Ultrasensitive SERS detection of highly homologous miRNAs by generating 3D organic-nanoclusters and a functionalized chip with locked nucleic acid probes

2018 ◽  
Vol 54 (95) ◽  
pp. 13431-13434 ◽  
Author(s):  
Mengting Zhu ◽  
Zhaomei Sun ◽  
Zhen Zhang ◽  
Shusheng Zhang
Keyword(s):  
Nucleic Acid ◽  
Locked Nucleic Acid ◽  
Nucleotide Difference ◽  
Single Nucleotide ◽  
Nucleic Acid Probes ◽  
Sers Detection ◽  
New Finding

Herein, a new finding is reported that 3D organic-nanoclusters (3DONs) with superior SERS properties as an original reporter could accurately and sensitively distinguish microRNAs (miRNAs) with highly similar sequences, even with a single-nucleotide difference.

Nanopore-Based Selective Discrimination of MicroRNAs with Single-Nucleotide Difference Using Locked Nucleic Acid-Modified Probes

2016 ◽  
Vol 88 (21) ◽  
pp. 10540-10546 ◽  
Author(s):  
Dongmei Xi ◽  
Jizhen Shang ◽  
Enguo Fan ◽  
Jinmao You ◽  
Shusheng Zhang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Locked Nucleic Acid ◽  
Nucleotide Difference ◽  
Single Nucleotide

scholarly journals Locked Nucleic Acid Thymine Monomer Probe Identifies Four Single-Nucleotide Variants by Melting Temperature

2017 ◽  
Vol 112 (3) ◽  
pp. 331a-332a
Author(s):  
Judy M. Obliosca ◽  
Sara Y. Cheng ◽  
Yu-An Chen ◽  
Mariana F. Llanos ◽  
Yen-Liang Liu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Melting Temperature ◽  
Locked Nucleic Acid ◽  
Single Nucleotide Variants ◽  
Single Nucleotide

MicroRNA detection by northern blotting using locked nucleic acid probes

2008 ◽  
Vol 3 (2) ◽  
pp. 190-196 ◽  
Author(s):  
Éva Várallyay ◽  
József Burgyán ◽  
Zoltán Havelda
Keyword(s):  
Nucleic Acid ◽  
Northern Blotting ◽  
Locked Nucleic Acid ◽  
Nucleic Acid Probes ◽  
Microrna Detection

mRNA Quantification After Fluorescence Activated Cell Sorting Using Locked Nucleic Acid Probes

2011 ◽  
Vol 49 (1) ◽  
pp. 42-47 ◽  
Author(s):  
Rie Maruo ◽  
Hiroya Yamada ◽  
Mikio Watanabe ◽  
Yoh Hidaka ◽  
Yoshinori Iwatani ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Cell Sorting ◽  
Locked Nucleic Acid ◽  
Fluorescence Activated Cell Sorting ◽  
Nucleic Acid Probes ◽  
Mrna Quantification

Application of a lanthanide fluorescent chelate label for detection of single-nucleotide mutations with peptide nucleic acid probes

2006 ◽  
Vol 355 (2) ◽  
pp. 278-284 ◽  
Author(s):  
Kimikazu Hashino ◽  
Masahiro Ito ◽  
Keisuke Ikawa ◽  
Chihiro Hosoya ◽  
Takuya Nishioka ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Peptide Nucleic Acid ◽  
Single Nucleotide ◽  
Nucleic Acid Probes

scholarly journals A Locked Nucleic Acid Probe Based on Selective Salt-Induced Effect Detects Single Nucleotide Polymorphisms

2015 ◽  
Vol 2015 ◽  
pp. 1-6
Author(s):  
Jing Zhang ◽  
Huizhe Wu ◽  
Qiuchen Chen ◽  
Pengfei Zhao ◽  
Haishan Zhao ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Single Nucleotide Polymorphisms ◽  
Room Temperature ◽  
Genetic Mutation ◽  
Locked Nucleic Acid ◽  
Nucleotide Polymorphisms ◽  
Buffer Solution ◽  
Single Nucleotide ◽  
Real Time Quantitative Pcr ◽  
Pcr Products

Detection of single based genetic mutation by using oligonucleotide probes is one of the common methods of detecting single nucleotide polymorphisms at known loci. In this paper, we demonstrated a hybridization system which included a buffer solution that produced selective salt-induced effect and a locked nucleic acid modified 12 nt oligonucleotide probe. The hybridization system is suitable for hybridization under room temperature. By using magnetic nanoparticles as carriers for PCR products, the SNPs (MDR1C3435T/A) from 45 volunteers were analyzed, and the results were consistent with the results from pyrophosphoric acid sequencing. The method presented in this paper differs from the traditional method of using molecular beacons to detect SNPs in that it is suitable for research institutions lacking real-time quantitative PCR detecting systems, to detect PCR products at room temperature.

Locked nucleic acid (LNA) induced effect on the hybridization and fluorescence properties of oligodeoxyribonucleotides modified with nucleobase-functionalized DNA monomers

2015 ◽  
Vol 13 (26) ◽  
pp. 7236-7247 ◽  
Author(s):  
Mamta Kaura ◽  
Patrick J. Hrdlicka
Keyword(s):  
Nucleic Acid ◽  
Single Nucleotide Polymorphisms ◽  
Locked Nucleic Acid ◽  
Fluorescence Properties ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

Mixmer oligonucleotides modified with LNA and C5-pyrene-functionalized DNA monomers are shown to display interesting fluorescence properties for the discrimination of single nucleotide polymorphisms (SNPs).

scholarly journals Detection of mRNA in living cells by double-stranded locked nucleic acid probes

The Analyst ◽  
2013 ◽  
Vol 138 (17) ◽  
pp. 4777 ◽  
Author(s):  
Reza Riahi ◽  
Zachary Dean ◽  
Ting-Hsiang Wu ◽  
Michael A. Teitell ◽  
Pei-Yu Chiou ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Living Cells ◽  
Locked Nucleic Acid ◽  
Nucleic Acid Probes
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