Improved molecular detection of Ehrlichia and Anaplasma species applied to Amblyomma ticks collected from cattle and sheep in Ethiopia

Background: The detection of Fasciola species in various geographical regions is essential for health policymaking. Here, we aimed to identify livestock (cattle and sheep) related Fasciola genotypes by restriction fragment length polymorphism PCR. Methods: Seventy adult Fasciola flukes were collected from 70 infected livers of 35 cattle and 35 sheep slaughtered in Zabol abattoir, outh-east Iran (Jan-Jul 2017). Fasciola species were determined based on molecular features. For molecular detection, Fasciola ITS1 region was amplified and sequenced. A 700 bp fragment was amplified. These were digested with RasΙ enzyme. F. hepatica specific fragments were 47, 59, 68, 104, and 370, while those related to F. gigantica had 45, 55, 170, 370. Results: The two main species of F. hepatica and F. gigantica are responsible for fasciolosis in sheep and cattle in our region. From 35 Fasciola isolated from cattle, 3 and 32 were F. hepatica and F. giagantica respectively. From 35 Fasciola isolated from sheep, 4 were F. hepatica and 31 were F. gigantica. Conclusion: All Seventy Fasciola samples from two different hosts (cattle and sheep) were identified as either F. hepatica or F. gigantica by PCR-RFLP. Genotypic variability of Fasciola species was high in our region. It is recommended to assess molecular variation of Fasciola isolates in other host livestock.

Download Full-text

Molecular detection and phylogenetic analysis of ORF103 and P32 genes of Capripoxviruses isolated from naturally infected cattle and sheep from Kaliobyia province in Egypt.

Benha Veterinary Medical Journal ◽

10.21608/bvmj.2021.79378.1429 ◽

2021 ◽

Vol 41 (1) ◽

pp. 54-50

Author(s):

Shimaa Abd-Elhafeiz ◽

Ayman El-Habbaa ◽

Fouad El-mayet ◽

Lamya Ateya ◽

Ehab EL-Nahas

Keyword(s):

Phylogenetic Analysis ◽

Molecular Detection ◽

Infected Cattle ◽

Cattle And Sheep

Download Full-text

Molecular detection of rickettsial agents of hard ticks (Acari: Ixodidae) collected from wild birds of Panama

Systematic and Applied Acarology ◽

10.11158/saa.25.4.3 ◽

2020 ◽

Vol 25 (4) ◽

pp. 622-632

Author(s):

Sergio Bermúdez ◽

Lillian Domínguez ◽

Isis Ochoa ◽

Jonathan Gonçalves Oliveira ◽

Elba Regina Sampaio De Lemos ◽

...

Keyword(s):

Molecular Detection ◽

Wild Birds ◽

Hard Ticks ◽

Amblyomma Ovale ◽

Amblyomma Ticks

Ticks (Acari: Ixodidae) collected from wild birds in Panama were tested for the presence of tick-borne pathogens as Rickettsia, Anaplasma, Ehrlichia, Bartonella, Borrelia, Hepatozoon sp., and Babesia. Overall 124 ticks were found in 57 birds belonging to 28 species: Amblyomma longirostre (32 larvae, 1 nymph), Amblyomma nodosum (30 nymphs), Amblyomma geayi (15 larvae, 1 nymph), Amblyomma varium (5 larvae, 2 nymphs), Amblyomma naponense (2 larvae), Amblyomma ovale (2 larvae), and Amblyomma calcaratum (1 larva). DNA of Rickettsia amblyommatis was detected in 65% of A. longirostre, 69% of A. geayi and 14% of A. varium. Moreover, results from two larvae of A. longirostre showed DNA of unidentified Rickettsia sp. No DNA of Borrelia, Bartonella, Anaplasmataceae neither Babesia nor Hepatozoon was detected. These results expand knowledge about the host for immature Amblyomma ticks in Panama and show the first data of Rickettsia in ticks collected from birds in this country.

Download Full-text

Molecular detection of piroplasms in ixodid ticks infesting cattle and sheep in western Oromia, Ethiopia

Tropical Animal Health and Production ◽

10.1007/s11250-013-0442-z ◽

2013 ◽

Vol 46 (1) ◽

pp. 27-31 ◽

Cited By ~ 12

Author(s):

Bersissa Kumsa ◽

Manuela Signorini ◽

Sori Teshale ◽

Cinzia Tessarin ◽

Reta Duguma ◽

...

Keyword(s):

Molecular Detection ◽

Ixodid Ticks ◽

Cattle And Sheep

Download Full-text

Molecular detection of bovine leukemia virus in peripheral blood of Iranian cattle, camel and sheep

Polish Journal of Veterinary Sciences ◽

10.1515/pjvs-2015-0091 ◽

2015 ◽

Vol 18 (4) ◽

pp. 703-707 ◽

Cited By ~ 9

Author(s):

S. Nekoei ◽

T. Taktaz Hafshejani ◽

A. Doosti ◽

F. Khamesipour

Keyword(s):

B Lymphocytes ◽

Peripheral Blood ◽

Bovine Leukemia Virus ◽

Blood Circulation ◽

Molecular Detection ◽

Leukemia Virus ◽

Blood Samples ◽

Baseline Information ◽

Peripheral Blood Circulation ◽

Cattle And Sheep

Abstract Bovine leukemia virus (BLV) is a deltaretrovirus which infects and induces proliferation of B-lymphocytes in the peripheral blood circulation and in lymphoid organs primarily of cattle, leading to leukemia/lymphoma. This study was carried out to investigate the presence of BLV in cattle, sheep and camels from the Chaharmahal va Bakhtiary and Isfahan provinces in Iran. A total of 874 blood samples collected from cattle, sheep and camels were used in this study to detect BLV using a nested-PCR. The results from this study indicated that 17.2% (n=874) of all blood samples collected were positive for BLV. The percentages of blood samples positive for BLV from cattle, sheep and camels were 22.1 (n=657), 5.3 (n=95) and 0 (n=122) respectively. The results from this study showed that BLV infected cattle and sheep. Camels seemed to be resistant to BLV infection. This study contributes to the nationwide effort to obtain baseline information on the prevalence of BLV, which will assist in planning the control strategy for the disease in Iran.

Download Full-text