Effect of Pretreatment Severity on Accumulation of Major Degradation Products from Dilute Acid Pretreated Corn Stover and Subsequent Inhibition of Enzymatic Hydrolysis of Cellulose

2012 ◽  
Vol 168 (2) ◽  
pp. 406-420 ◽  
Author(s):  
Byung-Hwan Um ◽  
G. Peter van Walsum
Keyword(s):  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
Degradation Products ◽  
Dilute Acid ◽  
Pretreated Corn Stover ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Pretreatment Severity ◽  
Hydrolysis Of

Deposition of Lignin Droplets Produced During Dilute Acid Pretreatment of Maize Stems Retards Enzymatic Hydrolysis of Cellulose

2007 ◽  
Vol 23 (6) ◽  
pp. 1333-1339 ◽  
Author(s):  
M.J. Selig ◽  
S. Viamajala ◽  
S.R. Decker ◽  
M.P. Tucker ◽  
M.E. Himmel ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Dilute Acid Pretreatment ◽  
Dilute Acid ◽  
Acid Pretreatment ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Economic impact of total solids loading on enzymatic hydrolysis of dilute acid pretreated corn stover

2010 ◽  
Vol 26 (5) ◽  
pp. 1245-1251 ◽  
Author(s):  
David Humbird ◽  
Ali Mohagheghi ◽  
Nancy Dowe ◽  
Daniel J. Schell
Keyword(s):  
Enzymatic Hydrolysis ◽  
Economic Impact ◽  
Corn Stover ◽  
Dilute Acid ◽  
Total Solids ◽  
Pretreated Corn Stover ◽  
Solids Loading ◽  
Hydrolysis Of

Strategies to achieve high-solids enzymatic hydrolysis of dilute-acid pretreated corn stover

2015 ◽  
Vol 187 ◽  
pp. 43-48 ◽  
Author(s):  
Wenhui Geng ◽  
Yongcan Jin ◽  
Hasan Jameel ◽  
Sunkyu Park
Keyword(s):  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
High Solids ◽  
Dilute Acid ◽  
Pretreated Corn Stover ◽  
Hydrolysis Of

Immobilized Nanoparticles-Mediated Enzymatic Hydrolysis of Cellulose for Clean Sugar Production: A Novel Approach

2019 ◽  
Vol 15 (3) ◽  
pp. 296-303 ◽  
Author(s):  
Swapnil Gaikwad ◽  
Avinash P. Ingle ◽  
Silvio Silverio da Silva ◽  
Mahendra Rai
Keyword(s):  
Catalytic Activity ◽  
Enzymatic Hydrolysis ◽  
Immobilized Enzyme ◽  
Free Enzyme ◽  
Magnetic Nanomaterials ◽  
Sugar Production ◽  
Cellulase Enzyme ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Background: Enzymatic hydrolysis of cellulose is an expensive approach due to the high cost of an enzyme involved in the process. The goal of the current study was to apply magnetic nanomaterials as a support for immobilization of enzyme, which helps in the repeated use of immobilized enzyme for hydrolysis to make the process cost-effective. In addition, it will also provide stability to enzyme and increase its catalytic activity. Objective: The main aim of the present study is to immobilize cellulase enzyme on Magnetic Nanoparticles (MNPs) in order to enable the enzyme to be re-used for clean sugar production from cellulose. Methods: MNPs were synthesized using chemical precipitation methods and characterized by different techniques. Further, cellulase enzyme was immobilized on MNPs and efficacy of free and immobilized cellulase for hydrolysis of cellulose was evaluated. Results: Enzymatic hydrolysis of cellulose by immobilized enzyme showed enhanced catalytic activity after 48 hours compared to free enzyme. In first cycle of hydrolysis, immobilized enzyme hydrolyzed the cellulose and produced 19.5 ± 0.15 gm/L of glucose after 48 hours. On the contrary, free enzyme produced only 13.7 ± 0.25 gm/L of glucose in 48 hours. Immobilized enzyme maintained its stability and produced 6.15 ± 0.15 and 3.03 ± 0.25 gm/L of glucose in second and third cycle, respectively after 48 hours. Conclusion: This study will be very useful for sugar production because of enzyme binding efficiency and admirable reusability of immobilized enzyme, which leads to the significant increase in production of sugar from cellulosic materials.

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