Molecular characterization of a wheat -Thinopyrum ponticum partial amphiploid and its derived substitution line for resistance to stripe rust

2011 ◽  
Vol 52 (3) ◽  
pp. 279-285 ◽  
Author(s):  
Li-Jun Hu ◽  
Guang-Rong Li ◽  
Zi-Xian Zeng ◽  
Zhi-Jian Chang ◽  
Cheng Liu ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Stripe Rust ◽  
Substitution Line ◽  
Thinopyrum Ponticum ◽  
Partial Amphiploid

scholarly journals Molecular cytogenetic identification of a wheat – Thinopyrum ponticum substitution line with stripe rust resistance

Genome ◽  
2017 ◽  
Vol 60 (10) ◽  
pp. 860-867 ◽  
Author(s):  
Chen Zhu ◽  
Yanzhen Wang ◽  
Chunhuan Chen ◽  
Changyou Wang ◽  
Aicen Zhang ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Stripe Rust ◽  
Expressed Sequence Tag ◽  
Wheat Breeding ◽  
Substitution Line ◽  
Stripe Rust Resistance ◽  
Fish Analysis ◽  
Thinopyrum Ponticum ◽  
Expressed Sequence

Thinopyrum ponticum (Th. ponticum) (2n = 10x = 70) is an important breeding material with excellent resistance and stress tolerance. In this study, we characterized the derivative line CH1113-B13-1-1-2-1 (CH1113-B13) through cytological, morphological, genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), expressed sequence tag (EST), and PCR-based landmark unique gene (PLUG) marker analysis. The GISH analysis revealed that CH1113-B13 contained 20 pairs of common wheat chromosomes and one pair of JSt genomic chromosomes. Linkage analysis of Th. ponticum using seven EST and seven PLUG markers indicated that the pair of alien chromosomes belonged to the seventh homeologous group. Nulli-tetrasomic and FISH analysis revealed that wheat 7B chromosomes were absent in CH1113-B13; thus, CH1113-B13 was identified as a 7JSt (7B) substitution line. Finally, adult-stage CH1113-B13 exhibited immunity to wheat stripe rust. This substitution line is therefore a promising germplasm resource for wheat breeding.

Cytological and molecular characterization of non-host resistance in Arabidopsis thaliana against wheat stripe rust

2013 ◽  
Vol 62 ◽  
pp. 11-18 ◽  
Author(s):  
Yulin Cheng ◽  
Hongchang Zhang ◽  
Juanni Yao ◽  
Qingmei Han ◽  
Xiaojie Wang ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Molecular Characterization ◽  
Stripe Rust ◽  
Host Resistance ◽  
Wheat Stripe Rust

Molecular characterization of a gene induced during wheat hypersensitive reaction to stripe rust

2011 ◽  
Vol 55 (4) ◽  
Author(s):  
G. Zhang ◽  
Y. -M. Li ◽  
Y. -F. Sun ◽  
J. -M. Wang ◽  
B. Liu ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Stripe Rust ◽  
Hypersensitive Reaction
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