mRNA Quantification After Fluorescence Activated Cell Sorting Using Locked Nucleic Acid Probes

2011 ◽  
Vol 49 (1) ◽  
pp. 42-47 ◽  
Author(s):  
Rie Maruo ◽  
Hiroya Yamada ◽  
Mikio Watanabe ◽  
Yoh Hidaka ◽  
Yoshinori Iwatani ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Cell Sorting ◽  
Locked Nucleic Acid ◽  
Fluorescence Activated Cell Sorting ◽  
Nucleic Acid Probes ◽  
Mrna Quantification

MicroRNA detection by northern blotting using locked nucleic acid probes

2008 ◽  
Vol 3 (2) ◽  
pp. 190-196 ◽  
Author(s):  
Éva Várallyay ◽  
József Burgyán ◽  
Zoltán Havelda
Keyword(s):  
Nucleic Acid ◽  
Northern Blotting ◽  
Locked Nucleic Acid ◽  
Nucleic Acid Probes ◽  
Microrna Detection

Ultrasensitive SERS detection of highly homologous miRNAs by generating 3D organic-nanoclusters and a functionalized chip with locked nucleic acid probes

2018 ◽  
Vol 54 (95) ◽  
pp. 13431-13434 ◽  
Author(s):  
Mengting Zhu ◽  
Zhaomei Sun ◽  
Zhen Zhang ◽  
Shusheng Zhang
Keyword(s):  
Nucleic Acid ◽  
Locked Nucleic Acid ◽  
Nucleotide Difference ◽  
Single Nucleotide ◽  
Nucleic Acid Probes ◽  
Sers Detection ◽  
New Finding

Herein, a new finding is reported that 3D organic-nanoclusters (3DONs) with superior SERS properties as an original reporter could accurately and sensitively distinguish microRNAs (miRNAs) with highly similar sequences, even with a single-nucleotide difference.

scholarly journals Detection of mRNA in living cells by double-stranded locked nucleic acid probes

The Analyst ◽  
2013 ◽  
Vol 138 (17) ◽  
pp. 4777 ◽  
Author(s):  
Reza Riahi ◽  
Zachary Dean ◽  
Ting-Hsiang Wu ◽  
Michael A. Teitell ◽  
Pei-Yu Chiou ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Living Cells ◽  
Locked Nucleic Acid ◽  
Nucleic Acid Probes

scholarly journals Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses

2015 ◽  
Vol 53 (8) ◽  
pp. 2722-2726 ◽  
Author(s):  
Jasper Fuk-Woo Chan ◽  
Garnet Kwan-Yue Choi ◽  
Alan Ka-Lun Tsang ◽  
Kah-Meng Tee ◽  
Ho-Yin Lam ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Reverse Transcription ◽  
Locked Nucleic Acid ◽  
Small Rna Sequencing ◽  
Nucleic Acid Probes ◽  
Reverse Transcription Pcr ◽  
Highly Sensitive ◽  
Pcr Assays ◽  
Human Coronaviruses

Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) and other human coronaviruses. Analytical and clinical evaluations showed their noninferiority to a commercial multiplex PCR test for the detection of these coronaviruses.

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