Molecular detection of serotype groups of Listeria monocytogenes isolated from gallbladder of cattle and sheep in Iraq

Abstract The 3M™ Molecular Detection Assay (MDA) 2 – Listeria monocytogenes uses loop-mediated isothermal amplification of unique DNA target sequences combined with bioluminescence to rapidly detect Listeria monocytogenes in a broad range of food types and on environmental surfaces. Using an unpaired study design, technicians from 13 laboratories located in the United States and Canada compared the 3M MDA 2 – Listeria monocytogenes to the U.S. Department of Agriculture Food Safety Inspection Service Microbiology Laboratory Guidebook Chapter 8.09 “Isolation and Identification of Listeria monocytogenes from Red Meat, Poultry, and Egg Products, and Environmental Samples” reference method for the detection of L. monocytogenes in deli turkey and raw chicken breast fillet. Each matrix was evaluated at three levels of contamination: an uninoculated control level (0 CFU/test portion), a low inoculum level (0.2–2 CFU/test portion), and a high inoculum level (2–5 CFU/test portion). Statistical analysis was conducted according to the probability of detection (POD) statistical model. Results obtained for the low inoculum level test portions produced a difference in the collaborating laboratory POD (dLPOD) value of 0.04 with a 95% confidence interval of (−0.08, 0.17) for deli turkey, indicating that the difference between methods was not statistically significant at the 0.05 probability level. For raw chicken breast fillet, a dLPOD value of 0.16 with a 95% confidence interval of (0.04, 0.28) indicated a statistically significant difference, with an observed higher proportion of positive results by the candidate method compared to the reference method.

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Identification and Phylogenetic Classification of Fasciola species Isolated from Sheep and Cattle by PCR-RFLP in Zabol, in Sis-tan and Baluchistan Province, Southeast Iran

Iranian Journal of Public Health ◽

10.18502/ijph.v48i5.1811 ◽

2019 ◽

Author(s):

Sedighe MIR ◽

Mansour DABIRZADEH ◽

Mohammad Bagher ROKNI ◽

Mojgan ARYAEIPOUR ◽

Mahdi KHOSHSIMA SHAHRAKI ◽

...

Keyword(s):

Molecular Detection ◽

Fragment Length Polymorphism ◽

Its1 Region ◽

Fasciola Species ◽

Molecular Features ◽

Geographical Regions ◽

Pcr Rflp ◽

Health Policymaking ◽

Cattle And Sheep

Background: The detection of Fasciola species in various geographical regions is essential for health policymaking. Here, we aimed to identify livestock (cattle and sheep) related Fasciola genotypes by restriction fragment length polymorphism PCR. Methods: Seventy adult Fasciola flukes were collected from 70 infected livers of 35 cattle and 35 sheep slaughtered in Zabol abattoir, outh-east Iran (Jan-Jul 2017). Fasciola species were determined based on molecular features. For molecular detection, Fasciola ITS1 region was amplified and sequenced. A 700 bp fragment was amplified. These were digested with RasΙ enzyme. F. hepatica specific fragments were 47, 59, 68, 104, and 370, while those related to F. gigantica had 45, 55, 170, 370. Results: The two main species of F. hepatica and F. gigantica are responsible for fasciolosis in sheep and cattle in our region. From 35 Fasciola isolated from cattle, 3 and 32 were F. hepatica and F. giagantica respectively. From 35 Fasciola isolated from sheep, 4 were F. hepatica and 31 were F. gigantica. Conclusion: All Seventy Fasciola samples from two different hosts (cattle and sheep) were identified as either F. hepatica or F. gigantica by PCR-RFLP. Genotypic variability of Fasciola species was high in our region. It is recommended to assess molecular variation of Fasciola isolates in other host livestock.

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Isolation and Molecular Detection of Listeria monocytogenes in Minced Meat, Frozen Chicken and Cheese in Duhok Province, Kurdistan Region of Iraq

10.4172/2476-2059.1000118 ◽

2017 ◽

Vol 02 (01) ◽

Cited By ~ 1

Author(s):

Saman Said Taha Said Ahmed ◽

Bizhar Ahmed Tayeb

Keyword(s):

Listeria Monocytogenes ◽

Molecular Detection ◽

Kurdistan Region ◽

Minced Meat

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Preparation of Listeria monocytogenes specimens for molecular detection and identification

International Journal of Food Microbiology ◽

10.1016/j.ijfoodmicro.2007.11.066 ◽

2008 ◽

Vol 122 (3) ◽

pp. 229-242 ◽

Cited By ~ 24

Author(s):

Dongyou Liu

Keyword(s):

Listeria Monocytogenes ◽

Molecular Detection ◽

Detection And Identification

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An insight into the isolation, enumeration, and molecular detection of Listeria monocytogenes in food

Frontiers in Microbiology ◽

10.3389/fmicb.2015.01227 ◽

2015 ◽

Vol 6 ◽

Cited By ~ 33

Author(s):

Jodi Woan-Fei Law ◽

Nurul-Syakima Ab Mutalib ◽

Kok-Gan Chan ◽

Learn-Han Lee

Keyword(s):

Listeria Monocytogenes ◽

Molecular Detection ◽

Insight Into

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Evaluation of 3M™ Molecular Detection Assay (MDA) Listeria monocytogenes for the Detection of Listeria monocytogenes in Selected Foods and Environmental Surfaces: Collaborative Study, First Action 2014.07

Journal of AOAC International ◽

10.5740/jaoacint.15-031 ◽

2015 ◽

Vol 98 (4) ◽

pp. 980-992

Author(s):

Patrick Bird ◽

Jonathan Flannery ◽

Erin Crowley ◽

James Agin ◽

David Goins ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Environmental Samples ◽

Molecular Detection ◽

Collaborative Study ◽

Probability Of Detection ◽

Cottage Cheese ◽

Inoculum Level ◽

Test Portion ◽

Detection Assay ◽

Stressed Cells

Abstract The 3M™ Molecular Detection Assay (MDA) Listeria monocytogenes combines isothermal amplification and bioluminescence to detect Listeria monocytogenes with high specificity and efficiency in select foods and environmental samples. The 3M MDA Listeria monocytogenes method was evaluated using an unpaired study design in a multilaboratory collaborative study to the U.S. Department of Agriculture, Food Safety and Inspection Service Microbiology Laboratory Guidebook 8.09 (2011) Isolation and Identification of Listeria monocytogenes from Red Meat, Poultry, and Egg Products and Environmental Samples for deli turkey, and the AOAC Official Method of AnalysisSM 993.12 Listeria monocytogenes in Milk and Dairy Products for full-fat (4% milk fat) cottage cheese following the current AOAC guidelines. A total of 16 laboratories located in the continental United States and Canada participated in this collaborative study. For deli turkey, 125 g test portions were evaluated using heat-stressed cells by each method. For full-fat cottage cheese, 25 g test portions were evaluated using nonheat-stressed cells. Each matrix had three inoculation levels: an uninoculated control level (0 CFU/test portion), and two levels artificially contaminated with L. monocytogenes, a low inoculum level (0.2–2 CFU/test portion) and a high inoculum level (2–5 CFU/test portion). In total, 1584 unpaired replicate samples were analyzed. Statistical analysis was conducted according to the probability of detection (POD) model. Results obtained for the low inoculum level full-fat cottage cheese test portions produced a difference in cross-laboratory POD (dLPOD) value of –0.08 with a 95% confidence interval (CI) of (–0.20, 0.05). For the low-level deli turkey test portions, a dLPOD value of –0.02 with a 95% CI of (–0.14, 0.11) was obtained.

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