Validation and cost-effectiveness of an alternative method to quantify Batrachochytrium dendrobatidis infection in amphibian samples using real-time PCR

2017 ◽  
Vol 28 (4) ◽  
pp. 687-692 ◽  
Author(s):  
Stefano Canessa ◽  
Marco Maggesi ◽  
Sebastiano Salvidio ◽  
Elena Grasselli
Keyword(s):  
Cost Effectiveness ◽  
Real Time ◽  
Real Time Pcr ◽  
Batrachochytrium Dendrobatidis ◽  
Alternative Method

scholarly journals First detection of the amphibian chytrid fungus (Batrachochytridium dendrobatidis) in free- living anuran populations in Greece

2018 ◽  
Vol 67 (4) ◽  
pp. 253
Author(s):  
P. N. AZMANIS ◽  
I. STRACHINIS ◽  
P. LYMBERAKIS ◽  
R. E. MARSCHANG
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Batrachochytrium Dendrobatidis ◽  
Chytrid Fungus ◽  
Free Living ◽  
Bufo Viridis ◽  
First Report ◽  
Water Frogs ◽  
Preliminary Study ◽  
Amphibian Chytrid Fungus

The amphibian chytrid fungus (Batrachochytrium dendrobatidis) is a widespread, cosmopolitan pathogen largely affecting free-living amphibian populations. So far there are no published studies for the presence of B. dendrobatidis in Greece. In this preliminary study we sampled 59 metamorphosed anurans from four Greek wetlands. Five samples were positive for the fungus by real-time PCR. B. dendrobatidis was detected in three species (Bufo viridis, Pelophylax epeiroticus, Pelophylax ridibundus) but not in endangered endemic Karpathos water frogs (Pelophylax cerigensis). This is the first report of the amphibian chytrid fungus (Batrachochytrium dendrobatidis) in free-living anuran populations from Greece.

scholarly journals Duplex Real-Time PCR for Rapid Simultaneous Detection of Batrachochytrium dendrobatidis and Batrachochytrium salamandrivorans in Amphibian Samples

2013 ◽  
Vol 51 (12) ◽  
pp. 4173-4177 ◽  
Author(s):  
M. Blooi ◽  
F. Pasmans ◽  
J. E. Longcore ◽  
A. Spitzen-van der Sluijs ◽  
F. Vercammen ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Batrachochytrium Dendrobatidis ◽  
Simultaneous Detection ◽  
Batrachochytrium Salamandrivorans

scholarly journals Capillary Electrophoresis of PCR fragments with 5’-labelled primers for testing the SARS-Cov-2

2020 ◽  
Author(s):  
Juan Gómez ◽  
Santiago Melón ◽  
José A. Boga ◽  
Marta E. Alvarez-Argüelles ◽  
Susana Rojo-Alba ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Real Time ◽  
Study Design ◽  
Real Time Pcr ◽  
Viral Genome ◽  
Alternative Method ◽  
Fluorescent Pcr

AbstractBackgroundDue to the huge demand for SARS-Cov-2 determination, alternatives to the standard qtPCR tests are potentially useful for increasing the number of samples screened. Our aim was to develop a direct fluorescent PCR capillary-electrophoresis detection of the viral genome. We validated this approach on several SARS-Cov-2 positive and negative samples.Study designWe isolated the naso-pharingeal RNA from 20 positive and 10 negative samples. The cDNA was synthesised and two fragments of the SARS-Cov-2 were amplified. One of the primers for each pair was 5’-end fluorochrome labelled. The amplifications were subjected to capillary electrophoresis in ABI3130 sequencers to visualize the fluorescent peaks.ResultsThe two SARS-Cov-2 fragments were successfully amplified in the positive samples, while the negative samples did not render fluorescent peaks.ConclusionWe describe and alternative method to identify the SARS-Cov-2 genome that could be scaled to the analysis of approximately 100 samples in less than 5 hours. By combining a standard PCR with capillary electrophoresis our approach would overcome the limits imposed to many labs by the qtPCR (lack of reactive and real-time PCR equipment) and increase the testing capacity.

Combining ethidium monoazide treatment with real-time PCR selectively quantifies viable Batrachochytrium dendrobatidis cells

2013 ◽  
Vol 117 (2) ◽  
pp. 156-162 ◽  
Author(s):  
Mark Blooi ◽  
An Martel ◽  
Francis Vercammen ◽  
Frank Pasmans
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Batrachochytrium Dendrobatidis ◽  
Ethidium Monoazide

scholarly journals BATRACHOCHYTRIUM DENDROBATIDIS OCCURRENCE IN DEAD AMPHIBIANS OF CENTRAL MEXICO: A REPORT OF Ambystoma altamirani AND Lithobates montezumae.

2021 ◽  
Vol 4 (1) ◽  
pp. 173
Author(s):  
Gabriela Parra Olea
Keyword(s):  
Infectious Disease ◽  
Real Time ◽  
Real Time Pcr ◽  
Batrachochytrium Dendrobatidis ◽  
Central Mexico

Chytridiomycosis, caused by the fungus Batrachochytrium dendrobatidis (Bd) and B. salamandrivorans (Bsal), is an infectious disease of amphibians linked to mass amphibian die-offs worldwide. In this study, we sampled four dead and dying individuals of Ambystoma altamirani and Lithobates montezumae to detect the presence of Bd and Bsal. By real-time PCR (qPCR) and histopathology methods, we found the presence of Bd and the absence of Bsal in all individuals sampled. Our study indicates that chytridiomycosis may act as a threat for these species and highlight that future surveys are urgently needed to evaluate the Bd infection on populations of A. altamirani and L. montezumae.

scholarly journals An Observational Propensity Score-Matched Study to Evaluate Cost-Effectiveness of a Real-Time Pcr-Based Assay in Patients with Suspected Sepsisk

2014 ◽  
Vol 17 (7) ◽  
pp. A672-A673 ◽  
Author(s):  
O. Zaniolo ◽  
M. Povero ◽  
L. Pradelli ◽  
B. Pizzorno ◽  
N. Mancini
Keyword(s):  
Cost Effectiveness ◽  
Propensity Score ◽  
Real Time ◽  
Real Time Pcr ◽  
Matched Study
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