Ovary co-culture improves embryo and green plant production in anther culture of Australian spring wheat (Triticum aestivum L.)

2008 ◽  
Vol 95 (2) ◽  
pp. 185-195 ◽  
Author(s):  
Sue Broughton
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Spring Wheat ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
Plant Production

The application of n-butanol improves embryo and green plant production in anther culture of Australian wheat (Triticum aestivum L.) genotypes

2011 ◽  
Vol 62 (10) ◽  
pp. 813 ◽  
Author(s):  
Sue Broughton
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Doubled Haploids ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
Plant Production ◽  
Wheat Cultivars ◽  
Green Plants ◽  
Australian Wheat ◽  
New Treatments

The objective of this study was to improve the production from anther culture of embryos and green plants in Australian spring wheat genotypes by testing new treatments such as n-butanol, as well as other protocol modifications. To date, the use of n-butanol to enhance embryogenesis has only been tested in two European wheat cultivars; this is the first study which demonstrates its application across a range of breeding crosses. A 5-h treatment using 0.1 or 0.2% (v/v) n-butanol following anther pretreatment on a solid mannitol medium significantly improved the production of embryos, green plants and doubled haploids in a range of Australian wheat crosses and varieties. Green plant production increased between 3- and 6-fold in the crosses Yitpi/2*Bumper, Tammarin Rock/2*Bumper and Tammarin Rock/2*Magenta. The addition of calcium (Ca) and macronutrients to the mannitol pretreatment medium also significantly improved the number of embryos and green plants in varieties and crosses, but only when used in combination with n-butanol treatment. A factorial experiment with four varieties and two treatments (n-butanol and Ca/macronutrients) revealed significant interactions between treatments and genotype. In three of the four varieties, the application of n-butanol resulted in significant increases in embryos and green plants with either pretreatment medium although the best results were obtained with Ca and macronutrients in the pretreatment medium, with 200, 193 and 52 green plants per 100 anthers obtained for Bumper, Gladius and Magenta, respectively. In the variety Fortune however, n-butanol treatment did not improve embryo or green plant production unless it was combined with Ca and macronutrients in the pretreatment medium and then there were dramatic improvements; from 0 to 27 green plants per 100 anthers.

Reciprocal substitutions analysis of embryo induction and plant regeneration from anther culture in wheat (Triticum aestivum L.)

Genome ◽  
1995 ◽  
Vol 38 (1) ◽  
pp. 158-165 ◽  
Author(s):  
M. Ghaemi ◽  
A. Sarrafi ◽  
R. Morris
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
Plant Production ◽  
Reciprocal Effects ◽  
Substitution Lines ◽  
Reciprocal Effect ◽  
Total Plant

Reciprocal substitutions for all chromosomes between the hard red winter wheat cultivars Wichita and Cheyenne were used to investigate the effects of individual chromosomes, as well as their interactions with the genetic background, on androgenesis. Duplicate lines for each chromosome were included to check background homogeneity. Six experiments, two for each genome, were performed. In each experiment, 14 substitution lines, their 14 duplicate lines, and the two parental genotypes ('Cheyenne' and 'Wichita') were studied. The experimental design was a randomized block with three replications. 'Wichita' and 'Cheyenne' differed significantly in embryo yield and green plant regeneration (except green plant regeneration for the B-genome tests) and were equal for albino and total plant regeneration. Embryogenesis was influenced by some chromosomes of the A, B, and D genomes; green plant production was influenced by all chromosomes of the A and D genomes except 5D; albino and total plant regeneration were affected by some chromosomes of the B and D genomes. Reciprocal effects were obtained with chromosomes 1A, 7A, 1B, 5B, 1D, and 2D for embryogenesis, chromosomes 2D and 7D for green plant regeneration, and chromosome 2D for total plant regeneration. Reciprocal substitution lines revealed reciprocal effects of homologous chromosomes, as well as interactions between substituted chromosomes and their specific genetic background.Key words: anther culture, reciprocal effect, substitution lines, Triticum aestivum, embryogenesis.

scholarly journals Efficiency of anther culture technique in the production of wheat double haploids

2008 ◽  
pp. 35-40 ◽  
Author(s):  
Ankica Kondic-Spika ◽  
Borislav Kobiljski ◽  
Nikola Hristov
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
Culture Technique ◽  
Average Yield ◽  
Green Plants ◽  
Double Haploids

The objective of the study was to investigate efficiency of anther culture in the production of spontaneous double haploids from randomly selected heterozygous genotypes of wheat (Triticum aestivum L.). Anthers of 20 F1 wheat combinations were grown in vitro on a modified Potato-2 medium. All of the examined genotypes have shown the ability to produce pollen calluses as well as to regenerate green plants. On average for the whole experiment material, 47.2 calluses were produced per 100 cultured anthers. The green plant regeneration ranged from 0.8 to 13.4 green plants per spike, with an overall mean of 5.8. From the total of 582 regenerated green plants, 47.9% (279) were spontaneous double haploids. The final average yield from the study was 2.8 double haploids per spike.

scholarly journals Androgenous ability of heterozygous wheat genotypes and cytological characteristics of green regenerants

Genetika ◽  
2006 ◽  
Vol 38 (2) ◽  
pp. 153-158
Author(s):  
Branka Ljevnaic ◽  
Ankica Kondic-Sipka ◽  
Borislav Kobiljski ◽  
Srbislav Dencic
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
Regeneration Ability ◽  
Cytological Analysis ◽  
Green Plants ◽  
Albino Plants ◽  
Cytological Characteristics

Androgenous and regeneration abilities of 8 heterozygous wheat (Triticum aestivum L.) genotypes were analyzed in anther culture. Cytological characteristics of green regenerants were also analyzed. Significant differences were found among the genotypes in their androgenous and regeneration abilities. The average androgenous capacity of all genotypes was 4.4% ranging from 0.7% (Prima/Hueguen) to 11.0% (NS173-98/NS164-98). The average callus yield was 8.7%. The genotype Banks/F53-70 produced the highest number of green plants (33 green plants), while the genotype Banks/Pobeda showed no green plant regeneration ability. The frequency of albino plants ranged between 0% (Banks/Rodna) and 5.3% (Anastasija/Radika). A total of 61 green and 61 albino plants were regenerated. The results of cytological analysis showed that, from the total number of regenerated green plants, 40.9% were haploids and 59.1% were spontaneous double haploids.

scholarly journals The Role of Drought Stress on Anther Culture of Wheat (Triticum aestivum L.)

2010 ◽  
Vol 20 (1) ◽  
pp. 55-61 ◽  
Author(s):  
S. M. Shahinul Islam
Keyword(s):  
Triticum Aestivum ◽  
Drought Stress ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Plant Production ◽  
Albino Plant ◽  
Albino Plants ◽  
Embryo Yield ◽  
Embryo Regeneration

Drought stress was found to pose a significant effect on anther culture of wheat, namely Barkat, Kanchan and Pavon-76. Regeneration potentials of these varieties were determined by estimating the percentage of anther response, embryo induction, embryo regeneration and production of green and albino plants. It was observed that out of five treatments such as T1 (1 hr), T2 (3 hr), T3 (5 hr), T4 (7 hr) and T5 (9 hr) only T2 showed highest percentage of embryo yield and green plantlets. The T1 and T3 also gave significantly better results compared to the control. All the genotypes produced embryos and green plantlets and of them Barkat showed best performance followed by Kanchan and Pavon 76. Genotypes, under this study, produced green plants in addition to albinos but T4 and T5 showed three - fourfold higher albino plant production in comparison to treatments.  Key words: Anther culture, Drought stress, Haploids, Triticum aestivum D.O.I. 10.3329/ptcb.v20i1.5965 Plant Tissue Cult. & Biotech. 20(1): 55-61, 2010 (June)

THE INFLUENCE OF VARIOUS CULTURAL FACTORS ON ANTHER CULTURE OF FOUR CULTIVARS OF SPRING WHEAT (Triticum aestivum L.)

1990 ◽  
Vol 70 (1) ◽  
pp. 183-191 ◽  
Author(s):  
LINDA J. McGREGOR ◽  
ALAN McHUGHEN
Keyword(s):  
Anther Culture ◽  
Spring Wheat ◽  
Callus Induction ◽  
Incubation Temperature ◽  
Callus Formation ◽  
Synthetic Medium ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
Cold Pretreatment ◽  
Agar Concentration

Four spring wheat cultivars (Katepwa, Columbus, Chris and Israel 676) were compared for androgenic ability. Temperature, medium, agar concentration and cold pretreatment were examined to find the optimal combinations for anther culture of Katepwa and Columbus in particular. At an incubation temperature of 30 °C, anthers of Chris were significantly more responsive than the other genotypes. In three temperature treatments (26, 28 and 30 °C) the potato extract medium (P1) was superior to the synthetic medium (N6) (Chu 1978) for callus induction and green plant regeneration. Over all genotypes, liquid media provided greater anther response than either 0.8% or 1.8% agar. At 28 °C, cultured anthers showed no significant increase in callus formation after being subjected to a cold pretreatment. A cold pretreatment prior to culture at 30 °C elicited a significant decrease in callus formation as compared with no cold pretreatment. However, maximum callus induction and regeneration frequencies for Katepwa and Columbus were obtained at 28 °C in the P1 liquid medium with cold pretreatment. An incubation temperature of 26 °C was inferior to 28° and 30 °C for callus induction and regeneration.Key words: Anther culture, wheat, genotype, incubation temperature, media, agar concentration

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