Molecular detection of piroplasms in ixodid ticks infesting cattle and sheep in western Oromia, Ethiopia

2013 ◽  
Vol 46 (1) ◽  
pp. 27-31 ◽  
Author(s):  
Bersissa Kumsa ◽  
Manuela Signorini ◽  
Sori Teshale ◽  
Cinzia Tessarin ◽  
Reta Duguma ◽  
...  
2016 ◽  
Vol 7 (5) ◽  
pp. 734-741 ◽  
Author(s):  
Kifaya Azmi ◽  
Suheir Ereqat ◽  
Abedelmajeed Nasereddin ◽  
Amer Al-Jawabreh ◽  
Gad Baneth ◽  
...  

2012 ◽  
Vol 74 (12) ◽  
pp. 1551-1560 ◽  
Author(s):  
Adrian Patalinghug YBA^|^Ntilde;EZ ◽  
Kotaro MATSUMOTO ◽  
Toshio KISHIMOTO ◽  
Naoaki YOKOYAMA ◽  
Hisashi INOKUMA

2014 ◽  
Vol 40 (3) ◽  
pp. 772-773
Author(s):  
Mehdi Aghamohammad Hassan ◽  
Afshin Raoofi ◽  
Arman Hosseini ◽  
Mohammad Reza Mehrara ◽  
Fatemeh Amininajafi

2013 ◽  
Vol 13 (1) ◽  
pp. 6-11 ◽  
Author(s):  
Adrian P. Ybañez ◽  
Michihito Tagawa ◽  
Kotaro Matsumoto ◽  
Toshio Kishimoto ◽  
Naoaki Yokoyama ◽  
...  

2018 ◽  
Vol 11 (4) ◽  
pp. 431-436 ◽  
Author(s):  
Hamza Jawad Al-Ali ◽  
Mohsen Abd Al-Rodhan ◽  
Samer Abdulsahib Al-Hilali ◽  
Alaa Hani Al-Charrakh ◽  
Ali Muhsin Al-Mohana ◽  
...  

Author(s):  
Sedighe MIR ◽  
Mansour DABIRZADEH ◽  
Mohammad Bagher ROKNI ◽  
Mojgan ARYAEIPOUR ◽  
Mahdi KHOSHSIMA SHAHRAKI ◽  
...  

Background: The detection of Fasciola species in various geographical regions is essential for health policymaking. Here, we aimed to identify livestock (cattle and sheep) related Fasciola genotypes by restriction fragment length polymorphism PCR. Methods: Seventy adult Fasciola flukes were collected from 70 infected livers of 35 cattle and 35 sheep slaughtered in Zabol abattoir, outh-east Iran (Jan-Jul 2017). Fasciola species were determined based on molecular features. For molecular detection, Fasciola ITS1 region was amplified and sequenced. A 700 bp fragment was amplified. These were digested with RasΙ enzyme. F. hepatica specific fragments were 47, 59, 68, 104, and 370, while those related to F. gigantica had 45, 55, 170, 370. Results: The two main species of F. hepatica and F. gigantica are responsible for fasciolosis in sheep and cattle in our region. From 35 Fasciola isolated from cattle, 3 and 32 were F. hepatica and F. giagantica respectively. From 35 Fasciola isolated from sheep, 4 were F. hepatica and 31 were F. gigantica. Conclusion: All Seventy Fasciola samples from two different hosts (cattle and sheep) were identified as either F. hepatica or F. gigantica by PCR-RFLP. Genotypic variability of Fasciola species was high in our region. It is recommended to assess molecular variation of Fasciola isolates in other host livestock.


2010 ◽  
Vol 43 (2) ◽  
pp. 459-461 ◽  
Author(s):  
Mohammad Mirzaei Dehaghi ◽  
Saeid Fathi ◽  
Ehsan Norouzi Asl ◽  
Hojat Asgary nezhad

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