Optimization of process parameters and estimation of kinetic parameters for lactic acid production by Lactobacillus casei MTCC 1423

2018 ◽  
Vol 9 (2) ◽  
pp. 253-266 ◽  
Author(s):  
Avinash Thakur ◽  
Parmjit S. Panesar ◽  
Manohar Singh Saini
Keyword(s):  
Lactic Acid ◽  
Kinetic Parameters ◽  
Process Parameters ◽  
Acid Production ◽  
Lactobacillus Casei ◽  
Lactic Acid Production ◽  
Optimization Of Process Parameters

scholarly journals Parametric Optimization of Lactic Acid Production by Immobilized Lactobacillus casei Using Box-Behnken Design

2018 ◽  
Vol 62 (3) ◽  
pp. 274-285 ◽  
Author(s):  
Avinash Thakur ◽  
Paramjit Singh Panesar ◽  
Manohar Singh Saini
Keyword(s):  
Lactic Acid ◽  
Process Parameters ◽  
Acid Production ◽  
Lactobacillus Casei ◽  
Incubation Temperature ◽  
Lactic Acid Production ◽  
Yield Coefficient ◽  
Box Behnken Design ◽  
Optimization Of Process Parameters ◽  
Quadratic Models

Technological optimization of process parameters posses one of the open challenge for fermentative lactic acid (LA) production. Hence optimization of process parameters viz. sugar concentration, pH, biomass, incubation temperature and incubation time for maximizing fermentative lactic acid production from molasses sugar and corn steep liquor as a low cost carbon and nitrogen source, respectively by immobilized Lactobacillus casei MTCC 1423 cells has been carried out using Box Behnken Design (BBD). By applying multiple regressions on experimental data, quadratic models have been realized, explaining role of each variable and their quadratic interaction on LA production, LA productivity and yield coefficient. Analysis of variance has demonstrated that models are significant. The maximum LA production (132 g/(L fermentor volume) ), LA productivity of 2.36 g/(L×h) and yield coefficient of 0.936 g/(g substrate) have been estimated by the quadratic regression model for optimum process parameters values of sugar concentration (194 g/L), pH (6.85), biomass (310 mg, CDW), incubation temperature (37°C) and incubation time (57 h). The optimization validated experiments had resulted in LA production of 130±2.1 g/(L fermentor volume) ; LA productivity of 2.28±0.037 g/(L×h) and yield coefficient of 0.921±0.003 g/(g substrate) and which are substantially higher than those obtained with free cells of Lb. casei MTCC 1423 (2%, v/v inoculums size) at obtained optimized process parameters values. Thus resulted quadratic models provided an opportunity for scaling up the lactic acid production process and demonstrated the economic potential of using agro industrial waste molasses sugar for lactic acid production by Lb. casei MTCC 1423.

L(+)-Lactic Acid Production Using Lactobacillus Casei in Solid-State Fermentation

2005 ◽  
Vol 27 (21) ◽  
pp. 1685-1688 ◽  
Author(s):  
Pappy John Rojan ◽  
Kesavan Madhavan Nampoothiri ◽  
Athira Syamaprasad Nair ◽  
Ashok Pandey
Keyword(s):  
Lactic Acid ◽  
Solid State ◽  
Solid State Fermentation ◽  
Acid Production ◽  
Lactobacillus Casei ◽  
Lactic Acid Production

Lactic acid production from whey permeate by immobilized Lactobacillus casei

1985 ◽  
Vol 7 (4) ◽  
pp. 164-168 ◽  
Author(s):  
Amita Tuli ◽  
R.P. Sethi ◽  
P.K. Khanna ◽  
S.S. Marwaha ◽  
J.F. Kennedy
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactobacillus Casei ◽  
Lactic Acid Production ◽  
Whey Permeate
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