scholarly journals Identification and Characterization of Nectria pseudotrichia Associated with Camellia Canker Disease in China

Forests ◽  
2021 ◽  
Vol 13 (1) ◽  
pp. 29
Author(s):  
Xiansheng Geng ◽  
Ying Liu ◽  
Jiyuan Li ◽  
Zhihong Li ◽  
Jinping Shu ◽  
...  

Camellia japonica is a native tree species with high economic value that is widely cultivated in southern China. In recent years, canker disease has been observed in camellia plantations in Zhejiang Province, China, with the disease incidence rate in some plantations exceeding 20%. Canker disease severely affects the trunks and branches of C. japonica in China, but the causal agent has not yet been identified. In this study, the pathogen was isolated from infected C. japonica tissues through a conventional tissue isolation approach. Species identification was conducted using morphological methods combined with multilocus phylogenetic analysis. Pathogenicity was tested based on Koch’s postulates. The results showed that the pathogen could be isolated from the diseased bark of C. japonica ‘Hongluzhen’. The pathogen was identified as Nectria pseudotrichia based on morphological, cultural, and molecular traits. The inoculation of the pathogen into C. japonica ‘Hongluzhen’ caused necrotic lesions on healthy seedlings, and the fungus N. pseudotrichia could be re-isolated from such lesions. Therefore, N. pseudotrichia is the causal agent of canker disease affecting C. japonica in China.

Plant Disease ◽  
2019 ◽  
Vol 103 (2) ◽  
pp. 249-258 ◽  
Author(s):  
Miloš Stevanović ◽  
Danijela Ristić ◽  
Svetlana Živković ◽  
Goran Aleksić ◽  
Ivana Stanković ◽  
...  

Blackberry cane diseases with the symptoms of necrosis, canker, and wilting are caused by several fungi worldwide. Surveys conducted from 2013 to 2016 in Serbia revealed the occurrence of Gnomoniopsis idaeicola, the causal agent of cane canker and wilting, which was found to be distributed in almost half of the surveyed orchards, in three blackberry cultivars, and with disease incidence of up to 80%. Wide distribution and high disease incidence suggest that G. idaeicola has been present in Serbia for some time. Out of 427 samples, a total of 65 G. idaeicola isolates were obtained (isolation rate of 34.19%). Reference isolates, originating from different localities, were conventionally and molecularly identified and characterized. G. idaeicola was detected in single and mixed infections with fungi from genera Paraconiothyrium, Colletotrichum, Diaporthe, Botryosphaeria, Botrytis, Septoria, Neofusicoccum, and Discostroma, and no diagnostically specific symptoms could be related directly to the G. idaeicola infection. In orchards solely infected with G. idaeicola, blackberry plant mortality was up to 40%, and yield loses were estimated at 50%. G. idaeicola isolates included in this study demonstrated intraspecies diversity in morphological, biological, pathogenic, and molecular features, which indicates that population in Serbia may be of different origin. This is the first record of a massive outbreak of G. idaeicola infection, illustrating its capability of harmful influence on blackberry production. This study represents the initial step in studying G. idaeicola as a new blackberry pathogen in Serbia, aiming at developing efficient control measures.


2012 ◽  
Vol 364 (1-2) ◽  
pp. 207-218 ◽  
Author(s):  
Faming Wang ◽  
Weixing Zhu ◽  
Bi Zou ◽  
Deborah A. Neher ◽  
Shenglei Fu ◽  
...  

Sugar Tech ◽  
2019 ◽  
Vol 22 (1) ◽  
pp. 105-111
Author(s):  
Tri Maryono ◽  
Ani Widiastuti ◽  
Rudi Hari Murti ◽  
Achmadi Priyatmojo

Plant Disease ◽  
2018 ◽  
Vol 102 (7) ◽  
pp. 1283-1289 ◽  
Author(s):  
Jianyou Mo ◽  
Guang Zhao ◽  
Qili Li ◽  
Ghulam S. Solangi ◽  
Lihua Tang ◽  
...  

Mango (Mangifera indica) is widely grown across southern China, especially in the provinces of Guangxi, Hainan, Yunnan, Sichuan, and Taiwan. Guangxi itself has over 86,667 ha of mango production. The purpose of this study was to identify Colletotrichum species associated with mango in different parts of Guangxi and examine their pathogenicity on leaves and fruits of mango in vitro. Diseased leaves were collected from 25 mango orchards in different areas of Guangxi province. Sixty-five isolates were obtained from mango leaves with anthracnose symptoms, and these were further characterized based on morphology and DNA sequencing. Twenty-nine isolates from different areas were selected for sequencing and analyses of the internal transcribed spacer region, glyceraldehyde-3-phosphate dehydrogenase, partial actin, β-tubulin, and chitin synthase genomic regions. The most common fungal isolates were these three species: Colletotrichum asianum, C. fructicola, and C. siamense. C. asianum was the most common and widely distributed in Guangxi (51.7%), followed by C. fructicola (37.9%) and C. siamense (10.2%), both found in Tiandong, Tianyang, and Wuming counties. There was no evidence of geographical specialization of the different species. Pathogenicity assays showed that all isolates were pathogenic to mango leaves and fruit (cultivar Tainong). No relationship was found between origin of isolates and their virulence. This is the first description of C. asianum, C. fructicola, and C. siamense as causal agents of mango leaf anthracnose from Guangxi province, China.


2017 ◽  
Vol 42 (4) ◽  
pp. 284-290 ◽  
Author(s):  
Pablo E. Grijalba ◽  
Hemilse E. Palmucci ◽  
Eduardo Guillin

2019 ◽  
Vol 8 (2) ◽  
pp. 137
Author(s):  
I GUSTI AGUNG BAGUS SURADARMA ◽  
GEDE WIJANA ◽  
I NYOMAN SUTEDJA

Identification and Characterization of Fruit Genetic Resources in Buleleng Regency. Fruits have great potential to be developed both to meet the needs of domestic and foreign demand, because the fruits is a source of supply of vitamins, minerals, and fiber needed in the daily menu. Several types of fruit are also required as spa, medicines, and as a means of upakara. One of the prospective development of fruits in Bali is Buleleng. Types of fruit in Buleleng are grape, mango, durian, rambutan and banana. Until now there has been generally identified genetic resources fruits in Buleleng. This study aims to identify, profile and draw up a map of the geographic distribution of genetic resources of fruits in Buleleng. Implementation of the study consists of four activities, namely: secondary data collection, survey of the types of genetic resources and distribution, identification of morphological and agronomic characters of each type of genetic resources and the growing environment and the benefits of fruits. The seeded fruit is determined by Location Quotion (LQ). Based on the observations in the field, Buleleng has a wide range of genetic resources. In a direct observation to the field there are 32 kinds of fruit and 31 sub-types of fruit. Fruits are grouped based on the economic value and their role, including: commercial fruit, rare fruit, fruit for upakara and fruit for medicine. Based on the calculation of LQ method, Buleleng has four featured i.e. avocado, grape, mango and rambutan (LQ>1).


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