Four New Eclipsing Binary Systems in the TESS field: CD-34 13220, HD 295082, TYC 6484–426–1 and TYC 6527–2310–1

We present the first evidence for the binarity of four targets in the TESS field. The temperatures are estimated by SED analysis and the orbital periods are determined. The TESS light curves of the systems are analyzed and the orbital and the absolute parameters are derived. The targets are also compared to well-studied binary systems with the same morphological type and their evolutionary states are discussed. Our results indicate that the stars belong to the class of eclipsing detached binary systems.

KẾT QUẢ SỚM PHẪU THUẬT CẮT U MÔ ĐỆM (GIST) DẠ DÀY TẠI KHOA NGOẠI - BỆNH VIỆN BẠCH MAI

Mục tiêu: mô tả một số đặc điểm lâm sàng, cận lâm sàng và kết quả phẫu thuật u mô đệm của dạ dày. Đối tượng và phương pháp nghiên cứu: mô tả loạt ca bệnh, những trường hợp u mô đệm của dạ dày được phẫu thuật tại bệnh viện Bạch Mai từ 2016 đên 2019. Kết quả: 50 bệnh nhân với tuổi trung bình 56,3±12,6 và tỉ lệ nam/nữ là 0,79. Đau bụng là triệu chứng thường gặp (94%), nội soi thấy có loét trên u 52% và vị trí u chủ yếu ở hang vị và thân vị (74%); cắt lớp vi tính thấy kích thước u 9,2±9,0 cm, bờ đều 88%, ngấm thuốc mạnh 72%; hai dấu ấn CD117 và CD 34 thấy 100%. Cắt dạ dày hình chêm là kỹ thuật dùng nhiều nhất 76%; thời gian trung tiện 1,9±0,7 ngày, rút sonde dạ dày 2,5±1,3 ngày, cho ăn trở lại 3,2±1,2 ngày, nằm viện sau mổ trung bình 6,2 ngày. Tai biến chảy máu trong mổ 2 (4,0%), biến chứng nhiễm trùng vết mổ 4 (8,0%). Kết luận: Chẩn đoán bệnh chủ yếu dựa vào nội soi, chẩn đoán hình ảnh và xác định bằng hóa mô miễn dịch. Phẫu thuật có kết quả sớm tốt, song cần theo dõi lâu dài thêm.

Uso de laser de diodo na dissecção da veia safena para cirurgia de revascularização do miocárdio Brasília

A cirurgia de revascularização de miocárdio foi desenvolvida para tratamento de doença arterial coronariana (DAC), em meados nos anos 60, com enxerto, inicialmente de veia safena e, posteriormente, com a artéria torácica interna (ATI), que mudou por completo o tratamento de DAC e o prognóstico dos pacientes. Para ter o máximo em resultados, a qualidade do enxerto é de extrema importância, incluindo as técnicas para dissecção. Muitas técnicas para diminuir a lesão tecidual tem sido estudadas na busca de aumentar a patência vascular. O uso de laser de diodo é uma alternativa para a extração da veia safena. Objetivo: Comparar o grau de lesão tecidual provocado pelo laser de diodo na dissecção da veia safena e da ATI com o grau de lesão tecidual provocado pelo uso do eletrocautério. Metodologia: É um estudo prospectivo e randomizado que comparou o uso de laser de diodo e o uso de eletrocautério na dissecção da veia safena e da ATI em cirurgias de revascularização do miocárdio no período entre janeiro e junho de 2019. A amostra foi composta de 18 pacientes divididos em quatro grupos: Grupo A1: uso de laser de diodo na dissecção de ATI. Grupo A2: uso de laser de diodo na dissecção de veia safena. Grupo B1: uso de eletrocautério na dissecção de ATI. Grupo B2: uso de eletrocautério na dissecção de veia safena. Foram retirados fragmentos da veia safena e da ATI para um estudo de imuno-histoquímica com marcadores CD-31 e CD-34 para avaliação de processo inflamatório, que diminui a patência do enxerto no médio e longo prazo. Resultados: O estudo foi realizado em dezoito pacientes divididos em quatro grupos. O Grupo A1 é o grupo teste (laser de diodo) de dissecção de ATI e foi formado por 5 pacientes (4 do sexo masculino e 1 do sexo feminino) com idade entre 57 e 71 anos (média 62,6 + 6,11). O Grupo A2 é o grupo teste (laser de diodo) de dissecção de veia safena e foi formado por 3 pacientes (1 do sexo masculino e 2 do sexo feminino) com idade entre 61 e 80 anos (média 68,3 + 8,34). O Grupo B1 é o grupo controle (eletrocautério) de dissecção de ATI e foi formado por 5 pacientes (2 do sexo masculino e 3 do sexo feminino) com idade entre 44 e 80 anos (média 61,8 anos ±13,28) e o Grupo B2 é o grupo controle (eletrocautério) de dissecção de veia safena e foi formado por 5 pacientes (1 do sexo masculino e 4 do sexo feminino) com idade entre 44 e 71 anos (média 58,25 + 9,69). Conclusão: Não foi observado variação de lesão tecidual entre o uso de laser de diodo e eletrocautério, mostrando ser factível o uso do laser de diodo para extração de enxerto de veia safena na utilização para cirurgia de revascularização do miocárdio.

Effectiveness of Artemisia Vulgaris as Supplementation Against Chemotherapy of Mammae Adenocarcinoma to Reduce Expression of NF-κB and CD 34 (Study in C3H Mice Given Chemotherapy Adriamycin - Cyclophosphamide)

Intoduction: Breast cancer is a significant healthcare problem worldwide. Surgery remains the treatment of choice combined with other modalities such as chemotherapy, radiation, and immunotherapy such as Artemisia vulgaris (AV). Selective cytotoxicity of AV is intended as a supplementation to Adriamycin-Cyclophosphamide, improving the response rate of chemotherapy in adenocarcinoma mammae. Method: This study used a "Post-test only control group design" on 24 females C3H mice that were randomly selected and divided into four groups: group K (control), P1 (chemotherapy), P2 (extract), and P3 (combination). Adenocarcinoma mammae come from the inoculation of donor mice. Chemotherapy of Adriamycin 60 mg / m 2 and Cyclophosphamide 600 mg / m 2 were given in two cycles. AV 13 mg (0.2 ml) was given once daily orally. NF-κB expression and CD34were evaluated using imunohistochemical staining. Result: The expression of NF-κB and microvascular density of CD 34 were obtained in groups of K, P1, P2, P3 Statistical analysis showed significant decrease in the expression of NF-κB between groups K and P1, P2, P3. Correlation analysis between NF-κB expression with CD 34 was found to have significant correlation (p = 0,039 and r = 0,897). Conclusion:Artemisia vulgaris can reduce angiogenesis by decreasing NF-κB expression and the microvascular density CD34 of adenocarcinoma mammae of C3H mice treated with Adriamycin-Cyclophosphamide chemotherapy and can improve the effectivity.

Development of hematopoietic syndrome mice model for localized radiation exposure

Current models to study the hematopoietic syndrome largely rely on the uniform whole-body exposures. However, in the radio-nuclear accidents or terrorist events, exposure can be non-uniform. The data available on the non-uniform exposures is limited. Thus, we have developed a mice model for studying the hematopoietic syndrome in the non-uniform or partial body exposure scenarios using the localized cobalt60 gamma radiation exposure. Femur region of Strain ‘A’ male mice was exposed to doses ranging from 7 to 20 Gy. The 30 day survival assay showed 19 Gy as LD100 and 17 Gy as LD50. We measured an array of cytokines and important stem cell markers such as IFN-γ, IL-3, IL-6, GM-CSF, TNF-α, G-CSF, IL-1α, IL-1β, CD 34 and Sca 1. We found significant changes in IL-6, GM-CSF, TNF-α, G-CSF, and IL-1β levels compared to untreated groups and amplified levels of CD 34 and Sca 1 positive population in the irradiated mice compared to the untreated controls. Overall, we have developed a mouse model of the hematopoietic acute radiation syndrome that might be useful for understanding of the non-uniform body exposure scenarios. This may also be helpful in the screening of drugs intended for individuals suffering from radiation induced hematopoietic syndrome.

Expression of CD34 and α-SMA Markers in Oral Squamous Cell Carcinoma Differentiation. A Histological and Histo-Chemical Study

To reduce morbidity and mortality rates of OSCC cases, early diagnosis, assessment of behavior and prognostic estimates are vital. This study analyzed the expression of CD34 and alpha smooth muscle actin (α-SMA) in OSCC, to establish their significance in diagnosis and prognosis. Primary cases of OSCC, diagnosed with excisional biopsy at multiple cancer treatment centers, were included. Tissue sections were embedded and stained with H & E for histological differentiation and invasion of tumor vessel. Immunohistochemistry was performed using antibodies against CD34 and α-SMA. The chi-square and Pearson correlation coefficient (r) tests were applied for data analysis. Eighty patients with fifty males (62.5%) and thirty females (37.5%) and mean age of 45 ± 14.1 years were evaluated. Buccal mucosa was the most common site for OSCC lesions [36 (45%)]; 47.5% of lesions were moderately differentiated and 33.8% were well-differentiated lesions. Invasion of tumor vessels was observed in 35% of specimens. A significant association was seen between CD34 expression and histological grading of OSCC (p < 0.002). Among all poorly differentiated OSCC specimens, expression of CD 34 was low and α-SMA was high. CD 34 is a critical prognostic factor in OSCC diagnosis and increased α-SMA-positive myofibroblasts may indicate aggressive OSCC behavior.

Clinical and Immunomorphological Features of Liver Damage in Severe Preeclampsia

Background.Liver diseases associated with pregnancy are recorded in 0.73% of pregnant women, often accompanied by the development of hepatic dysfunction/insufficiency, and are the cause of increased morbidity and mortality in both mother and child. A pathomorphological study helps to understand the pathophysiology of severe liver damage in preeclampsia, and to optimize the management of such patients.Aims to study the clinical, morphological and immunohistochemical features of liver tissue lesions in the most severe forms of preeclampsia and eclampsia, which ended in death.Methods.Autopsy material analysis of 10 patients who died from preeclampsia, eclampsia and their complications (main group) and 3 patients who died from other causes (comparison group). Pathomorphological and immunohistochemical studies of organs and tissues (in particular, liver tissue) were performed using a marker of neurons and neuroendocrine cells -NSE and a marker of endotheliocytes CD-34.Results.An immunohistochemical study with a CD-34 endotheliocyte marker in the main group revealed a vascularization deficiency in the 2nd and 3rd zone of hepatic acini, there were also foci of necrosis. Such changes indicate deep and prolonged hypoperfusion. The use of the NSE marker in the group of patients who died from preeclampsia/eclampsia revealed a sharp increase in Kupffer cells in the first and second zones of acini with pronounced immunoexpression of NSE in the nuclei and cytoplasm of these cells, which indicates severe hepatic dysfunction (in particular, impaired detoxification and elimination functions of the liver). At the same time, only 3 out of 10 women in the main group are clinically registered with HELLP syndrome, while the rest had signs of multiple organ (including acute liver) failure.Conclusions.The clinical symptoms of liver damage, including those with severe preeclampsia, arise, as a rule, already against the background of severe morphological changes in its tissue and, as a rule, indicate functional decompensation. Liver immunology remains little studied, which requires further research on this problem.