scholarly journals Rotlicht-gesteuerter viraler Gentransfer mit Einzelzellauflösung

BIOspektrum ◽  
2021 ◽  
Vol 27 (6) ◽  
pp. 620-622
Author(s):  
Maximilian Hörner ◽  
Wilfried Weber
Keyword(s):  
Gene Transfer ◽  
Cell Lines ◽  
Single Cells ◽  
Primary Cells ◽  
Spatially Resolved ◽  
Efficient Gene ◽  
Native Cell ◽  
Local Illumination

AbstractAvailable methods for efficient gene transfer into user-selected or even single cells suffer from high invasiveness or the need for complicated equipment. Here, we present a technology for the light-guided transduction of native cell lines and primary cells by adeno-associated viral (AAV) vectors. We demonstrate the spatially resolved transduction of different cells with different genes within one culture and the selective transduction of single cells by local illumination.

scholarly journals Novel retroviral packaging cell lines: complementary tropisms and improved vector production for efficient gene transfer

Gene Therapy ◽  
1997 ◽  
Vol 4 (6) ◽  
pp. 600-610 ◽  
Author(s):  
SP Forestell ◽  
JS Dando ◽  
J Chen ◽  
P de Vries ◽  
E Böhnlein ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Cell Lines ◽  
Packaging Cell ◽  
Efficient Gene

A PEG-Based Biocompatible Block Catiomer with High Buffering Capacity for the Construction of Polyplex Micelles Showing Efficient Gene Transfer toward Primary Cells

ChemMedChem ◽  
2006 ◽  
Vol 1 (4) ◽  
pp. 439-444 ◽  
Author(s):  
Naoki Kanayama ◽  
Shigeto Fukushima ◽  
Nobuhiro Nishiyama ◽  
Keiji Itaka ◽  
Woo-Dong Jang ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Buffering Capacity ◽  
Primary Cells ◽  
Efficient Gene

scholarly journals Spatiotemporally confined red light-controlled gene delivery at single-cell resolution using adeno-associated viral vectors

2021 ◽  
Vol 7 (25) ◽  
pp. eabf0797
Author(s):  
Maximilian Hörner ◽  
Carolina Jerez-Longres ◽  
Anna Hudek ◽  
Sebastian Hook ◽  
O. Sascha Yousefi ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Single Cell ◽  
Genetic Information ◽  
Viral Vectors ◽  
Single Cells ◽  
Red Light ◽  
Genetic Research ◽  
Vector System ◽  
Target Cells ◽  
Spatially Resolved

Methodologies for the controlled delivery of genetic information into target cells are of utmost importance for genetic engineering in both fundamental and applied research. However, available methods for efficient gene transfer into user-selected or even single cells suffer from low throughput, the need for complicated equipment, high invasiveness, or side effects by off-target viral uptake. Here, we engineer an adeno-associated viral (AAV) vector system that transfers genetic information into native target cells upon illumination with cell-compatible red light. This OptoAAV system allows adjustable and spatially resolved gene transfer down to single-cell resolution and is compatible with different cell lines and primary cells. Moreover, the sequential application of multiple OptoAAVs enables spatially resolved transduction with different transgenes. The approach presented is likely extendable to other classes of viral vectors and is expected to foster advances in basic and applied genetic research.

scholarly journals Cover Picture: A PEG-Based Biocompatible Block Catiomer with High Buffering Capacity for the Construction of Polyplex Micelles Showing Efficient Gene Transfer toward Primary Cells (ChemMedChem 4/2006)

ChemMedChem ◽  
2006 ◽  
Vol 1 (4) ◽  
pp. 389-389
Author(s):  
Naoki Kanayama ◽  
Shigeto Fukushima ◽  
Nobuhiro Nishiyama ◽  
Keiji Itaka ◽  
Woo-Dong Jang ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Buffering Capacity ◽  
Primary Cells ◽  
Efficient Gene
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