 41 (1) ◽  
pp. 163-171 ◽  
Author(s):  
MA Razzaque ◽  
MM Haque ◽  
MA Karim ◽  
ARM Solaiman
Keyword(s):  
Nitrogen Fixation ◽  
Seed Yield ◽  
Biological Nitrogen Fixation ◽  
Yield Potential ◽  
Nitrogen Application ◽  
Culture Experiment ◽  
N Application ◽  
Biological Nitrogen ◽  
Different Levels ◽  
Pot Culture

A pot culture experiment was conducted at the Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur during kharif II, 2012 to evaluate the nodulation, biological nitrogen fixation and yield potential of genotypes of mungbean under varying levels of N application. There were 10 mungbean genotypes viz. IPSA 12, GK 27, IPSA 3, IPSA 5, ACC12890055, GK 63, ACC12890053, BU mug 4, BARI Mung 6 and Binamoog 5, each genotype treated with six levels of N (0, 20, 40, 60, 80 and 100 kg N ha-1) . Among the genotypes, the IPSA 12 at 40 kg N ha-1 produced the maximum number of nodules (14.54 plant-1) as well as the highest nitrogen fixation (2.684 mmol C2H4). This resulted in the highest seed yield (14.22 g plant-1). The genotype ACC12890053 recorded the lowest nodulation (6 plant-1), nitrogen fixation (1.134) and seed yield (7.33 g plant-1).Bangladesh J. Agril. Res. 41(1): 163-171, March 2016

scholarly journals How Do Different Nitrogen Application Levels and Irrigation Practices Impact Biological Nitrogen Fixation and its Distribution in Paddy System?

2021 ◽  
Author(s):  
Yanhui Zhang ◽  
Tianlong Hu ◽  
Hui Wang ◽  
Haiyang Jin ◽  
Qi Liu ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Biological Nitrogen Fixation ◽  
Soil Depth ◽  
Soil Layer ◽  
Nitrogen Application ◽  
High Nitrogen ◽  
Fixed Nitrogen ◽  
Intermittent Irrigation ◽  
Biological Nitrogen ◽  
Labelling System

Abstract Background and aimsBiological nitrogen fixation (BNF) in paddy systems is impacted by nitrogen application levels and irrigation strategies, but the extents to which these factores influence BNF and its distribution in soil and rice were still largely unclear. This study investigates this influence.MethodsAn airtight transparent growth chamber 15N-labelling system were used to investigate how different nitrogen application levels (0, 125, 187.5 and 250 kg N ha-1) and irrigation strategies (flooding irrigation or intermittent irrigation) impact the amount of BNF and its distribution in soil and rice. ResultsNitrogen application at 125 ~ 250 kg N ha-1 reduced the amount of BNF by 81% - 86%. The inhibition effect of nitrogen application on BNF at a soil depth of 1-15 cm was greater than that at 0-1 cm. Relative to the continuous flooding irrigation, intermittent irrigation enhanced rice growth and promoted the transfer of fixed nitrogen from 0-1 cm soil layer to rice, but it did not change the total amount of BNF. ConclusionsThis study indicated that BNF supplied little nitrogen for rice production at the high nitrogen application levels, but the intermittent irrigation could promote utilization of biologically fixed nitrogen.

scholarly journals Functional analysis of multiple nifB genes of Paenibacillus strains in synthesis of Mo-, Fe- and V-nitrogenases

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Qin Li ◽  
Haowei Zhang ◽  
Liqun Zhang ◽  
Sanfeng Chen
Keyword(s):  
Functional Analysis ◽  
Nitrogen Fixation ◽  
Gene Cluster ◽  
Biological Nitrogen Fixation ◽  
Deletion Analysis ◽  
Phylogeny Analysis ◽  
Biological Nitrogen

Abstract Background Biological nitrogen fixation is catalyzed by Mo-, V- and Fe-nitrogenases that are encoded by nif, vnf and anf genes, respectively. NifB is the key protein in synthesis of the cofactors of all nitrogenases. Most diazotrophic Paenibacillus strains have only one nifB gene located in a compact nif gene cluster (nifBHDKENX(orf1)hesAnifV). But some Paenibacillus strains have multiple nifB genes and their functions are not known. Results A total of 138 nifB genes are found in the 116 diazotrophic Paenibacillus strains. Phylogeny analysis shows that these nifB genes fall into 4 classes: nifBI class including the genes (named as nifB1 genes) that are the first gene within the compact nif gene cluster, nifBII class including the genes (named as nifB2 genes) that are adjacent to anf or vnf genes, nifBIII class whose members are designated as nifB3 genes and nifBIV class whose members are named as nifB4 genes are scattered on genomes. Functional analysis by complementation of the ∆nifB mutant of P. polymyxa which has only one nifB gene has shown that both nifB1 and nifB2 are active in synthesis of Mo-nitrogenase, while nifB3 and nifB4 genes are not. Deletion analysis also has revealed that nifB1 of Paenibacillus sabinae T27 is involved in synthesis of Mo-nitrogenase, while nifB3 and nifB4 genes are not. Complementation of the P. polymyxa ∆nifBHDK mutant with the four reconstituted operons: nifB1anfHDGK, nifB2anfHDGK, nifB1vnfHDGK and nifB2vnfHDGK, has shown both that nifB1 and nifB2 were able to support synthesis of Fe- or V-nitrogenases. Transcriptional results obtained in the original Paenibacillus strains are consistent with the complementation results. Conclusions The multiple nifB genes of the diazotrophic Paenibacillus strains are divided into 4 classes. The nifB1 located in a compact nif gene cluster (nifBHDKENX(orf1)hesAnifV) and the nifB2 genes being adjacent to nif or anf or vnf genes are active in synthesis of Mo-, Fe and V-nitrogenases, but nifB3 and nifB4 are not. The reconstituted anf system comprising 8 genes (nifBanfHDGK and nifXhesAnifV) and vnf system comprising 10 genes (nifBvnfHDGKEN and nifXhesAnifV) support synthesis of Fe-nitrogenase and V-nitrogenase in Paenibacillus background, respectively.

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