scholarly journals Moving Towards Induced Pluripotent Stem Cell-based Therapies with Artificial Intelligence and Machine Learning

2021 ◽  
Author(s):  
Claudia Coronnello ◽  
Maria Giovanna Francipane
Keyword(s):  
Artificial Intelligence ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Experimental Studies ◽  
Induced Pluripotent Stem Cell ◽  
Functional Differentiation ◽  
Cell Type ◽  
Induced Pluripotent ◽  
And Function ◽  
Intended Use

AbstractThe advent of induced pluripotent stem cell (iPSC) technology, which allows to transform one cell type into another, holds the promise to produce therapeutic cells and organs on demand. Realization of this objective is contingent on the ability to demonstrate quality and safety of the cellular product for its intended use. Bottlenecks and backlogs to the clinical use of iPSCs have been fully outlined and a need has emerged for safer and standardized protocols to trigger cell reprogramming and functional differentiation. Amidst great challenges, in particular associated with lengthy culture time and laborious cell characterization, a demand for faster and more accurate methods for the validation of cell identity and function at different stages of the iPSC manufacturing process has risen. Artificial intelligence-based methods are proving helpful for these complex tasks and might revolutionize the way iPSCs are managed to create surrogate cells and organs. Here, we briefly review recent progress in artificial intelligence approaches for evaluation of iPSCs and their derivatives in experimental studies. Graphical Abstract

Abstract 379: Effects of Microgravity on Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Structure and Function

2018 ◽  
Vol 123 (Suppl_1) ◽  
Author(s):  
Alexa Wnorowski ◽  
Arun Sharma ◽  
Haodong Chen ◽  
Haodi Wu ◽  
Ning-Yi Shao ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Structure And Function ◽  
Induced Pluripotent ◽  
And Function

scholarly journals Induced-Pluripotent-Stem-Cell-Derived Primitive Macrophages Provide a Platform for Modeling Tissue-Resident Macrophage Differentiation and Function

Immunity ◽  
2020 ◽  
Vol 52 (2) ◽  
pp. 417-418
Author(s):  
Kazuyuki Takata ◽  
Tatsuya Kozaki ◽  
Christopher Zhe Wei Lee ◽  
Morgane Sonia Thion ◽  
Masayuki Otsuka ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Macrophage Differentiation ◽  
Resident Macrophage ◽  
Induced Pluripotent ◽  
And Function

scholarly journals Effects of Spaceflight on Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Structure and Function

2019 ◽  
Vol 13 (6) ◽  
pp. 960-969 ◽  
Author(s):  
Alexa Wnorowski ◽  
Arun Sharma ◽  
Haodong Chen ◽  
Haodi Wu ◽  
Ning-Yi Shao ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Structure And Function ◽  
Induced Pluripotent ◽  
And Function

scholarly journals Donor‐Dependent and Other Nondefined Factors have Greater Influence on the Hepatic Phenotype than the Starting Cell Type in Induced Pluripotent Stem Cell Derived Hepatocyte‐Like Cells

2017 ◽  
Vol 6 (8) ◽  
pp. 1751-1751 ◽  
Author(s):  
James A. Heslop ◽  
Richard Kia ◽  
Christopher S. Pridgeon ◽  
Rowena L. Sison‐Young ◽  
Triantafillos Liloglou ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Cell Type ◽  
Induced Pluripotent

scholarly journals Induced-Pluripotent-Stem-Cell-Derived Primitive Macrophages Provide a Platform for Modeling Tissue-Resident Macrophage Differentiation and Function

Immunity ◽  
2017 ◽  
Vol 47 (1) ◽  
pp. 183-198.e6 ◽  
Author(s):  
Kazuyuki Takata ◽  
Tatsuya Kozaki ◽  
Christopher Zhe Wei Lee ◽  
Morgane Sonia Thion ◽  
Masayuki Otsuka ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Macrophage Differentiation ◽  
Resident Macrophage ◽  
Induced Pluripotent ◽  
And Function

scholarly journals Donor-Dependent and Other Nondefined Factors Have Greater Influence on the Hepatic Phenotype Than the Starting Cell Type in Induced Pluripotent Stem Cell Derived Hepatocyte-Like Cells

2017 ◽  
Vol 6 (5) ◽  
pp. 1321-1331 ◽  
Author(s):  
James A. Heslop ◽  
Richard Kia ◽  
Christopher S. Pridgeon ◽  
Rowena L. Sison-Young ◽  
Triantafillos Liloglou ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Cell Type ◽  
Induced Pluripotent

scholarly journals LRRK2 is recruited to phagosomes and co-recruits Rab8 and Rab10 in human pluripotent stem cell-derived macrophages

2019 ◽  
Author(s):  
Heyne Lee ◽  
Rowan Flynn ◽  
Ishta Sharma ◽  
Phillippa J. Carling ◽  
Francesca J. Nicholls ◽  
...  
Keyword(s):  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Induced Pluripotent Stem Cell ◽  
Interferon Γ ◽  
Phagosome Maturation ◽  
Immune Disorders ◽  
Lrrk2 Kinase ◽  
Induced Pluripotent ◽  
And Function ◽  
Phagocytic Uptake

SummaryThe Parkinson’s disease-associated gene, LRRK2, is also associated with immune disorders and infectious disease, and is expressed in immune subsets. Here, we characterise a platform for interrogating the expression and function of endogenous LRRK2 in authentic human phagocytes, using human induced Pluripotent Stem Cell-derived macrophages and microglia. Endogenous LRRK2 is expressed and upregulated by interferon-γ in these cells, including a 187kD cleavage product. Using LRRK2 knockout and G2019S isogenic repair lines, we find that LRRK2 is not involved in initial phagocytic uptake of bioparticles, but is recruited to LAMP1(+)/Rab9(+) ‘maturing’ phagosomes, and LRRK2 kinase inhibition enhances its residency at the phagosome. Importantly, LRRK2 is required for Rab8a and Rab10 recruitment to phagosomes, implying that LRRK2 operates at the intersection between phagosome maturation and recycling pathways in these professional phagocytes.

Sign in / Sign up

Export Citation Format

Share Document