Molecular detection of Rift Valley fever virus in serum samples from selected areas of Tanzania

2014 ◽  
Vol 46 (4) ◽  
pp. 629-634 ◽  
Author(s):  
Augustino Alfred Chengula ◽  
Christopher Jacob Kasanga ◽  
Robinson Hammerthon Mdegela ◽  
Raphael Sallu ◽  
Mmeta Yongolo
Keyword(s):  
Rift Valley ◽  
Rift Valley Fever ◽  
Molecular Detection ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Serum Samples ◽  
Valley Fever

scholarly journals Investigation of Rift Valley Fever Virus Infection Serologically and Pathologically in Aborted Cattle, Sheep, Goats and in Fetuses

2020 ◽  
pp. 1-6
Author(s):  
Mehmet Kale ◽  
Sibel Hasircioglu ◽  
Özlem Özmen ◽  
Nuri Mamak ◽  
Sibel Gür ◽  
...  
Keyword(s):  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Western Mediterranean ◽  
Fever Virus ◽  
Pathological Findings ◽  
Elisa Method ◽  
Serum Samples ◽  
Valley Fever ◽  
Sheep And Goats

In this study, Rift Valley Fever Virus (RVFV) infection was searched serologically and pathologically in cattle (178 Holstein), sheep (160 native), goats (66 ordinary goats, 98 Honamli goats, 16 Saanen goats) with an abortion history and in unborn cattle (8), sheep (24) and goat (5) fetus. Samples were collected between July 2009 and September 2010. As a result of studying specific antibodies to RVFV by using the c-ELISA method in blood serum samples collected from cattle, sheep and goats suffering abortion, seropositivity was identified in 7 cattle (7/178; 3.93%), 4 sheep (4/160;2.50%) and 18 goats (18/180;10.0%). 18 seropositive goats were distributed according to race as 13 ordinary goats (19.70%), 2 Honamli goats (2.04%) and 3 Saanen goats (18.75%). When liver, spleen and brain samples of the unborn fetus of cattle, sheep and goats were studied histopathologically, no pathological findings on RVFV disease were obtained. Consequently, in this study, where RVFV infection in cattle, sheep and goats raised in Western Mediterranean Region of Turkey was serologically revealed, it was concluded that RVFV did not take place in the aetiology of abortion cases in relevant species. 

scholarly journals International External Quality Assessment of Molecular Detection of Rift Valley Fever Virus

2013 ◽  
Vol 7 (5) ◽  
pp. e2244 ◽  
Author(s):  
Camille Escadafal ◽  
Janusz T. Paweska ◽  
Antoinette Grobbelaar ◽  
Chantel le Roux ◽  
Michèle Bouloy ◽  
...  
Keyword(s):  
Quality Assessment ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Molecular Detection ◽  
External Quality Assessment ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
External Quality ◽  
Valley Fever

scholarly journals Serological and genomic evidence of Rift Valley fever virus during inter-epidemic periods in Mauritania

2016 ◽  
Vol 145 (5) ◽  
pp. 1058-1068 ◽  
Author(s):  
M. RISSMANN ◽  
M. EIDEN ◽  
B. O. EL MAMY ◽  
K. ISSELMOU ◽  
B. DOUMBIA ◽  
...  
Keyword(s):  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Simultaneous Detection ◽  
Small Ruminants ◽  
Fever Virus ◽  
Serum Samples ◽  
Valley Fever ◽  
Epidemic Period ◽  
And Shifts

SUMMARYRift Valley fever virus (RVFV) is an emerging pathogen of major concern throughout Africa and the Arabian Peninsula, affecting both livestock and humans. In the past recurrent epidemics were reported in Mauritania and studies focused on the analysis of samples from affected populations during acute outbreaks. To verify characteristics and presence of RVFV during non-epidemic periods we implemented a multi-stage serological and molecular analysis. Serum samples of small ruminants, cattle and camels were obtained from Mauritania during an inter-epidemic period in 2012–2013. This paper presents a comparative analysis of potential variations and shifts of antibody presence and the capability of inter-epidemic infections in Mauritanian livestock. We observed distinct serological differences between tested species (seroprevalence: small ruminants 3·8%, cattle 15·4%, camels 32·0%). In one single bovine from Nouakchott, a recent RVF infection could be identified by the simultaneous detection of IgM antibodies and viral RNA. This study indicates the occurrence of a low-level enzootic RVFV circulation in livestock in Mauritania. Moreover, results indicate that small ruminants can preferably act as sentinels for RVF surveillance.

scholarly journals Co-circulation of Orthobunyaviruses and Rift Valley Fever Virus in Mauritania, 2015

2021 ◽  
Vol 12 ◽  
Author(s):  
Nicole Cichon ◽  
Yahya Barry ◽  
Franziska Stoek ◽  
Abdellah Diambar ◽  
Aliou Ba ◽  
...  
Keyword(s):  
Rift Valley ◽  
High Performance ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Small Ruminants ◽  
Hemorrhagic Fever ◽  
Cross Reactivity ◽  
Fever Virus ◽  
Serum Samples ◽  
Valley Fever

Ngari virus (NRIV) has been mostly detected during concurrent outbreaks of Rift Valley fever virus (RVFV). NRIV is grouped in the genus Orthobunyavirus within the Bunyaviridae family and RVFV in the genus Phlebovirus in the family Phenuiviridae. Both are zoonotic arboviruses and can induce hemorrhagic fever displaying the same clinical picture in humans and small ruminants. To investigate if NRIV and its parental viruses, Bunyamwera virus (BUNV) and Batai virus (BATV), played a role during the Mauritanian RVF outbreak in 2015/16, we analyzed serum samples of sheep and goats from central and southern regions in Mauritania by quantitative real-time RT-PCR, serum neutralization test (SNT) and ELISA. 41 of 458 samples exhibited neutralizing reactivity against NRIV, nine against BATV and three against BUNV. Moreover, complete virus genomes from BUNV could be recovered from two sheep as well as two NRIV isolates from a goat and a sheep. No RVFV-derived viral RNA was detected, but 81 seropositive animals including 22 IgM-positive individuals were found. Of these specimens, 61 samples revealed antibodies against RVFV and at least against one of the three orthobunyaviruses. An indirect ELISA based on NRIV/BATV and BUNV derived Gc protein was established as complement to SNT, which showed high performance regarding NRIV, but decreased sensitivity and specificity regarding BATV and BUNV. Moreover, we observed high cross-reactivity among NRIV and BATV serological assays. Taken together, the data indicate the co-circulation of at least BUNV and NRIV in the Mauritanian sheep and goat populations.

Complement fixation reaction of Rift Valley fever virus

1950 ◽  
Vol 5 (5) ◽  
pp. 243-247
Author(s):  
Minoru MATSUMOTO ◽  
Saburo IWASA ◽  
Motosige ENDO
Keyword(s):  
Rift Valley ◽  
Rift Valley Fever ◽  
Complement Fixation ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever ◽  
Fixation Reaction

scholarly journals The Use of Nanotrap Particles in the Enhanced Detection of Rift Valley Fever Virus Nucleoprotein

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0128215 ◽  
Author(s):  
Nazly Shafagati ◽  
Lindsay Lundberg ◽  
Alan Baer ◽  
Alexis Patanarut ◽  
Katherine Fite ◽  
...  
Keyword(s):  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever

scholarly journals Production of small ruminant morbillivirus, rift valley fever virus and lumpy skin disease virus in CelCradle™ -500A bioreactors

2021 ◽  
Vol 17 (1) ◽  
Author(s):  
Halima Rhazi ◽  
Najete Safini ◽  
Karima Mikou ◽  
Meryeme Alhyane ◽  
Khalid Omari Tadlaoui ◽  
...  
Keyword(s):  
Disease Virus ◽  
Skin Disease ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever ◽  
Lumpy Skin Disease ◽  
Lumpy Skin Disease Virus ◽  
Cell Factories

Abstract Background Animal vaccination is an important way to stop the spread of diseases causing immense damage to livestock and economic losses and the potential transmission to humans. Therefore effective method for vaccine production using simple and inexpensive bioprocessing solutions is very essential. Conventional culture systems currently in use, tend to be uneconomic in terms of labor and time involved. Besides, they offer a limited surface area for growth of cells. In this study, the CelCradle™-500A was evaluated as an alternative to replace conventional culture systems in use such as Cell factories for the production of viral vaccines against small ruminant morbillivirus (PPR), rift valley fever virus (RVF) and lumpy skin disease virus (LSD). Results Two types of cells Vero and primary Lamb Testis cells were used to produce these viruses. The study was done in 2 phases as a) optimization of cell growth and b) virus cultivation. Vero cells could be grown to significantly higher cell densities of 3.04 × 109 using the CelCradle™-500A with a shorter doubling time as compared to 9.45 × 108 cells in Cell factories. This represents a 19 fold increase in cell numbers as compared to seeding vs only 3.7 fold in Cell factories. LT cells achieved modestly higher cell densities of 6.7 × 108 as compared to 6.3 × 108 in Cell factories. The fold change in densities for these cells was 3 fold in the CelCradle™-500A vs 2.5 fold in Cell factories. The titers in the conventional system and the bioreactor were not significantly different. However, the Cell-specific virus yield for rift valley fever virus and lumpy skin disease virus are higher (25 virions/cell for rift valley fever virus, and 21.9 virions/cell for lumpy skin disease virus versus 19.9 virions/cell for rift valley fever virus and 10 virions/cell for lumpy skin disease virus). Conclusions This work represents a novel study for primary lamb testis cell culture in CellCradle™-500A bioreactors. In addition, on account of the high cell densities obtained and the linear scalability the titers could be further optimized using other culture process such us perfusion.

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