Ultrastructure of the soil fungus, Geomyces pannorus

1984 ◽  
Vol 42 ◽  
pp. 738-739
Author(s):  
J. A. Traquair ◽  
E. G. Kokko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Low Temperatures ◽  
Soil Fungus ◽  
Organic Soils ◽  
Anatomical Studies ◽  
Transmission Electron ◽  
Electron Microscopical ◽  
Scanning Electron

With the advent of improved dehydration techniques, scanning electron microscopy has become routine in anatomical studies of fungi. Fine structure of hyphae and spore surfaces has been illustrated for many hyphomycetes, and yet, the ultrastructure of the ubiquitous soil fungus, Geomyces pannorus (Link) Sigler & Carmichael has been neglected. This presentation shows that scanning and transmission electron microscopical data must be correlated in resolving septal structure and conidial release in G. pannorus.Although it is reported to be cellulolytic but not keratinolytic, G. pannorus is found on human skin, animals, birds, mushrooms, dung, roots, and frozen meat in addition to various organic soils. In fact, it readily adapts to growth at low temperatures.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Microstructures of Oxidized Si3N4-8%Y2O3

1982 ◽  
Vol 40 ◽  
pp. 558-559
Author(s):  
K. Kuroda ◽  
H. C. Liu ◽  
A. H. Heuer ◽  
T. E. Mitchell
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Low Temperatures ◽  
Ambient Air ◽  
X Ray ◽  
Passive Oxidation ◽  
Transmission Electron ◽  
Doped Silicon ◽  
Apparent Stability ◽  
Scanning Electron

Introduction. There is considerable interest in the effect of oxidation on the microstructure of yttria-doped silicon nitride, since some compositions are unstable at low temperatures (=1000°C) despite their apparent stability at 1400°C (1). The material used in this study was the same commercial hotpressed Si3N4-8%Y2O3 (NCX-34) as one investigated previously, which had exhibited only passive oxidation during heating in air from 600° to 1400°C. Some thin (=100μm) specimens were oxidized at 800°, 1000° and 1450°C in ambient air and then ion-thinned from one side; others were ion-milled and oxidized. Specimens were examined by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) along with energy dispersive x-ray (EDX) analysis.

Fine structure of the microsymbiont of the actinorhizal root nodules of mountain mahogany (Cercocarpus ledifolius, family Rosaceae)

1989 ◽  
Vol 67 (1) ◽  
pp. 116-120 ◽  
Author(s):  
Susan M. Wood ◽  
William Newcomb ◽  
David Nelson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Root Nodules ◽  
Single Electron ◽  
Transmission Electron ◽  
Cercocarpus Ledifolius ◽  
Scanning Electron

Root nodules of Cercocarpus ledifolius Nutt. (mountain mahogany) were studied by light microscopy, scanning electron microscopy, and transmission electron microscopy to confirm the bacterial nature of the microsymbiont and to determine the morphology of the symbiotic vesicles. The microsymbiont is an actinomycete having two morphologies: septate hyphae (ca. 0.5 μm diam.) and ovoid- or elliptical-shaped nonseptate symbiotic vesicles (2.8 × 3.9 μm). Many of the symbiotic vesicles contain a single, electron-dense ovoid- or spherical-shaped structure, measuring 0.26 μm, whose function is unknown. The actinomycete is surrounded by a capsule that has electron-dense droplets in regions near hyphae. No spores or sporangia were observed in these nodules.

Fine structure and development of the zygospore of Rhizopus sexualis (Smith) Callen

1971 ◽  
Vol 263 (848) ◽  
pp. 71-100 ◽  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Probable Function ◽  
Cytoplasmic Organelles ◽  
Transmission Electron ◽  
Scanning Electron ◽  
Number Form

The development of the zygospore of Rhizopus sexualis (Smith) Callen was followed by light microscopy, transmission electron microscopy and scanning electron microscopy. Details of all stages, including dissolution of the fusion wall, delimitation of the gametangia by septa, and formation of the complex wall of the zygospore are described and illustrated. Changes in number, form and distribution of the organelles and the behaviour of the nuclei are described. The probable function of the cytoplasmic organelles and the possible mechanisms controlling development are discussed.

Cuticular Pores in a Marine Mite

1972 ◽  
Vol 30 ◽  
pp. 164-165
Author(s):  
John H. Crowe
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Epoxy Resin ◽  
Low Viscosity ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Scanning Electron

Several species of marine mites are known to possess elaborate cuticular pores, the function of which is not known (Newell, 1947). In the present investigation the fine structure of the cuticular pores in a Halacarid mite, Copidognathus curtusi was studied, with a view towards discovering the function of the pores.For scanning electron microscopy the animals were fixed in cacodylatebuffered glutaraldehyde, post-fixed in osmium, and freeze-dried. For transmission electron microscopy they were fixed as above, embedded in Spurr's low viscosity epoxy resin, and sectioned on diamond knives.C. curtusi was chosen for study because of the abundance of pores in its cuticle.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Scanning and Transmission Microscopy of Nematocyst Batteries in Epitheliomuscular Cells of Hydra

1971 ◽  
Vol 29 ◽  
pp. 410-411 ◽  
Author(s):  
Jane A. Westfall ◽  
S. Yamataka ◽  
Paul D. Enos
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Osmium Tetroxide ◽  
External Surface ◽  
Three Dimensional ◽  
Surface Structures ◽  
Transmission Microscopy ◽  
Transmission Electron ◽  
Freeze Dried ◽  
Scanning Electron

Scanning electron microscopy (SEM) provides three dimensional details of external surface structures and supplements ultrastructural information provided by transmission electron microscopy (TEM). Animals composed of watery jellylike tissues such as hydras and other coelenterates have not been considered suitable for SEM studies because of the difficulty in preserving such organisms in a normal state. This study demonstrates 1) the successful use of SEM on such tissue, and 2) the unique arrangement of batteries of nematocysts within large epitheliomuscular cells on tentacles of Hydra littoralis.Whole specimens of Hydra were prepared for SEM (Figs. 1 and 2) by the fix, freeze-dry, coat technique of Small and Màrszalek. The specimens were fixed in osmium tetroxide and mercuric chloride, freeze-dried in vacuo on a prechilled 1 Kg brass block, and coated with gold-palladium. Tissues for TEM (Figs. 3 and 4) were fixed in glutaraldehyde followed by osmium tetroxide. Scanning micrographs were taken on a Cambridge Stereoscan Mark II A microscope at 10 KV and transmission micrographs were taken on an RCA EMU 3G microscope (Fig. 3) or on a Hitachi HU 11B microscope (Fig. 4).

Fibroblasts During Hypertrophic Scar Formation and Resolution

1973 ◽  
Vol 31 ◽  
pp. 428-429
Author(s):  
C. W. Kischer
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Proliferation ◽  
Fine Structure ◽  
Metabolic Activity ◽  
Hypertrophic Scar ◽  
Normal Skin ◽  
Ground Substance ◽  
Hypertrophic Scars ◽  
Scanning Electron

The morphology of the fibroblasts changes markedly as the healing period from burn wounds progresses, through development of the hypertrophic scar, to resolution of the scar by a self-limiting process of maturation or therapeutic resolution. In addition, hypertrophic scars contain an increased cell proliferation largely made up of fibroblasts. This tremendous population of fibroblasts seems congruous with the abundance of collagen and ground substance. The fine structure of these cells should reflect some aspects of the metabolic activity necessary for production of the scar, and might presage the stage of maturation.A comparison of the fine structure of the fibroblasts from normal skin, different scar types, and granulation tissue has been made by transmission (TEM) and scanning electron microscopy (SEM).

Copper Localization in Cirrhotic Rat Liver by Scanning Electron Microscopy

1969 ◽  
Vol 27 ◽  
pp. 38-39 ◽  
Author(s):  
J. C. Russ ◽  
E. McNatt
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Electron Microscope ◽  
Copper Acetate ◽  
Lead Citrate ◽  
Dense Bodies ◽  
Transmission Electron ◽  
Electron Mode ◽  
Scanning Electron

In order to study the retention of copper in cirrhotic liver, rats were made cirrhotic by carbon tetrachloride inhalation twice weekly for three months and fed 0.2% copper acetate ad libidum in drinking water for one month. The liver tissue was fixed in osmium, sectioned approximately 2000 Å thick, and stained with lead citrate. The section was examined in a scanning electron microscope (JEOLCO JSM-2) in the transmission electron mode.Figure 1 shows a typical area that includes a red blood cell in a sinusoid, a disse, and a portion of the cytoplasm of a hepatocyte which contains several mitochondria, peribiliary dense bodies, glycogen granules, and endoplasmic reticulum.

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