Development and characterization of sixteen microsatellite loci for Geosmithia morbida, the causal agent of thousand canker disease in black walnut (Juglans nigra)

2011 ◽  
Vol 4 (2) ◽  
pp. 287-289 ◽  
Author(s):  
Denita Hadziabdic ◽  
Phillip A. Wadl ◽  
Lisa M. Vito ◽  
Sarah L. Boggess ◽  
Brian E. Scheffler ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Black Walnut ◽  
Causal Agent ◽  
Juglans Nigra ◽  
Canker Disease ◽  
Geosmithia Morbida

Genetic differentiation and spatial structure of Geosmithia morbida, the causal agent of thousand cankers disease in black walnut (Juglans nigra)

2013 ◽  
Vol 60 (2) ◽  
pp. 75-87 ◽  
Author(s):  
Denita Hadziabdic ◽  
Lisa M. Vito ◽  
Mark T. Windham ◽  
Jay W. Pscheidt ◽  
Robert N. Trigiano ◽  
...  
Keyword(s):  
Genetic Differentiation ◽  
Spatial Structure ◽  
Black Walnut ◽  
Causal Agent ◽  
Juglans Nigra ◽  
Geosmithia Morbida ◽  
Thousand Cankers Disease

scholarly journals Virulence of Genetically Distinct Geosmithia morbida Isolates to Black Walnut and Their Response to Coinoculation with Fusarium solani

Plant Disease ◽  
2017 ◽  
Vol 101 (1) ◽  
pp. 116-120 ◽  
Author(s):  
Rachael A. Sitz ◽  
Emily K. Luna ◽  
Jorge Ibarra Caballero ◽  
Ned A. Tisserat ◽  
Whitney S. Cranshaw ◽  
...  
Keyword(s):  
Disease Severity ◽  
Fusarium Solani ◽  
Black Walnut ◽  
Causal Agent ◽  
Geosmithia Morbida ◽  
Thousand Cankers Disease ◽  
Synergistic Response

Geosmithia morbida is well documented as the causal agent of thousand cankers disease of black walnut trees. However, it is not well understood how G. morbida strains differ in virulence and how their interactions with co-occurring pathogens contribute to disease severity. In this study, we systematically investigated virulence of genetically distinct G. morbida strains. Overall, we found varying degrees of virulence, although differences were not related to genetic groupings. Furthermore, the pathogen Fusarium solani is also commonly isolated from thousand canker-diseased trees. The degree of disease contribution from F. solani is unknown, along with interactions it may have with G. morbida. This research shows that coinoculation with these pathogens does not yield a synergistic response.

Isolation and characterization of microsatellite loci in Colletotrichum acutatum, the causal agent of postbloom fruit drop on citrus

2011 ◽  
Vol 3 (4) ◽  
pp. 651-654 ◽  
Author(s):  
M. B. Ciampi ◽  
C. Baldauf ◽  
B. B. Z. Vigna ◽  
A. P. Souza ◽  
M. B. Spósito ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Causal Agent ◽  
Colletotrichum Acutatum ◽  
Fruit Drop ◽  
Isolation And Characterization

scholarly journals Effect of Geosmithia morbida Isolate and Temperature on Canker Development in Black Walnut

2012 ◽  
Vol 13 (1) ◽  
pp. 11 ◽  
Author(s):  
Emily Freeland ◽  
Whitney Cranshaw ◽  
Ned Tisserat
Keyword(s):  
Black Walnut ◽  
Juglans Nigra ◽  
Geosmithia Morbida ◽  
Rdna Its ◽  
Pityophthorus Juglandis ◽  
Thousand Cankers Disease ◽  
One Year ◽  
Old Trees ◽  
Canker Development ◽  
Walnut Twig Beetle

Thousand cankers disease of black walnut (Juglans nigra) is the result of aggressive feeding by the walnut twig beetle (Pityophthorus juglandis) and extensive cankering around beetle galleries caused by the fungus Geosmithia morbida. We developed a consistent, reproducible inoculation technique to screen black walnut trees for their reaction to canker development following inoculation with G. morbida. Canker areas in one-year-old trees were not affected by the location on the stem that inoculations were made. Differences in aggressiveness of G. morbida isolates, representing different rDNA ITS haplotype groups, to black walnut were observed in some experiments. However, these differences were small and evidence indicates that a single, highly aggressive haplotype is not responsible for the current TCD epidemic. Cankers formed in black walnut at all temperatures tested, but they were consistently smaller at 32/20°C day/night temperatures compared to 25/20°C. Although G. morbida is thermotolerant, higher temperatures may not enhance canker development. Accepted for publication 1 May 2012. Published 18 June 2012.

scholarly journals Identification and Characterization of Nectria pseudotrichia Associated with Camellia Canker Disease in China

Forests ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 29
Author(s):  
Xiansheng Geng ◽  
Ying Liu ◽  
Jiyuan Li ◽  
Zhihong Li ◽  
Jinping Shu ◽  
...  
Keyword(s):  
Southern China ◽  
Disease Incidence ◽  
Economic Value ◽  
Causal Agent ◽  
Camellia Japonica ◽  
Canker Disease ◽  
Native Tree ◽  
Native Tree Species ◽  
Identification And Characterization

Camellia japonica is a native tree species with high economic value that is widely cultivated in southern China. In recent years, canker disease has been observed in camellia plantations in Zhejiang Province, China, with the disease incidence rate in some plantations exceeding 20%. Canker disease severely affects the trunks and branches of C. japonica in China, but the causal agent has not yet been identified. In this study, the pathogen was isolated from infected C. japonica tissues through a conventional tissue isolation approach. Species identification was conducted using morphological methods combined with multilocus phylogenetic analysis. Pathogenicity was tested based on Koch’s postulates. The results showed that the pathogen could be isolated from the diseased bark of C. japonica ‘Hongluzhen’. The pathogen was identified as Nectria pseudotrichia based on morphological, cultural, and molecular traits. The inoculation of the pathogen into C. japonica ‘Hongluzhen’ caused necrotic lesions on healthy seedlings, and the fungus N. pseudotrichia could be re-isolated from such lesions. Therefore, N. pseudotrichia is the causal agent of canker disease affecting C. japonica in China.

Purification and Characterization of a Black Walnut (Juglans nigra) Allergen, Jug n 4

2017 ◽  
Vol 65 (2) ◽  
pp. 454-462 ◽  
Author(s):  
Yu-Zhu Zhang ◽  
Wen-Xian Du ◽  
Yuting Fan ◽  
Jiang Yi ◽  
Shu-Chen Lyu ◽  
...  
Keyword(s):  
Black Walnut ◽  
Juglans Nigra ◽  
Purification And Characterization

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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