First report on molecular characterization and phylogenetic analysis of Reticuloendotheliosis virus in Sudan

2020 ◽  
Vol 52 (4) ◽  
pp. 2073-2078 ◽  
Author(s):  
Shima H. Alfaki ◽  
Mohammed O. Hussien ◽  
Nussieba A. Osman ◽  
Khalid A. Enan ◽  
Abdel Rahim M. El Hussein
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Reticuloendotheliosis Virus ◽  
First Report

First report of molecular characterization and phylogenetic analysis of Sarcocystis tenella from India

2019 ◽  
Vol 118 (5) ◽  
pp. 1429-1434 ◽  
Author(s):  
Vikrant Sudan ◽  
Rahul Kumar ◽  
Daya Shanker ◽  
Sanjhi Paliwal
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
First Report ◽  
Sarcocystis Tenella

First report of molecular characterization and sequence phylogenetic analysis of Linguatula serrata from India

2018 ◽  
Vol 63 (4) ◽  
pp. 781-783 ◽  
Author(s):  
Vikrant Sudan ◽  
Daya Shanker ◽  
Amit Kumar Jaiswal
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
The Other ◽  
Mesenteric Lymph Nodes ◽  
Linguatula Serrata ◽  
First Report ◽  
Mesenteric Lymph ◽  
Indian Isolates ◽  
I Gene

Abstract Linguatula serrata is a pentastomid which is worldwide in distribution. However, a very few references are reported from India. In the present study, the cox I gene of L. serrata nymphs, originally isolated from mesenteric lymph nodes of buffaloes, was amplified and custom sequenced. Based on sequence analysis, two haplotypes were noticed and were subsequently submitted in NCBI database. The sequences were also compared with the other sequences available in the pubmed and phylogenetic analysis coupled with nucleotide homologies were commutated. The studied Indian isolates were found closer to Bangladesh and Iran isolates. This is the first report of molecular characterization of L. serrata from India.

Morphological, histological and molecular characterization of Myxidium cf. rhodei infecting the kidney of Rutilus rutilus

2020 ◽  
Vol 141 ◽  
pp. 39-46
Author(s):  
MD Dorjievna Batueva ◽  
X Pan ◽  
J Zhang ◽  
X Liu ◽  
W Wei ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Rutilus Rutilus ◽  
Longitudinal Axis ◽  
Suture Line ◽  
Present Species ◽  
Supplementary Data

In the present study, we provide supplementary data for Myxidium cf. rhodei Léger, 1905 based on morphological, histological and molecular characterization. M. cf. rhodei was observed in the kidneys of 918 out of 942 (97%) roach Rutilus rutilus (Linnaeus, 1758). Myxospores of M. cf. rhodei were fusiform with pointed ends, measuring 12.7 ± 0.1 SD (11.8-13.4) µm in length and 4.6 ± 0.1 (3.8-5.4) µm in width. Two similar pear-shaped polar capsules were positioned at either ends of the longitudinal axis of the myxospore: each of these capsules measured 4.0 ± 0.1 (3.1-4.7) µm in length and 2.8 ± 0.1 (2.0-4.0) µm in width. Polar filaments were coiled into 4 to 5 turns. Approximately 18-20 longitudinal straight ridges were observed on the myxospore surface. The suture line was straight and distinctive, running near the middle of the valves. Histologically, the plasmodia of the present species were found in the Bowman’s capsules, and rarely in the interstitium of the host. Phylogenetic analysis revealed that M. cf. rhodei was sister to M. anatidum in the Myxidium clade including most Myxidium species from freshwater hosts.

Molecular characterization and first report ofCryptosporidiumgenotypes in human population in the Slovak Republic

2015 ◽  
Vol 36 (23) ◽  
pp. 2925-2930 ◽  
Author(s):  
Antónia Petrincová ◽  
Alexandra Valenčáková ◽  
Lenka Luptáková ◽  
František Ondriska ◽  
Jana Kalinová ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Human Population ◽  
Slovak Republic ◽  
First Report

Molecular Detection and Phylogenetic Analysis of Chicken Astrovirus Associated with Poultry Enteritis

2021 ◽  
Author(s):  
C. Patidar ◽  
D.K. Sharma ◽  
R. Singathia ◽  
P. Suthar ◽  
A. Saraswat ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Molecular Characterization ◽  
Histopathological Examination ◽  
Villous Atrophy ◽  
Inflammatory Cells ◽  
Liver Parenchyma ◽  
Amino Acid Sequences ◽  
Poultry Birds ◽  
Commercial Poultry ◽  
Liver Specimen

Background: Poultry enteritis is an important multifactorial disease. Chicken Astrovirus (CAstV) usually associated with enteritis. The aim of this study was to investigate the occurrence of CAstV in poultry enteritis cases, its molecular characterization, phylogenetic analysis and gross and microscopic examination of intestine and liver specimen affected with CAstV. Methods: Total 604 dead poultry birds from commercial poultry farms affected with enteritis were examined for presence of CAstV. Intestinal samples of four birds were pooled to make one biological sample. CAstV was detected by reverse transcriptase PCR (RT-PCR) using ORF-1b gene specific primers. Molecular characterization was carried out by partial gene sequencing. Result: CAstV was detected in 20.52% (31/151) of samples. Highest prevalence (49.29%) was observed in 0-1 week old chicks. The partial molecular characterization revealed high similarity of the nucleotide sequence from India (97% to 93%) and from USA, Brazil, Poland and Korea (94 to 92%). Further similarity of amino acid sequences of CAstV from India (100% to 98%) and from USA, Brazil, Poland and Korea (98 to 97%) was observed. Histopathological examination revealed villous atrophy, congestion and atrophic cystic glands in sub-mucosa of intestine. Further severe congestion and hemorrhages along with infiltration of inflammatory cells in liver parenchyma was observed.

Sign in / Sign up

Export Citation Format

Share Document