A Combined In Vitro and In Vivo Assessment of the Safety of the Yeast Strains Kluyveromyces marxianus A4 and A5 Isolated from Korean Kefir

Abstract Objective Endothelin-1 plays an important role in the pathogenesis of severe pulmonary hypertension. The + 139 ‘A’, adenine insertion variant in 5′UTR of edn1 gene has been reported to be associated with increased expression of Endothelin-1 in vitro. The aim of present study was to explore the association of this variant with the circulating levels of Endothelin-1 in vivo using archived DNA and plasma samples from 38 paediatric congenital heart disease (cyanotic and acyanotic) patients with severe pulmonary hypertension. Results The plasma Endothelin-1 levels were highly varied ranging from 1.63 to75.16 pg/ml. The + 139 ‘A’ insertion variant in 5′UTR of edn1 was seen in 8 out of 38 cases with only one acyanotic sample demonstrating homozygosity of inserted ‘A’ allele at + 139 site (4A/4A genotype). The plasma Endothelin-1 levels in children with homozygous variant 3A/3A genotype were comparable in cyanotic and acyanotic groups. Lone 4A/4A acyanotic sample had ET-1 levels similar to the median value of ET-1 associated with 3A/3A genotype and was absent in cyanotic group presumably due to deleterious higher ET-1 levels. The discussed observations, limited by the small sample size, are suggestive of homozygous adenine insertion variant posing a risk in cyanotic babies with Severe Pulmonary Hypertension.

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A new dual function orodissolvable/dispersible meclizine HCL tablet to challenge patient inconvenience: in vitro evaluation and in vivo assessment in human volunteers

Drug Delivery and Translational Research ◽

10.1007/s13346-020-00889-z ◽

2021 ◽

Author(s):

Alaa Y. Darwesh ◽

Marwa S. El-Dahhan ◽

Mahasen M. Meshali

Keyword(s):

Dual Function ◽

In Vitro Evaluation ◽

Human Volunteers ◽

In Vivo Assessment

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In-vivo assessment of in-vitro killing patterns of Pseudomonas aeruginosa

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/15.suppl_a.201 ◽

1985 ◽

Vol 15 (suppl A) ◽

pp. 201-206 ◽

Cited By ~ 17

Author(s):

A. U. Gerber ◽

C. Feller-Segessenmann

Keyword(s):

Pseudomonas Aeruginosa ◽

In Vivo Assessment

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Toxicity of citrate-capped AuNPs: an in vitro and in vivo assessment

Journal of Nanoparticle Research ◽

10.1007/s11051-011-0590-x ◽

2011 ◽

Vol 13 (12) ◽

pp. 6821-6835 ◽

Cited By ~ 32

Author(s):

Stefania Sabella ◽

Virgilio Brunetti ◽

Giuseppe Vecchio ◽

Antonio Galeone ◽

Gabriele Maiorano ◽

...

Keyword(s):

In Vivo Assessment

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Formulation development and in vitro–in vivo assessment of protransfersomal gel of anti-resorptive drug in osteoporosis treatment

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2021.121060 ◽

2021 ◽

pp. 121060

Author(s):

Suman Gyanewali ◽

Prashant Kesharwani ◽

Afsana ◽

Farhan Jalees Ahmad ◽

Ritu Trivedi ◽

...

Keyword(s):

Osteoporosis Treatment ◽

Formulation Development ◽

In Vivo Assessment

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In Vitro Probitotic Evaluation of Saccharomyces boulardii with Antimicrobial Spectrum in a Caenorhabditis elegans Model

Foods ◽

10.3390/foods10061428 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1428

Author(s):

Ramachandran Chelliah ◽

Eun-Ji Kim ◽

Eric Banan-Mwine Daliri ◽

Usha Antony ◽

Deog-Hwan Oh

Keyword(s):

Pancreatic Enzyme ◽

Saccharomyces Boulardii ◽

Antimicrobial Spectrum ◽

Antimicrobial Efficiency ◽

Heat Stable ◽

Yeast Strains ◽

Probiotic Yeast ◽

Probiotic Strains

In the present study, we screened for potential probiotic yeast that could survive under extreme frozen conditions. The antimicrobial and heat-stable properties of the isolated yeast strains Saccharomyces boulardii (S. boulardii) (KT000032, KT000033, KT000034, KT000035, KT000036, and KT000037) was analyzed and compared with commercial probiotic strains. The results revealed that the tested S. boulardii KT000032 strain showed higher resistance to gastric enzymes (bile salts, pepsin, and pancreatic enzyme) at low pH, with broad antibiotic resistance. In addition, the strain also showed efficient auto-aggregation and co-aggregation abilities and efficient hydrophobicity in the in-vitro and in-vivo C. elegens gut model. Further, the KT000032 strain showed higher antimicrobial efficiency against 13 different enteropathogens and exhibited commensal relationships with five commercial probiotic strains. Besides, the bioactive compounds produced in the cell-free supernatant of probiotic yeast showed thermo-tolerance (95 °C for two hours). Furthermore, the thermo-stable property of the strains will facilitate their incorporation into ready-to-eat food products under extreme food processing conditions.

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