scholarly journals Spectroscopic and biochemical characteristics of flax transgenic callus cultures producing PHB

2020 ◽  
Vol 141 (3) ◽  
pp. 489-497 ◽  
Author(s):  
Magdalena Wróbel-Kwiatkowska ◽  
Kamil Kostyn ◽  
Lucyna Dymińska ◽  
Jerzy Hanuza ◽  
Adam Kurzawa ◽  
...  
Keyword(s):  
Callus Cultures ◽  
Biochemical Characteristics ◽  
Transgenic Callus

The production of class III plant peroxidases in transgenic callus cultures transformed with the rolB gene of Agrobacterium rhizogenes

2013 ◽  
Vol 168 (1) ◽  
pp. 64-70 ◽  
Author(s):  
Y.N. Shkryl ◽  
G.N. Veremeichik ◽  
V.P. Bulgakov ◽  
T.V. Avramenko ◽  
E.A. Günter ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Callus Cultures ◽  
Class Iii ◽  
Rolb Gene ◽  
Plant Peroxidases ◽  
Transgenic Callus

Microtubule Dynamics and Organelle Transport in Reticulomyxa

1990 ◽  
Vol 48 (3) ◽  
pp. 194-195
Author(s):  
Yih-Tai Chen ◽  
Ursula Euteneuer ◽  
Ken B. Johnson ◽  
Michael P. Koonce ◽  
Manfred Schliwa
Keyword(s):  
Plasma Membrane ◽  
Light Microscopy ◽  
Cytoplasmic Dynein ◽  
Living Cells ◽  
Microtubule Dynamics ◽  
Model Systems ◽  
Organelle Transport ◽  
Biochemical Characteristics ◽  
Dependent Transport

The application of video techniques to light microscopy and the development of motility assays in reactivated or reconstituted model systems rapidly advanced our understanding of the mechanism of organelle transport and microtubule dynamics in living cells. Two microtubule-based motors have been identified that are good candidates for motors that drive organelle transport: kinesin, a plus end-directed motor, and cytoplasmic dynein, which is minus end-directed. However, the evidence that they do in fact function as organelle motors is still indirect.We are studying microtubule-dependent transport and dynamics in the giant amoeba, Reticulomyxa. This cell extends filamentous strands backed by an extensive array of microtubules along which organelles move bidirectionally at up to 20 μm/sec (Fig. 1). Following removal of the plasma membrane with a mild detergent, organelle transport can be reactivated by the addition of ATP (1). The physiological, pharmacological and biochemical characteristics show the motor to be a cytoplasmic form of dynein (2).

SEM and fluorescence imaging of callose deposition in root hair tips and suspension cultures of Streptanthus tortuosus

1990 ◽  
Vol 48 (3) ◽  
pp. 698-699
Author(s):  
K.S. Walters ◽  
R.D. Sjolund ◽  
K.C. Moore
Keyword(s):  
Cell Wall ◽  
Root Hair ◽  
Cultured Cells ◽  
Root Hairs ◽  
Callus Cultures ◽  
Callose Deposition ◽  
Culture Cells ◽  
Wall Structures ◽  
Ultraviolet Illumination ◽  
Callose Formation

Callose, B-1,3-glucan, a component of cell walls, is associated with phloem sieve plates, plasmodesmata, and other cell wall structures that are formed in response to wounding or infection. Callose reacts with aniline blue to form a fluorescent complex that can be recognized in the light microscope with ultraviolet illumination. We have identified callose in cell wall protuberances that are formed spontaneously in suspension-cultured cells of S. tortuosus and in the tips of root hairs formed in sterile callus cultures of S. tortuosus. Callose deposits in root hairs are restricted to root hair tips which appear to be damaged or deformed, while normal root hair tips lack callose deposits. The callose deposits found in suspension culture cells are restricted to regions where unusual outgrowths or protuberances are formed on the cell surfaces, specifically regions that are the sites of new cell wall formation.Callose formation has been shown to be regulated by intracellular calcium levels.

Abstract #803: Cushing Disease Due to Pituitary Macroadenoma: Biochemical Characteristics in a Pediatric Cohort

2015 ◽  
Vol 21 ◽  
pp. 148-149
Author(s):  
Ricardo Correa ◽  
Maria Batsis ◽  
Prashant Chittiboina ◽  
Pooja Raghavan ◽  
Elena Belyavskaya ◽  
...  
Keyword(s):  
Pituitary Macroadenoma ◽  
Cushing Disease ◽  
Biochemical Characteristics

Abstract #856 A Case Series Review of Clinical and Biochemical Characteristics of Pituitary Abcess in Mexican Patients

2018 ◽  
Vol 24 ◽  
pp. 202-203
Author(s):  
Mireya Perez-Guzman ◽  
Alfredo Nava de la Vega ◽  
Arturo Pena Velarde ◽  
Tania Raisha Torres Victoria ◽  
Froylan Martinez-Sanchez ◽  
...  
Keyword(s):  
Case Series ◽  
Biochemical Characteristics ◽  
Mexican Patients
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