Biological Characteristics and Effect of Human Umbilical Cord Mesenchymal Stem Cells (hUC-MSCs) Grafting with Blood Plasma on Bone Regeneration in Rats

2012 ◽  
Vol 63 (2) ◽  
pp. 171-181 ◽  
Author(s):  
Zhiguo Qu ◽  
Libin Guo ◽  
Guojun Fang ◽  
Zhenghong Cui ◽  
Shengnan Guo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Blood Plasma ◽  
Bone Regeneration ◽  
Umbilical Cord ◽  
Biological Characteristics ◽  
Human Umbilical Cord

scholarly journals AKT Pathway Affects Bone Regeneration in Nonunion Treated with Umbilical Cord-Derived Mesenchymal Stem Cells

2014 ◽  
Vol 71 (3) ◽  
pp. 1543-1551 ◽  
Author(s):  
Zhiguo Qu ◽  
Shengnan Guo ◽  
Guojun Fang ◽  
Zhenghong Cui ◽  
Ying Liu
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Blood Plasma ◽  
Umbilical Cord ◽  
Positive Control ◽  
Negative Control ◽  
Bone Morphogenetic Protein 2 ◽  
Human Umbilical Cord ◽  
Fracture Group ◽  
Rat Tibia

Abstract We have previously grafted human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) with blood plasma to treat rat tibia nonunion. To further examine the biological characteristics of this process, we applied an established hUC-MSCs-treated rat nonunion model with the addition of an inhibitor of AKT. SD rats (80) were randomly divided into four groups: a fracture group (positive control); a nonunion group (negative control); a hUC-MSCs grafting with blood plasma group; and a hUC-MSCs grafting with blood plasma & AKT blocker group. The animals were sacrificed under deep anesthesia at 4 and 8 weeks post fracture for analysis. The fracture line became less defined at 4 weeks and disappeared at 8 weeks postoperatively in both the hUC-MSCs grafting with blood plasma and grafting with blood plasma & the AKT blocker, which is similar to the fracture group. Histological immunofluorescence studies showed that the numbers of hUC-MSCs in the calluses were significantly higher in the hUC-MSCs grafting with blood plasma than those in group with the AKT blocker. More bone morphogenetic protein 2 and bone sialoprotein expression and less osteoprotegerin and bone gla protein expression were observed in the AKT blocker group compared to the hUC-MSCs grafting with blood plasma. AKT gene expression in the AKT blocker group was decreased 50 % compared to the hUC-MSCs with plasma group and decreased 70 % compared to the fracture group, while the elastic modulus was decreased. In summary, our work demonstrates that AKT may play a role in modulating osteogenesis induced by hUC-MSCs.

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