scholarly journals Fluorescence Lifetime Imaging Combined with Conventional Intravascular Ultrasound for Enhanced Assessment of Atherosclerotic Plaques: an Ex Vivo Study in Human Coronary Arteries

2015 ◽  
Vol 8 (4) ◽  
pp. 253-263 ◽  
Author(s):  
Hussain Fatakdawala ◽  
Dimitris Gorpas ◽  
John W. Bishop ◽  
Julien Bec ◽  
Dinglong Ma ◽  
...  
Keyword(s):  
Intravascular Ultrasound ◽  
Coronary Arteries ◽  
Fluorescence Lifetime ◽  
Ex Vivo ◽  
Atherosclerotic Plaques ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Ex Vivo Study

scholarly journals Fluorescence Lifetime Imaging and Intravascular Ultrasound: Co-Registration Study Using Ex Vivo Human Coronaries

2015 ◽  
Vol 34 (1) ◽  
pp. 156-166 ◽  
Author(s):  
Dimitris Gorpas ◽  
Hussain Fatakdawala ◽  
Julien Bec ◽  
Dinglong Ma ◽  
Diego R. Yankelevich ◽  
...  
Keyword(s):  
Intravascular Ultrasound ◽  
Fluorescence Lifetime ◽  
Ex Vivo ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

scholarly journals Distinct metabolic profiles in Drosophila sperm and somatic tissues revealed by two-photon NAD(P)H and FAD autofluorescence lifetime imaging

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Cornelia Wetzker ◽  
Klaus Reinhardt
Keyword(s):  
Fluorescence Lifetime ◽  
Biological Diversity ◽  
Ex Vivo ◽  
Substantial Reduction ◽  
Metabolic Profiles ◽  
Fluorescence Lifetime Imaging ◽  
Two Photon ◽  
Lifetime Imaging ◽  
Somatic Tissues ◽  
Two Photon Fluorescence

AbstractMetabolic profiles vary across all levels of biological diversity, from cells to taxa. Two-photon fluorescence lifetime imaging microscopy (FLIM) facilitates metabolic characterisation of biological specimens by assaying the intrinsic autofluorescence of the ubiquitous coenzymes NAD(P)H and FAD. The potential of this method for characterising the diversity of organismal metabolism remains largely untapped. Using FLIM in Drosophila melanogaster, we show tissue-specificity in fluorescence lifetime that reflects variation in redox patterns. In particular, sperm cells exhibited elevated glycolysis relative to other tissues. We also show that sperm metabolism is phenotypically plastic: compared to male-stored sperm, sperm stored in the female’s storage organ showed a substantial reduction in the protein-bound FAD lifetime fraction but no change in the NAD(P)H profile. This study represents the first ex vivo investigation of sperm metabolism using FLIM.

scholarly journals Fluorescence lifetime imaging for the characterization of the biochemical composition of atherosclerotic plaques

2011 ◽  
Vol 16 (9) ◽  
pp. 096018 ◽  
Author(s):  
Jennifer Phipps ◽  
Yinghua Sun ◽  
Ramez Saroufeem ◽  
Nisa Hatami ◽  
Michael C. Fishbein ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Biochemical Composition ◽  
Atherosclerotic Plaques ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

scholarly journals In Vivo Time Domain Optical Imaging of Renal Ischemia-Reperfusion Injury: Discrimination Based on Fluorescence Lifetime

2007 ◽  
Vol 6 (5) ◽  
pp. 7290.2007.00030 ◽  
Author(s):  
Abedelnasser Abulrob ◽  
Eric Brunette ◽  
Jacqueline Slinn ◽  
Ewa Baumann ◽  
Danica Stanimirovic
Keyword(s):  
Optical Imaging ◽  
Fluorescence Lifetime ◽  
Fluorescence Intensity ◽  
Time Domain ◽  
Ex Vivo ◽  
Renal Ischemia ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Lifetime Analysis

Fluorescence lifetime is an intrinsic parameter of the fluorescent probe, independent of the probe concentration but sensitive to changes in the surrounding microenvironment. Therefore, fluorescence lifetime imaging could potentially be applied to in vivo diagnostic assessment of changes in the tissue microenvironment caused by disease, such as ischemia. The aim of this study was to evaluate the utility of noninvasive fluorescence lifetime imaging in distinguishing between normal and ischemic kidney tissue in vivo. Mice were subjected to 60-minute unilateral kidney ischemia followed by 6-hour reperfusion. Animals were then injected with the near-infrared fluorescence probe Cy5.5 or saline and imaged using a time-domain small-animal optical imaging system. Both fluorescence intensity and lifetime were acquired. The fluorescence intensity of Cy5.5 was clearly reduced in the ischemic compared with the contralateral kidney, and the fluorescence lifetime of Cy5.5 was not detected in the ischemic kidney, suggesting reduced kidney clearance. Interestingly, the two-component lifetime analysis of endogenous fluorescence at 700 nm distinguished renal ischemia in vivo without the need for Cy5.5 injection for contrast enhancement. The average fluorescence lifetime of endogenous tissue fluorophores was a sensitive indicator of kidney ischemia ex vivo. The study suggests that fluorescence lifetime analysis of endogenous tissue fluorophores could be used to discriminate ischemic or necrotic tissues by noninvasive in vivo or ex vivo organ imaging.

Sign in / Sign up

Export Citation Format

Share Document