Embling Production in Althaea officinalis L., Through Somatic Embryogenesis and Their Appraisal via Histological and Scanning Electron Microscopical Studies

2017 ◽  
Vol 182 (3) ◽  
pp. 1182-1197 ◽  
Author(s):  
Ruphi Naz ◽  
Mohammad Anis ◽  
Abdulrahman A. Alatar
Keyword(s):  
Somatic Embryogenesis ◽  
Althaea Officinalis ◽  
Electron Microscopical ◽  
Scanning Electron

Ultrastructure of the soil fungus, Geomyces pannorus

1984 ◽  
Vol 42 ◽  
pp. 738-739
Author(s):  
J. A. Traquair ◽  
E. G. Kokko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Low Temperatures ◽  
Soil Fungus ◽  
Organic Soils ◽  
Anatomical Studies ◽  
Transmission Electron ◽  
Electron Microscopical ◽  
Scanning Electron

With the advent of improved dehydration techniques, scanning electron microscopy has become routine in anatomical studies of fungi. Fine structure of hyphae and spore surfaces has been illustrated for many hyphomycetes, and yet, the ultrastructure of the ubiquitous soil fungus, Geomyces pannorus (Link) Sigler & Carmichael has been neglected. This presentation shows that scanning and transmission electron microscopical data must be correlated in resolving septal structure and conidial release in G. pannorus.Although it is reported to be cellulolytic but not keratinolytic, G. pannorus is found on human skin, animals, birds, mushrooms, dung, roots, and frozen meat in addition to various organic soils. In fact, it readily adapts to growth at low temperatures.

scholarly journals Induction of somatic embryogenesis in two cultivars of anthurium analysed by scanning electron microscopy

2019 ◽  
Vol 13 ◽  
pp. 1
Author(s):  
Priscila Bezerra Dos Santos Melo ◽  
Ana Cristina Portugal Pinto de Carvalho ◽  
Cândida Hermínia Campos de Magalhães Bertini ◽  
Celli Rodrigues Muniz ◽  
Adroaldo Guimarães Rossetti
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Somatic Embryogenesis ◽  
Callus Formation ◽  
Nodal Segments ◽  
Embryogenic Calli ◽  
Evaluation Period ◽  
Mean Values ◽  
Embryogenic Callus Formation ◽  
Scanning Electron

Somatic embryogenesis is an advantageous tool in the commercial production of micropropagated anthurium plantlets. As such, the aim of this study was to establish a protocol for the induction of somatic embryogenesis in Jureia and Luau cultivars. Defoliated nodal segments, 1.0 cm in length and containing one bud, were used as explants. The experimental design was completely randomised, in a 2 x 3 x 5 factorial scheme (cultivar: Jureia and Luau x auxin: 2,4-D, NAA and Picloram x concentration: 0, 2.5, 5.0, 7.5, and 10.0 μM), with 30 treatments in a scheme of plots split over time (15, 30, 45, 60, 75 and 90 days). The anatomy and percentage of embryogenic callus formation were analysed. The structures formed, analysed by scanning electron microscopy, corresponded to embryogenic calli. The Luau cultivar was superior in forming embryogenic calli. For the two cultivars, among the auxins under study, NAA demonstrated a greater induction potential for somatic embryogenesis, with the concentration of 7.5 μM giving the highest mean values. The 90-day evaluation period showed the maximum formation of embryogenic calli; however, mean values were fairly similar to the 75-day evaluation period. To induce embryogenic calli, therefore, it is suggested that the nodal segments be inoculated into a culture medium with added NAA growth regulator at a concentration of 7.5 μM, and that the explants remain in this medium for 75 days after inoculation.

scholarly journals Studies on the male and female copulatory apparatus of Trichuris globulosa (Nematoda, Trichuridae)

2007 ◽  
Vol 44 (4) ◽  
pp. 151-156 ◽  
Author(s):  
H. Kaur ◽  
C. Duggal
Keyword(s):  
Body Wall ◽  
Posterior Part ◽  
Male Body ◽  
Male And Female ◽  
Copulatory Apparatus ◽  
Electron Microscopical ◽  
Scanning Electron

AbstractThe process of copulation in Trichuris globulosa (see Linstow, 1901) Ransom, 1911 has been studied using morphological, histological and scanning electron microscopical techniques (Keilley et al., 1973). The ventral coiling of the posterior part of the male body around the female is achieved by specialized muscles of the ventral body-wall. The single simple pointed spicule emerges with the everting cirrus and acquires a J-shape. The spicule may help in keeping apart the cuticular brim of the non-spiny noneversible part of the vagina during insemination. The cloacal tube is long with a detached internal cuticle which is eversible to the outside as a cirrus. Eversion is brought about by the protrusion of the spicule and contraction of the muscles of the spicule pouch. During eversion part of the proximal cloacal tube is also extruded and forms the globular part of the cirrus. The globular part is covered with spines, which may help in retaining the male and female in copula. The vagina is differentiated into the proximal vagina uterine and distal vagina vera. The vagina vera has a distal eversible spiny part and a proximal noneversible unarmed part. In Trichuris globulosa, the vagina vera is shorter than the spicule.

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