ULTRASTRUCTURAL CHANGES OF THE MESENTRIC LYMPH NODES OF CALVES EXPERIMENTALLY INFECTED WITH SALMONELLA TYPHIMURIUM

A systemic study was done on the pathogenesis of experimentally induced Salmonella typhimurium infection in calves. The present investigation was carried out on sixteen normal colostrum fed friesian calves, ranging in age from 3 to 6 weeks. The calves were divided into two equal groups. Group I inoculated orally with (1.5 x 10'') Salmonella typhimurium and group IA served as control. • The early ultrastructural alteration in the mesenteric lymph nodes was the presence of many free Salmonella in localized vacuoles. The interaction between the host cells and phagocytized Salmonella was also observed.

Evaluation of Nonviral piggyBac and lentiviral Vector in Functions of CD19chimeric Antigen Receptor T Cells and Their Antitumor Activity for CD19+ Tumor Cells

Nonviral transposon piggyBac (PB) and lentiviral (LV) vectors have been used to deliver chimeric antigen receptor (CAR) to T cells. To understand the differences in the effects of PB and LV on CAR T-cell functions, a CAR targeting CD19 was cloned into PB and LV vectors, and the resulting pbCAR and lvCAR were delivered to T cells to generate CD19pbCAR and CD19lvCAR T cells. Both CD19CAR T-cell types were strongly cytotoxic and secreted high IFN-γ levels when incubated with Raji cells. TNF-α increased in CD19pbCAR T cells, whereas IL-10 increased in CD19lvCAR T cells. CD19pbCAR and CD19lvCAR T cells showed similar strong anti-tumor activity in Raji cell-induced mouse models, slightly reducing mouse weight while enhancing mouse survival. High, but not low or moderate, concentrations of CD19pbCAR T cells significantly inhibited Raji cell-induced tumor growth in vivo. These CD19pbCAR T cells were distributed mostly in mesenteric lymph nodes, bone marrow of the femur, spleen, kidneys, and lungs, specifically accumulating at CD19-rich sites and CD19-positive tumors, with CAR copy number being increased on day 7. These results indicate that pbCAR has its specific activities and functions in pbCAR T cells, making it a valuable tool for CAR T-cell immunotherapy.

Prevalence and antimicrobial resistance of Salmonella spp. isolated from free-ranging wild boars in the State of São Paulo, Brazil

ABSTRACT: European wild boars (Sus scrofa) are considered exotic invasive species worldwide. Invasions of wild boars are a growing public health concern, as wild boars may represent an important reservoir of zoonotic pathogens, including bacteria of the genus Salmonella. The aim of this study was to determine the prevalence and serovars of Salmonella spp. in free-ranging wild boars legally hunted in the state of São Paulo, Brazil, and the susceptibility of those Salmonella spp. to antimicrobials. Fecal samples and mesenteric lymph nodes were acquired from 63 wild boars. The prevalence of Salmonella spp. in free-ranging wild boars was 9.5 % (6/63; confidence interval: 4.4 % - 19.2 %). Six serovars were isolated: S. enterica subsp. enterica ser. 4,5,12:-:1,2, S. enterica ser. Cerro, S. enterica ser. Madelia, S. enterica ser. Typhimurium, S. enterica ser. I (4,5,12:i:-) and S. enterica ser. Muenster. Analysis of antimicrobial resistance of Salmonella spp. showed that the majority of serovars were fully susceptible to the tested antimicrobials. Only S. enterica ser. Typhimurium and S. enterica ser. Muenster showed a resistance pattern to at least one antimicrobial analyzed. To our knowledge, this study is the first report the prevalence and serovars of Salmonella spp. in free-ranging wild boars in the State of São Paulo, Brazil. Results indicate a low prevalence with variability of Salmonella serovars, with some pattern of antimicrobial resistance. This study highlights the potential role of wild boars as carriers of Salmonella and could pose a risk to wild and domestic animals as well as humans.

Pathomorphological changes in the early postnatal period in a calf with abomasum rupture

The main cause of the disease and death of calves in the early postembryonic period of development is a feeding disorder. The aim of the work was to analyze macro-and micromorphological changes in the digestive system of a calf with a rennet rupture. To achieve this goal, standard macro-and microscopic research methods were used. Macroscopic examination revealed thickening, erosion of the mucous membrane of the esophageal gutter rollers, accumulation of contents in the scar, mesh and book, hyperemia of the mucous membranes; thinning and rupture of the rennet wall; pronounced hyperemia of the mucous membrane of the small intestine, thickening of the mucous membrane of the thick section; an increase in mesenteric lymph nodes and a change in their consistency. Microscopically, the thickness of the epithelium, submucosal and muscle layer is most developed in the abomasum and was, respectively, 0.68-0.72 mm, 0.23-0.32 mm and 0.98-1.05 mm. Villi were found in the folds of the scar, numerous well – developed longitudinally oriented protrusions were found in the book, lymphocellular clusters and bottom glands were found in the rennet; folds with well – developed crypts were found in the colon. Thus, as a result of feeding coarse feed, the deceased calf has catarrhal erosive lesions of the esophageal trough; serous-catarrhal abomasitis with signs of hemorrhagic with dilation and perforation in the cardiac part; hemorrhagic omasitis against the background of a book blockage; catarrhal reticulitis; catarrhal ruminitis and catarrhal enterocolitis.

Scars of hope and forewarn of a bleak future: Post-mortem findings of dugongs (Dugong dugon) belonging to a relict population in the Gulf of Kachchh, India

A relict dugong population resides in the Gulf of Kachchh in Gujarat state of India. Very little is known on this population stemming from a lack of focused studies and inadequate examinations of previous strandings. In this study, crucial ecological information is gathered through a systematic necropsy on stranded dugongs in the gulf. As indicated through dorsal tusk-rake scars on both the carcasses, this study presents the first records of derivative physical evidence to the presence of a socially interacting population. Progressive healing and differences in the width of the scars indicated more than two individuals had participated in a sexual agnostic or courtship event. Conversely, our findings report that both the animals suffered relative effects of asphyxiation after fishing net entanglement. An implication of a prior pathological condition(s) in the form of dermal cysts, swollen mesenteric lymph nodes, and endoparasites are also reported. Stomach content was examined for a qualitative dietary characterization. Other potential threats along with fishing net microfilaments found in the stomach contents of both dugongs are discussed in brief.

Intestinal helminth infection transforms the CD4+ T cell composition of the skin

Intestinal helminth parasites can alter immune responses to vaccines, other infections, allergens and autoantigens, implying effects on host immune responses in distal barrier tissues. We herein show that the skin of C57BL/6 mice infected with the strictly intestinal nematode Heligmosomoides polygyrus contain higher numbers of CD4+ T cells compared to the skin of uninfected controls. Accumulated CD4+ T cells were H. polygyrus-specific TH2 cells that skewed the skin CD4+ T cell composition towards a higher TH2/TH1 ratio which persisted after worm expulsion. Accumulation of TH2 cells in the skin was associated with increased expression of the skin-homing chemokine receptors CCR4 and CCR10 on CD4+ T cells in the blood and mesenteric lymph nodes draining the infected intestine and was abolished by FTY720 treatment during infection, indicating gut-to-skin trafficking of cells. Remarkably, skin TH2 accumulation was associated with impaired capacity to initiate IFN-γ recall responses and develop skin-resident memory cells to mycobacterial antigens, both during infection and months after deworming therapy. In conclusion, we show that infection by a strictly intestinal helminth has long-term effects on immune cell composition and local immune responses to unrelated antigens in the skin, revealing a novel process for T cell colonisation and worm-mediated immunosuppression in this organ.

INFLUENCE OF DIFFERENT DOSES OF TISSUE BIOSTIMULANT ON BIOCHEMICAL BLOOD INDICES OF YOUNG CATTLE

Изучено влияние введения разных доз тканевого биостимулятора на биохимические показатели сыворотки крови ремонтного молодняка крупного рогатого скота. Опыт проводился в Алтайском крае на 4 группах ремонтных телочек Приобского типа черно-пестрой породы по 10 голов в каждой. При подборе животных учитывались возраст (1 мес) и живая масса (51,3±1,48 кг). Продолжительность опыта составляла 14 дней. Животным контрольной группы подкожно однократно вводили физиологический раствор в дозе 3,0 мл на 1 голову, I опытной — тканевый биостимулятор в дозе 2,0 мл, II — 3,0 мл, III — 4,0 мл на 1 голову. Опытную партию тканевого биостимулятора изготовили из субпродуктов и боенских отходов пантовых оленей по запатентованной технологии. Материалом для приготовления препарата служили мезентеральные лимфоузлы и средостения, селезенка, печень, матки с плодами (2—3 мес), плацента, отобранные в асептических условиях во время убоя здоровых животных. Введение тканевого биостимулятора телочкам в разных дозах способствовало повышению некоторых исследуемых биохимических показателей сыворотки крови. Наиболее оптимальной дозой применения тканевого биостимулятора следует считать 3,0 мл/гол, что способствует повышению содержания общего белка в сыворотке крови на 1,4% (P≤0,05), глюкозы — на 22,6% (P≤0,05) и снижению содержания холестерина на 12,3% (P≤0,05). The effect of the administration of different doses of the tissue biostimulant on the biochemical blood serum indices of replacement young cattle was studied. The experiment was carried out in the Altai Region in 4 groups of 10 Black-Pied replacement heifers of the Priobskiy type. When selecting the animals, their age (1 month) and live weight (51.3±1.48 kg) was taken into account. The experiment lasted 14 days. Saline solution was injected under the skin to the animals of the control group at a dose of 3.0 mL per 1 head; the tissue bio-stimulant was administered in the following doses: in the 1st trial group — 2.0 mL per head; the 2nd trial group 3.0 mL per head; the 3rd trial group — 4.0 mL per 1 head. The trial batch of the tissue bio-stimulant was made from velvet antler deer by-products and slaughterhouse offal by using the patent-protected technology. The tissue biostimulant was made from mesenteric lymph nodes and mediastinums, spleen, liver, uteri with 2—3 month old fetuses, and placentae collected under aseptic conditions from healthy animals at slaughter. The administration of the tissue bio-stimulant to heifers in different doses increased some of the studied biochemical blood serum indices. The tissue biostimulant dose of 3.0 mL per head should be considered the most optimal one; it increased the total protein content in blood serum by 1.4% (P≤0.05), glucose — by 22.6% (P≤0.05), and decreased cholesterol content by 12.3% (P≤0.05).

FEATURES OF ULTRASONIC CHARACTERISTICS OF MESENTERIC LYMPH NODES IN COVID-19 IN CHILDREN WITH ACUTE ABDOMINAL SYNDROME: DESCRIPTION OF CLINICAL CASES

The authors describe clinical observations of children with acute mesenteric adenitis with PCRconfirmed COVID-19 infection. Attention during ultrasound diagnostics is drawn to the condition of the lymph nodes – mesenteric adenitis in COVID-19 infection was manifested by a significant increase in the size of the nodes – more than 15 mm, a large number of nodes in the cut with a tendency to form a conglomerate, a more rounded shape, significantly increased blood flow, a reaction of the peritoneum in the form its thickening and the presence of free fluid in the abdominal cavity. Unusual for a «ordinary» respiratory viral or intestinal infection type of lymph nodes was the only sign that distinguishes the manifestations of mesenteric infection in COVID-19 infection.

Immune Evaluation of Recombinant Lactobacillus plantarum With Surface Display of HA1-DCpep in Mice

Avian influenza viruses can be efficiently transmitted through mucous membranes, and conventional vaccines are not effective in protecting against mucosal infection by influenza viruses. To induce multiple immune responses in an organism, we constructed a recombinant Lactobacillus plantarum expressing the influenza virus antigen HA1 with the adjuvant dendritic cell-targeting peptide (DCpep). The recombinant L. plantarum strains NC8Δ-pWCF-HA1 and NC8Δ-pWCF-HA1-DCpep were used to immunize mice via oral administration, and the humoral, cellular and mucosal immune responses were evaluated. In addition, the serum levels of specific antibodies and hemagglutination inhibition (HI) levels were also measured. Our results showed that recombinant L. plantarum activated dendritic cells in Peyer’s patches (PPs), increased the numbers of CD4+IFN-γ+ and CD8+IFN-γ+ cells in the spleen and mesenteric lymph nodes (MLNs), and affected the ability of CD4+ and CD8+ cells to proliferate in the spleen and MLNs. Additionally, recombinant L. plantarum increased the number of B220+IgA+ cells in PPs and the level of IgA in the lungs and different intestinal segments. In addition, specific IgG, IgG1 and IgG2a antibodies were induced at high levels in the mice serum, specific IgA antibodies were induced at high levels in the mice feces, and HI potency was significantly increased. Thus, the recombinant L. plantarum strains NC8Δ-pWCF-HA1 and NC8Δ-pWCF-HA1-DCpep have potential as vaccine candidates for avian influenza virus.

Mitotically active cellular fibroma of the ovary diagnosis and treatment

Mitotically active cellular fibroma (MACF) of the ovary is a relatively newly recognized histological disease entity which explains its scarce reporting in literature and unknown prognosis [1]. In this tumor type, high mitotic activity is noticed without severe atypia. These tumors are categorized as benign ovarian tumors with late recurrence, if any. However, we herein report an unusual case of an ovarian mitotically active atypical fibrous tumor case which presented as recurrence within six months of being operated thereby raising suspicion of an element of malignancy. On presentation, the patient suffered from abdominal pain and abdomino-pelvic mass. After imaging confirmed an ovarian neoplastic lesion patient underwent bilateral salpingo-oophorectomy and hysterectomy. Histopathology defined the lesion as “ovarian mitotically active atypical fibrous tumor”. Six months later, abdominal pain recurred for which imagining was done and confirmed a newly developed well defined irregular complex solid cystic mass causing intestinal obstruction giving an impression of a malignant neoplasm, probably ovarian carcinoma. However, histopathology for the new lesion remained the same as was for the previous mass, i.e. mitotically active ovarian fibroma, but with lung and mesenteric lymph nodes metastases. Patient couldn’t be operated on due to her co-morbid conditions, and the mass was vascular. She was given supportive care with consideration to start hormonal therapy and during this period died due to sepsis. This case highlights the importance of having a high suspicion of the possibility of malignancy when mitotically active cellular fibroma manifests as a recurrence of mass as this tumor may tend to have an uncertain malignant potential. Thereby, stressing the importance of long term follow up even after the primary tumor is surgically treated without any surgical difficulty or tumor rupture [2]. Such tumors need to be followed and reported more often to help understanding the way they behave and their management.