A method for rearing larvae of the parasitoid beetle Dastarcus longulus (Coleoptera: Bothrideridae) using Zophobas atratus (Coleoptera: Tenebrionidae) pupae

2022 ◽  
Author(s):  
Akine Sato ◽  
Kyo Itoyama
Keyword(s):  
Zophobas Atratus ◽  
Coleoptera Tenebrionidae

Zophobas atratus (Fabricius, 1775) – new genus and species of darkling beetles (Coleoptera, Tenebrionidae) for the fauna of Ukraine

2018 ◽  
Vol 14 (1) ◽  
pp. 10-24
Author(s):  
V. N. Fursov ◽  
L. S. Cherney
Keyword(s):  
Life Cycle ◽  
Comparative Analysis ◽  
Sexual Dimorphism ◽  
Antennal Segment ◽  
Laboratory Culture ◽  
Important Species ◽  
Darkling Beetles ◽  
Zophobas Atratus ◽  
Sensory Zone ◽  
Coleoptera Tenebrionidae

Darkling beetle Zophobas atratus (Coleoptera, Tenebrionidae) is recorded here for the first time as a new species for the fauna of Ukraine. Detailed study on morphology of preimaginal stages and biology of this species recently introduced to Ukraine, is given here. Zophobas atratus is an important species being easily reared in laboratory cultures and widely distributed in North and South America, Europe, and Asia. Detailed descriptions of all life stages, including egg, young and older larvae, pupa and adult of Z. atratus are required for further taxonomical study of the genus Zophobas, which isn’t yet definitively established. New identification keys for adults and larvae of the genera of tribe Tenebrionini are presented here, based on a comparative analysis of the taxonomic characters of adults and larvae of Z. atratus and species from the genera Tenebrio and Neatus. Comparative analysis of morphology of larva of Z. atratus and larvae of the tribe Cteniopodini of close subfamily Alleculinae was conducted here. The subfamily Alleculinae previously had the rank of family Alleculidae, but our analysis confirmed the reliability of its current taxonomic position as subfamily. The study of morphology of larvae of 1st and 2nd instars of Z. atratus revealed that they are characterized by special taxonomic structures that are not characteristic for oldest instars of larvae of Z. atratus. These characters include absence of spines on caudal segment, presence of a set of 4 setae at posterior margin of tergites of prothorax, metathorax, and 1st to 8th abdominal segments, strongly convex 2nd antennal segment and sensory zone in the form of an open ring on its apex, and etc. Moreover, structure of antenna of larvae of Z. atratus is similar to that of oldest larvae of most species of darkling beetles of the fauna of Ukraine. The most distinctive features of Z. atratus are: sexual dimorphism in structure of clypeus of adults; filiform sclerotized antenna of larva with a continuous sensory zone at apex of 2nd segment, weakly developed 3rd segment; fusion of sclerotized pleurites of 1st–8th abdominal segments with their tergites; sexual dimorphism in structure of 9th abdominal segment of pupa, and presence of two hooks on apex of its appendages. The data of original study of features of life cycle of Z. atratus are given. The pictures and photos of details of morphology of egg, larvae, pupa and adult of Z. atratus are presented. It was recored that life cycle of Z. atratus from laying of egg to the emergence of adult continues from 169 up to 181 days. Adults lived maximum up to 206 days. Maturation of eggs in female after copulation continues 10-11 days. Stage of egg continues 7 days, larva – up to 151 days, including pre-pupal period from 6 to 22 days, pupa – from 8 to 21 days. Twelve larval stages of Z. atratus were recorded in laboratory culture.

scholarly journals Assessing the Efficiency of Molecular Markers for the Species Identification of Gregarines Isolated from the Mealworm and Super Worm Midgut

2018 ◽  
Vol 6 (4) ◽  
pp. 119 ◽  
Author(s):  
Chiara Nocciolini ◽  
Claudio Cucini ◽  
Chiara Leo ◽  
Valeria Francardi ◽  
Elena Dreassi ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Species Identification ◽  
Alternative Source ◽  
Human Welfare ◽  
Terrestrial Invertebrates ◽  
Life Cycle Stages ◽  
Negative Effect ◽  
Zophobas Atratus ◽  
18S Rdna Gene ◽  
Coleoptera Tenebrionidae

Protozoa, of the taxon Gregarinasina, are a heterogeneous group of Apicomplexa that includes ~1600 species. They are parasites of a large variety of both marine and terrestrial invertebrates, mainly annelids, arthropods and mollusks. Unlike coccidians and heamosporidians, gregarines have not proven to have a negative effect on human welfare; thus, they have been poorly investigated. This study focuses on the molecular identification and phylogeny of the gregarine species found in the midgut of two insect species that are considered as an alternative source of animal proteins for the human diet: the mealworm Tenebrio molitor, and the super-worm Zophobas atratus (Coleoptera: Tenebrionidae). Gregarine specimens were isolated from the gut of both larval and adult stages of T. molitor specimens, as well as from Z. atratus larvae. The morphological analyses were restricted to the identification of the different parasite morpho-types, likely corresponding either to different life-cycle stages or to alternative gregarine species. The samples were also used for the DNA extraction necessary for their genetic characterization. Finally, the efficiency of different molecular markers (i.e., 18S rDNA gene alone or combined with the Internal Transcribed Spacer 1) was assessed when applied either to gregarine species identification and to phylogenetic inference.

Optimal hatching conditions of Zophobas atratus (Coleoptera: Tenebrionidae) eggs under various culture conditions

2021 ◽  
Author(s):  
Kyu-Won Kwak ◽  
Sun Young Kim ◽  
Hyun-Jin Ko ◽  
Kyeong Yong Lee ◽  
Jeong-Hun Song ◽  
...  
Keyword(s):  
Culture Conditions ◽  
Zophobas Atratus ◽  
Coleoptera Tenebrionidae
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