Investigating the role of carbohydrate-binding module 34 in cyclomaltodextrinase from Geobacillus thermopakistaniensis: structural and functional analyses

3 Biotech ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Iqra Aroob ◽  
Maryam Javed ◽  
Nasir Ahmad ◽  
Mehwish Aslam ◽  
Naeem Rashid
Keyword(s):  
Carbohydrate Binding ◽  
Carbohydrate Binding Module ◽  
Functional Analyses

Hydrolysis of softwood by Aspergillus mannanase: Role of a carbohydrate-binding module

2010 ◽  
Vol 148 (4) ◽  
pp. 163-170 ◽  
Author(s):  
Tuan Anh Pham ◽  
Jean Guy Berrin ◽  
Eric Record ◽  
Kim Anh To ◽  
Jean-Claude Sigoillot
Keyword(s):  
Carbohydrate Binding ◽  
Carbohydrate Binding Module ◽  
Hydrolysis Of

Clostridium thermocellumXyn10B Carbohydrate-Binding Module 22-2:  The Role of Conserved Amino Acids in Ligand Binding†,‡

Biochemistry ◽  
2001 ◽  
Vol 40 (31) ◽  
pp. 9167-9176 ◽  
Author(s):  
Hefang Xie ◽  
Harry J. Gilbert ◽  
Simon J. Charnock ◽  
Gideon J. Davies ◽  
Michael P. Williamson ◽  
...  
Keyword(s):  
Amino Acids ◽  
Ligand Binding ◽  
Carbohydrate Binding ◽  
Carbohydrate Binding Module ◽  
Conserved Amino Acids

The role of carbohydrate-binding module (CBM) repeat of a multimodular xylanase (XynX) from Clostridium thermocellum in cellulose and xylan binding

2010 ◽  
Vol 48 (6) ◽  
pp. 856-861 ◽  
Author(s):  
Thangaswamy Selvaraj ◽  
Sung Kyum Kim ◽  
Yong Ho Kim ◽  
Yu Seok Jeong ◽  
Yu-Jeong Kim ◽  
...  
Keyword(s):  
Clostridium Thermocellum ◽  
Carbohydrate Binding ◽  
Carbohydrate Binding Module

scholarly journals Exo-exo synergy between Cel6A and Cel7A fromHypocrea jecorina: Role of carbohydrate binding module and the endo-lytic character of the enzymes

2017 ◽  
Vol 114 (8) ◽  
pp. 1639-1647 ◽  
Author(s):  
Silke F. Badino ◽  
Stefan J. Christensen ◽  
Jeppe Kari ◽  
Michael S. Windahl ◽  
Søren Hvidt ◽  
...  
Keyword(s):  
Carbohydrate Binding ◽  
Carbohydrate Binding Module

scholarly journals A carbohydrate-binding family 48 module enables feruloyl esterase action on polymeric arabinoxylan

2019 ◽  
Vol 294 (46) ◽  
pp. 17339-17353 ◽  
Author(s):  
Jesper Holck ◽  
Folmer Fredslund ◽  
Marie S. Møller ◽  
Jesper Brask ◽  
Kristian B. R. M. Krogh ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Surface Plasmon Resonance ◽  
Feruloyl Esterase ◽  
Carbohydrate Binding ◽  
Carbohydrate Binding Module ◽  
Surface Plasmon Resonance Analysis ◽  
Resonance Analysis ◽  
Feruloyl Esterases ◽  
Folded Proteins

Feruloyl esterases (EC 3.1.1.73), belonging to carbohydrate esterase family 1 (CE1), hydrolyze ester bonds between ferulic acid (FA) and arabinose moieties in arabinoxylans. Recently, some CE1 enzymes identified in metagenomics studies have been predicted to contain a family 48 carbohydrate-binding module (CBM48), a CBM family associated with starch binding. Two of these CE1s, wastewater treatment sludge (wts) Fae1A and wtsFae1B isolated from wastewater treatment surplus sludge, have a cognate CBM48 domain and are feruloyl esterases, and wtsFae1A binds arabinoxylan. Here, we show that wtsFae1B also binds to arabinoxylan and that neither binds starch. Surface plasmon resonance analysis revealed that wtsFae1B's Kd for xylohexaose is 14.8 μm and that it does not bind to starch mimics, β-cyclodextrin, or maltohexaose. Interestingly, in the absence of CBM48 domains, the CE1 regions from wtsFae1A and wtsFae1B did not bind arabinoxylan and were also unable to catalyze FA release from arabinoxylan. Pretreatment with a β-d-1,4-xylanase did enable CE1 domain-mediated FA release from arabinoxylan in the absence of CBM48, indicating that CBM48 is essential for the CE1 activity on the polysaccharide. Crystal structures of wtsFae1A (at 1.63 Å resolution) and wtsFae1B (1.98 Å) revealed that both are folded proteins comprising structurally-conserved hydrogen bonds that lock the CBM48 position relative to that of the CE1 domain. wtsFae1A docking indicated that both enzymes accommodate the arabinoxylan backbone in a cleft at the CE1–CBM48 domain interface. Binding at this cleft appears to enable CE1 activities on polymeric arabinoxylan, illustrating an unexpected and crucial role of CBM48 domains for accommodating arabinoxylan.

scholarly journals Structural and Functional Analyses of Carbohydrate Binding Module Family 36 of Xylanase from Paenibacillus Campinasensis

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Hao‐Ting Chang ◽  
Yu‐Sheng Wang ◽  
Kai‐Jay Yang ◽  
Yu‐Jen Chen ◽  
Chun‐Han Ko ◽  
...  
Keyword(s):  
Carbohydrate Binding ◽  
Carbohydrate Binding Module ◽  
Functional Analyses

scholarly journals Role of a family 11 carbohydrate-binding module in the function of a recombinant cellulase used to supplement a barley-based diet for broiler chickens

2008 ◽  
Vol 49 (4) ◽  
pp. 446-454 ◽  
Author(s):  
C.I.P.D. Guerreiro ◽  
T. Ribeiro ◽  
P.I.P. Ponte ◽  
M.M.S. Lordelo ◽  
L. Falcão ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Carbohydrate Binding ◽  
Carbohydrate Binding Module ◽  
Recombinant Cellulase
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