Protein Production in Yarrowia lipolytica Via Fusion to the Secreted Lipase Lip2p

2013 ◽  
Vol 56 (1) ◽  
pp. 79-90 ◽  
Author(s):  
Thomas Hofmeyer ◽  
Siyavuya Ishmael Bulani ◽  
Julius Grzeschik ◽  
Simon Krah ◽  
Bernhard Glotzbach ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Protein Production ◽  
Secreted Lipase

Impact of oxygen availability on heterologous geneexpression and polypeptide secretion dynamics in Yarrowia lipolytica ‐based protein production platforms

Yeast ◽  
2020 ◽  
Vol 37 (9-10) ◽  
pp. 559-568 ◽  
Author(s):  
Maria Gorczyca ◽  
Jan Kaźmierczak ◽  
Sebastien Steels ◽  
Patrick Fickers ◽  
Ewelina Celińska
Keyword(s):  
Yarrowia Lipolytica ◽  
Protein Production ◽  
Oxygen Availability

scholarly journals A novel and effective Cre/lox-based genetic tool for repeated, targeted and markerless integration in Yarrowia lipolytica

2020 ◽  
Author(s):  
Qinghua Zhou ◽  
Liangcheng Jiao ◽  
Wenjuan Li ◽  
Zhiming Hu ◽  
Yunchong Li ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Protein Production ◽  
Genome Engineering ◽  
Selection Marker ◽  
The Novel ◽  
Genetic Tool ◽  
Recombination System ◽  
Marker Free ◽  
Free Gene ◽  
Gene Integration

AbstractThe unconventional yeast Yarrowia lipolytica is extensively applied in bioproduction fields owing to its excellent metabolite and protein production ability. Nonetheless, utilization of this promising host is still restricted by limited availability of precise and effective gene integration tools. In this study, a novel and efficient genetic tool was developed for targeted, repeated, and markerless gene integration based on Cre/lox sitespecific recombination system. The developed tool required only a single selection marker and could completely excise all of the unnecessary sequences. A total of three plasmids were created and seven rounds of marker-free gene integration were examined in Y. lipolytica. All the integration efficiencies remained above 90%, and analysis of protein production and growth characteristics of the engineered strains confirmed that genome modification via the novel genetic tool was feasible. Further work also confirmed the genetic tool was effective for integration of other genes, loci, and strains. Thus, this study significantly promotes the application of Cre/lox system and presents a powerful tool for genome engineering in Y. lipolytica.

Biochemical characterization of Yarrowia lipolytica LIP8, a secreted lipase with a cleavable C-terminal region

2015 ◽  
Vol 1851 (2) ◽  
pp. 129-140 ◽  
Author(s):  
Jannet Kamoun ◽  
Mathieu Schué ◽  
Wala Messaoud ◽  
Justine Baignol ◽  
Vanessa Point ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Biochemical Characterization ◽  
Terminal Region ◽  
Secreted Lipase

scholarly journals A Novel Cre/lox-Based Genetic Tool for Repeated, Targeted and Markerless Gene Integration in Yarrowia lipolytica

2021 ◽  
Vol 22 (19) ◽  
pp. 10739
Author(s):  
Qinghua Zhou ◽  
Liangcheng Jiao ◽  
Wenjuan Li ◽  
Zhiming Hu ◽  
Yunchong Li ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Protein Production ◽  
Genome Engineering ◽  
Selection Marker ◽  
The Novel ◽  
Genetic Tool ◽  
Recombination System ◽  
Marker Free ◽  
Free Gene ◽  
Gene Integration

The unconventional yeast Yarrowia lipolytica is extensively applied in bioproduction fields owing to its excellent metabolite and protein production ability. Nonetheless, utilization of this promising host is still restricted by the limited availability of precise and effective gene integration tools. In this study, a novel and efficient genetic tool was developed for targeted, repeated, and markerless gene integration based on Cre/lox site-specific recombination system. The developed tool required only a single selection marker and could completely excise the unnecessary sequences. A total of three plasmids were created and seven rounds of marker-free gene integration were examined in Y. lipolytica. All the integration efficiencies remained above 90%, and analysis of the protein production and growth characteristics of the engineered strains confirmed that genome modification via the novel genetic tool was feasible. Further work also confirmed that the genetic tool was effective for the integration of other genes, loci, and strains. Thus, this study significantly promotes the application of the Cre/lox system and presents a powerful tool for genome engineering in Y. lipolytica.

scholarly journals Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica

2017 ◽  
Vol 16 (1) ◽  
Author(s):  
Rémi Dulermo ◽  
François Brunel ◽  
Thierry Dulermo ◽  
Rodrigo Ledesma-Amaro ◽  
Jérémy Vion ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Protein Production ◽  
Variable Strength

Use of ars18 based vectors to increase protein production in Yarrowia lipolytica

1991 ◽  
Vol 19 (2-3) ◽  
pp. 259-270 ◽  
Author(s):  
J.M. Nicaud ◽  
P. Fournier ◽  
C. La Bonnardière ◽  
M. Chasles ◽  
C. Gaillardin
Keyword(s):  
Yarrowia Lipolytica ◽  
Protein Production

Feeding strategy impacts heterologous protein production in Yarrowia lipolytica fed‐batch cultures—Insight into the role of osmolarity

Yeast ◽  
2019 ◽  
Vol 36 (5) ◽  
pp. 305-318 ◽  
Author(s):  
Monika Kubiak ◽  
Monika Borkowska ◽  
Wojciech Białas ◽  
Paulina Korpys ◽  
Ewelina Celińska
Keyword(s):  
Yarrowia Lipolytica ◽  
Protein Production ◽  
Heterologous Protein ◽  
Feeding Strategy ◽  
Heterologous Protein Production ◽  
Fed Batch ◽  
Batch Cultures ◽  
Insight Into

scholarly journals Waste Cooking Oil as Substrate for Single Cell Protein Production by Yeast Yarrowia lipolytica

2020 ◽  
Vol 24 (3) ◽  
pp. 457-469
Author(s):  
Kriss Spalvins ◽  
Zane Geiba ◽  
Zane Kusnere ◽  
Dagnija Blumberga
Keyword(s):  
Carbon Source ◽  
Single Cell ◽  
Yarrowia Lipolytica ◽  
Protein Production ◽  
Waste Cooking Oil ◽  
Single Cell Protein ◽  
Cell Protein ◽  
Cooking Oil ◽  
Cooking Oils ◽  
Single Cell Protein Production

AbstractCooking oils are widely used in food preparation. During cooking, harmful compounds are formed in oils, therefore utilization of used cooking oils (waste cooking oils) is limited. Single cell protein (SCP) is dietary protein, which can be produced from various protein-rich microorganisms that are capable of utilizing industrial by-products such as waste cooking oil (WCO). In this study the utilization of industrial WCO (obtained from local potato chips manufacturer) as a carbon source for single cell protein production by yeast Yarrowia lipolytica was assessed. The medium containing 27.5 g/L WCO and C/N ratio of 5–10 for batch fermentations was determined to be the optimal composition for SCP production. In this study, the highest reported Yarrowia lipolytica biomass concentration (57.37 g/L) was achieved when WCO was used as the main carbon source. Protein concentrations were relatively low (12.6 %), which also affected the final protein yield (7.23 g/L). The resulting biomass accumulated low concentrations of toxic malondialdehyde (MDA) (2.32 mg MDA/kg) compared to concentrations initially detected in the WCO itself (30.87 mg MDA/kg). To the best of the authors knowledge this is the first study to report on MDA decrease via microbial fermentations.

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