Molecular Characterization of Novel Totivirus-Like Double-Stranded RNAs from Puccinia striiformis f. sp. tritici, the Causal Agent of Wheat Stripe Rust

Wheat stripe rust (yellow rust [Yr]), caused by Puccinia striiformis f. sp. tritici, is an economically important disease of wheat worldwide. Virulence information on P. striiformis f. sp. tritici populations is important to implement effective disease control with resistant cultivars. In total, 235 P. striiformis f. sp. tritici isolates from Algeria, Australia, Canada, Chile, China, Hungary, Kenya, Nepal, Pakistan, Russia, Spain, Turkey, and Uzbekistan were tested on 20 single Yr-gene lines and the 20 wheat genotypes that are used to differentiate P. striiformis f. sp. tritici races in the United States. The 235 isolates were identified as 129 virulence patterns on the single-gene lines and 169 virulence patterns on the U.S. differentials. Virulences to YrA, Yr2, Yr6, Yr7, Yr8, Yr9, Yr17, Yr25, YrUkn, Yr28, Yr31, YrExp2, Lemhi (Yr21), Paha (YrPa1, YrPa2, YrPa3), Druchamp (Yr3a, YrD, YrDru), Produra (YrPr1, YrPr2), Stephens (Yr3a, YrS, YrSte), Lee (Yr7, Yr22, Yr23), Fielder (Yr6, Yr20), Tyee (YrTye), Tres (YrTr1, YrTr2), Express (YrExp1, YrExp2), Clement (Yr9, YrCle), and Compair (Yr8, Yr19) were detected in all countries. At least 80% of the isolates were virulent on YrA, Yr2, Yr6, Yr7, Yr8, Yr17, YrUkn, Yr31, YrExp2, Yr21, Stephens (Yr3a, YrS, YrSte), Lee (Yr7, Yr22, Yr23), and Fielder (Yr6, Yr20). Virulences to Yr1, Yr9, Yr25, Yr27, Yr28, Heines VII (Yr2, YrHVII), Paha (YrPa1, YrPa2, YrPa3), Druchamp (Yr3a, YrD, YrDru), Produra (YrPr1, YrPr2), Yamhill (Yr2, Yr4a, YrYam), Tyee (YrTye), Tres (YrTr1, YrTr2), Hyak (Yr17, YrTye), Express (YrExp1, YrExp2), Clement (Yr9, YrCle), and Compair (Yr8, Yr19) were moderately frequent (>20 to <80%). Virulence to Yr10, Yr24, Yr32, YrSP, and Moro (Yr10, YrMor) was low (≤20%). Virulence to Moro was absent in Algeria, Australia, Canada, Kenya, Russia, Spain, Turkey, and China, but 5% of the Chinese isolates were virulent to Yr10. None of the isolates from Algeria, Canada, China, Kenya, Russia, and Spain was virulent to Yr24; none of the isolates from Algeria, Australia, Canada, Nepal, Russia, and Spain was virulent to Yr32; none of the isolates from Australia, Canada, Chile, Hungary, Kenya, Kenya, Nepal, Pakistan, Russia, and Spain was virulent to YrSP; and none of the isolates from any country was virulent to Yr5 and Yr15. Although the frequencies of virulence factors were different, most of the P. striiformis f. sp. tritici isolates from these countries shared common virulence factors. The virulences and their frequencies and distributions should be useful in breeding stripe-rust-resistant wheat cultivars and understanding the pathogen migration and evolution.

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Canopy Spectral Characterization of Wheat Stripe Rust in Latent Period

Journal of Spectroscopy ◽

10.1155/2015/126090 ◽

2015 ◽

Vol 2015 ◽

pp. 1-11 ◽

Cited By ~ 1

Author(s):

Qi Liu ◽

Yilin Gu ◽

Shuhe Wang ◽

Cuicui Wang ◽

Zhanhong Ma

Keyword(s):

Latent Period ◽

Stripe Rust ◽

Puccinia Striiformis ◽

Spectral Feature ◽

Wheat Stripe Rust ◽

Wheat Diseases ◽

Average Accuracy ◽

Spectral Detection ◽

Accuracy Rates

Stripe rust, caused byPuccinia striiformisf. sp.tritici(Pst), is one of the important wheat diseases worldwide. In this study, the spectral data were collected from wheat canopy during the latent period inoculated with three different concentrations of urediniospores and classification models based on discriminant partial least squares (DPLS) were built to differentiate leaves with and without infection of the stripe rust pathogen. The effects of different spectra features, wavebands, and the number of the samples used in modeling on the performances of the models were assessed. The results showed that, in the spectral region of 325–1075 nm, the model with the spectral feature of 2nd derivative of Pseudoabsorption index had better accuracy than others. The average accuracy rate was 97.28% for the training set and 92.98% for the testing set. In the waveband of 925–1075 nm, the model with the spectral feature of 1st derivative Pseudoabsorption index had better accuracy than other models, and the average accuracy rates were 98.27% and 94.33% for the training and testing sets, respectively. The results demonstrated that wheat stripe rust in latent period can be qualitatively identified based on the canopy spectral detection. Thus, the method can be used for early monitoring of infections of wheat stripe rust.

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Identification of a Novel Alternaria alternata Strain Able to Hyperparasitize Puccinia striiformis f. sp. tritici, the Causal Agent of Wheat Stripe Rust

Frontiers in Microbiology ◽

10.3389/fmicb.2017.00071 ◽

2017 ◽

Vol 8 ◽

Cited By ~ 5

Author(s):

Li Zheng ◽

Jie Zhao ◽

Xiaofei Liang ◽

Gangming Zhan ◽

Shuchang Jiang ◽

...

Keyword(s):

Stripe Rust ◽

Alternaria Alternata ◽

Puccinia Striiformis ◽

Causal Agent ◽

Wheat Stripe Rust

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A Novel Fungal Hyperparasite of Puccinia striiformis f. sp. tritici, the Causal Agent of Wheat Stripe Rust

PLoS ONE ◽

10.1371/journal.pone.0111484 ◽

2014 ◽

Vol 9 (11) ◽

pp. e111484 ◽

Cited By ~ 13

Author(s):

Gangming Zhan ◽

Yuan Tian ◽

Fuping Wang ◽

Xianming Chen ◽

Jun Guo ◽

...

Keyword(s):

Stripe Rust ◽

Puccinia Striiformis ◽

Causal Agent ◽

Wheat Stripe Rust

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Development and molecular characterization of wheat-Aegilops peregrina Introgression Lines with resistance to leaf rust and stripe rust

10.1101/160648 ◽

2017 ◽

Author(s):

Deepika Narang ◽

Satinder Kaur ◽

Jyoti Saini ◽

Parveen Chhuneja

Keyword(s):

Leaf Rust ◽

Molecular Characterization ◽

Stripe Rust ◽

Puccinia Striiformis ◽

Puccinia Triticina ◽

Introgression Lines ◽

Stripe Rust Resistance ◽

Adult Plant ◽

Plant Stage

AbstractA wild non-progenitor species from wheat tertiary gene pool Aegilops peregrina accession pau3519 (UUSS) was used for introgression of leaf rust and stripe rust resistance in bread wheat. It was crossed and backcrossed with hexaploid wheat line Chinese Spring PhI to develop two homozygous BC2F6 wheat-Ae. peregrina introgression lines (ILs) viz. IL pau16058 and IL pau16061 through induced homoeologous recombination. Homozygous lines were screened against six Puccinia triticina and two Puccinia striiformis f. sp. tritici pathotypes at the seedling stage and a mixture of prevalent pathotypes of both rust pathogens at the adult plant stage. IL pau16061 showed resistance to leaf rust only while IL pau16058 was resistant to both leaf and stripe rust pathotypes throughout plant life. Molecular characterization of these ILs aided in defining the introgressed regions. Identification of linked markers with advance genomic technologies will aid in marker assisted pyramiding of alien genes in cultivated wheat background.

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Characterization of Wheat Stripe Rust Resistance Genes in Shaanmai 139

ACTA AGRONOMICA SINICA ◽

10.3724/sp.j.1006.2010.00109 ◽

2010 ◽

Vol 36 (1) ◽

pp. 109-114 ◽

Cited By ~ 8

Author(s):

Hong ZHANG ◽

Zhi-Long REN ◽

Yin-Gang HU ◽

Chang-You WANG ◽

Wan-Quan JI

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Wheat Stripe Rust ◽

Rust Resistance Genes

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Molecular characterization of Prunus necrotic ringspot virus isolated from rose in Brazil

Ciência Rural ◽

10.1590/0103-8478cr20141810 ◽

2015 ◽

Vol 45 (12) ◽

pp. 2197-2200 ◽

Cited By ~ 2

Author(s):

Thor Vinícius Martins Fajardo ◽

Monique Bezerra Nascimento ◽

Marcelo Eiras ◽

Osmar Nickel ◽

Gilvan Pio-Ribeiro

Keyword(s):

Amino Acid ◽

Molecular Characterization ◽

Molecular Analysis ◽

Nucleotide Sequences ◽

Amino Acid Sequences ◽

Causal Agent ◽

Ringspot Virus ◽

Prunus Necrotic Ringspot Virus ◽

First Time

ABSTRACT: There is no molecular characterization of Brazilian isolates of Prunus necrotic ringspot virus (PNRSV), except for those infecting peach. In this research, the causal agent of rose mosaic was determined and the movement (MP) and coat (CP) protein genes of a PNRSV isolate from rose were molecularly characterized for the first time in Brazil. The nucleotide and deduced amino acid sequences of MP and CP complete genes were aligned and compared with other isolates. Molecular analysis of the MP and CP nucleotide sequences of a Brazilian PNRSV isolate from rose and others from this same host showed highest identities of 96.7% and 98.6%, respectively, and Rose-Br isolate was classified in PV32 group.

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