Characterization of three new S-alleles and development of an S-allele-specific PCR system for rapidly identifying the S-genotype in apple cultivars

2009 ◽  
Vol 6 (2) ◽  
pp. 161-168 ◽  
Author(s):  
Shenshan Long ◽  
Maofu Li ◽  
Zhenhai Han ◽  
Kun Wang ◽  
Tianzhong Li
Keyword(s):  
Specific Pcr ◽  
S Alleles ◽  
Allele Specific ◽  
Apple Cultivars ◽  
S Allele ◽  
Allele Specific Pcr

scholarly journals Update on and Review of the Incompatibility (S-) Genotypes of Apple Cultivars

HortScience ◽  
2004 ◽  
Vol 39 (5) ◽  
pp. 943-947 ◽  
Author(s):  
Wim Broothaerts ◽  
Ilse Van Nerum ◽  
Johan Keulemans
Keyword(s):  
Identification System ◽  
Published Data ◽  
Specific Pcr ◽  
S Alleles ◽  
Allele Specific ◽  
Self Fertilization ◽  
Apple Cultivars ◽  
S Allele ◽  
Allele Specific Pcr

Apple cultivars display a self-incompatibility system that restricts self-fertilization and fertilization between cultivars bearing identical S-alleles. There has been considerable progress in identification of S-alleles in apple in recent years and methods are now available for the accurate S-genotyping of cultivars. Following a recently revised numerical identification system for apple S-alleles, we present the first extensive compilation of apple cultivars with their S-genotypes. This list contains data from our own investigations using S-allele-specific PCR methodology, including a number of new data, as well as published data from various other sources. Eighteen different S-alleles are discriminated, which allowed the determination of the S-genotypes for 150 diploid or triploid European, American, and Japanese cultivars. Many of these cultivars are cultivated worldwide for their fruit. Also included are a number of old, obsolete cultivars and a few nondomestic genotypes. We observed a wide variation in the frequency of S-alleles in the apple germplasm. Three S-alleles (S2, S3, and S9) are very common in the cultivars evaluated, presumably as a result of the widespread use of the same breeding parents, and seven alleles are very rare (S4, S6, S8, S16, S22, S23, S26).

scholarly journals Combined Analysis of S-Alleles in European Pear by Pollinations and PCR-based S-Genotyping; Correlation between S-Phenotypes and S-RNase Genotypes

2008 ◽  
Vol 133 (2) ◽  
pp. 213-224 ◽  
Author(s):  
Javier Sanzol ◽  
Timothy P. Robbins
Keyword(s):  
Pollen Tube ◽  
Fruit Set ◽  
Breeding Programs ◽  
European Pear ◽  
Specific Pcr ◽  
S Alleles ◽  
Allele Specific ◽  
S Allele ◽  
Consensus Primers ◽  
Allele Specific Pcr

Pollen–pistil incompatibility in european pear (Pyrus communis L.) compromises adequate orchard pollination and fruit set and restricts cross-fertility between cultivars suitable as parents in breeding programs. Genetic control is simple, with a single locus expressed gametophytically in pollen controlling the rejection of the pollen tube in the style. Semicompatible pollination arises when only one allele of a pollen parent matches the pistil. Semicompatible test-crosses using partially S-genotyped european pear cultivars allowed the discrimination of 14 S-alleles (S1 to S14) at the phenotypic level and the assignment of 33 cultivars to 13 incompatibility groups. Partial genomic sequences of the S-RNase gene, spanning between the C1 and C5 conserved regions, were obtained for each new S-allele identified (S6 to S14). These sequences and those reported previously for the S1 to S5 RNases allowed a set of consensus primers amplifying all 14 S-RNase alleles to be designed. Allele-specific PCR allowed discrimination between those S-RNases giving amplification products of similar size with consensus primers. These two approaches provided a method for the molecular identification of all 14 S-alleles in european pear. With this methodology, we demonstrate that the S-RNase genotypes inferred from PCR exactly matches the S-phenotypes deduced from test-crosses. Comparison of the sequences obtained with those of S-RNases already published allowed us to relate S-alleles between studies. This will allow the prediction of cross-incompatibility among an even larger number of european pear cultivars.

scholarly journals Identification of Self-incompatibility Genotypes in Apple and Crabapple Cultivars by S-allele Specific PCR Analysis

2014 ◽  
Vol 46 (4) ◽  
pp. 364-371
Author(s):  
Kang Hee Cho ◽  
Jeong-Hee Kim2 ◽  
Jung Woo Lee ◽  
Soon-Il Kwon ◽  
Jong Taek Park ◽  
...  
Keyword(s):  
Pcr Analysis ◽  
Self Incompatibility ◽  
Specific Pcr ◽  
Allele Specific ◽  
S Allele ◽  
Allele Specific Pcr

A molecular method for S-allele identification in apple based on allele-specific PCR

1995 ◽  
Vol 91 (4) ◽  
pp. 691-698 ◽  
Author(s):  
G. A. Janssens ◽  
I. J. Goderis ◽  
W. F. Broekaert ◽  
W. Broothaerts
Keyword(s):  
Molecular Method ◽  
Specific Pcr ◽  
Allele Specific ◽  
S Allele ◽  
Allele Specific Pcr ◽  
Allele Identification

scholarly journals Determining the Self-incompatibility Alleles of Japanese Apple Cultivars

HortScience ◽  
1997 ◽  
Vol 32 (7) ◽  
pp. 1258-1259 ◽  
Author(s):  
Kenji Sakurai ◽  
Susan K. Brown ◽  
Norman F. Weeden
Keyword(s):  
Polymerase Chain Reaction Amplification ◽  
Unreduced Gamete ◽  
Restriction Enzyme Digestion ◽  
S Alleles ◽  
Allele Specific ◽  
Apple Cultivars ◽  
Reaction Amplification ◽  
Incompatibility Alleles ◽  
Polymerase Chain ◽  
S Allele

The S alleles of 15 Japanese apple cultivars were determined by using the allele-specific polymerase chain reaction amplification and restriction enzyme digestion system developed by Janssens et al. (1995). Both S alleles were identified in eight diploid cultivars, two S alleles in three triploid cultivars, and one S allele in the remaining four diploid cultivars. Two cultivars had S alleles different than those predicted by their parentage, and in one comparison of a cultivar with its sport, an identity problem was discovered. The technique helped to indicate the parent contributing the unreduced gamete in triploids.

scholarly journals Self-Incompatibility Alleles in Iranian Pear Cultivars

2019 ◽  
Author(s):  
Maryam Bagheri ◽  
Ahmad Ershadi
Keyword(s):  
Rapid Method ◽  
Pcr Amplification ◽  
Self Incompatibility ◽  
Specific Primers ◽  
Specific Pcr ◽  
S Alleles ◽  
Allele Specific ◽  
Incompatibility Alleles ◽  
Consensus Primers ◽  
Allele Specific Pcr

AbstractIn the present study, the S-alleles of eighteen pear cultivars, (including fourteen cultivars planted commercially in Iran and four controls) are determined. 34 out of 36 S-alleles are detected using nine allele-specific primers, which are designed for amplification of S101/S102, S105, S106, S107, S108, S109, S111, S112 and S114, as well as consensus primers, PycomC1F and PycomC5R. S104, S101 and S105 were the most common S-alleles observed, respectively, in eight, seven and six cultivars. In 16 cultivars, (‘Bartlett’ (S101S102), ‘Beurre Giffard’ (S101S106), ‘Comice’ (S104S105), ‘Doshes’ (S104S107), ‘Koshia’ (S104S108), ‘Paskolmar’ (S101S105), ‘Felestini’ (S101S107), ‘Domkaj’ (S104S120), ‘Ghousi’ (S104S107), ‘Kaftar Bache’ (S104S120), ‘Konjoni’ (S104S108), ‘Laleh’ (S105S108), ‘Natanzi’ (S104S105), ‘Sebri’ (S101S104), ‘Se Fasleh’ (S101S105) and ‘Louise Bonne’ (S101S108)) both alleles are identified but in two cultivars, (‘Pighambari’ (S105) and ‘Shah Miveh Esfahan’ (S107)) only one allele is recognized. It is concluded that allele-specific PCR amplification can be considered as an efficient and rapid method to identify S-genotype of Iranian pear cultivars.

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