Development of molecular markers for authentication of the medicinal plant species Patrinia by random amplified polymorphic DNA (RAPD) analysis and multiplex-PCR

2016 ◽  
Vol 57 (2) ◽  
pp. 182-190 ◽  
Author(s):  
Byeong Cheol Moon ◽  
Young Mi Lee ◽  
Wook Jin Kim ◽  
Yunui Ji ◽  
Young Min Kang ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Medicinal Plant ◽  
Multiplex Pcr ◽  
Plant Species ◽  
Rapd Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Medicinal Plant Species

scholarly journals Application of molecular markers in medicinal plant studies

2013 ◽  
Vol 5 (1) ◽  
pp. 80-90 ◽  
Author(s):  
Alireza Pourmohammad
Keyword(s):  
Molecular Markers ◽  
Medicinal Plant ◽  
Plant Species ◽  
Crop Improvement ◽  
World Health ◽  
Medicinal Plant Species ◽  
Conservation Strategies ◽  
Medicinal Species ◽  
Healthcare Needs ◽  
Health Organization

Abstract The World Health Organization has estimated that more than 80% of the world’s population in developing countries depends primarily on herbal medicine for basic healthcare needs. Approximately two thirds of the 50 000 different medicinal plant species in use are collected from the wild and only 10% of medicinal species used commercially are cultivated. DNA-based molecular markers have utility in the fields like taxonomy, physiology, embryology, genetics, etc. DNA-based techniques have been widely used for authentication of plant species of medicinal importance. The geographical conditions affect the active constituents of the medicinal plant and hence their activity profiles. Many researchers have studied geographical variation at the genetic level. Estimates of genetic diversity are also important in designing crop improvement programmes for the management of germplasm and evolving conservation strategies. The DNA-based molecular marker helps in the improvement of medicinal plant species. DNA markers are more reliable because the genetic information is unique for each species and is independent of age, physiological conditions and environmental factors.

Authentication of Akebia quinata DECNE. from its common adulterant medicinal plant species based on the RAPD-derived SCAR markers and multiplex-PCR

2014 ◽  
Vol 37 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Byeong Cheol Moon ◽  
Yunui Ji ◽  
Young Mi Lee ◽  
Young Min Kang ◽  
Ho Kyoung Kim
Keyword(s):  
Medicinal Plant ◽  
Multiplex Pcr ◽  
Plant Species ◽  
Medicinal Plant Species ◽  
Scar Markers

scholarly journals Dormancy break in seeds of "quina" (Strychnos pseudoquina A. St.-Hil.)

2011 ◽  
Vol 13 (4) ◽  
pp. 507-511 ◽  
Author(s):  
J.M. Vasconcelos ◽  
M.A. Rodrigues ◽  
S.C. Vasconcelos Filho ◽  
J.F. Sales ◽  
F.G. Silva ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Medicinal Plant ◽  
Gibberellic Acid ◽  
Plant Species ◽  
Germination Percentage ◽  
Boiling Water ◽  
Medicinal Plant Species ◽  
Distilled Water ◽  
Brazilian Cerrado ◽  
Germination Speed

"Quina" (Strychnos pseudoquina A. St. Hil) is a medicinal plant species from the Brazilian Cerrado. As its seeds show dormancy, they were subjected to the treatments pre-cooling at 5ºC during 7 days, pre-heating at 40ºC during 7 days, pre-soaking in sulfuric acid PA during 5 and 15 min, pre-soaking in boiling water during 5 and 15 min, pre-soaking in 100 and 200 ppm gibberellic acid during 48 h, pre-soaking in distilled water during 24 and 48 h, and mechanical scarification to break dormancy. Counts were daily conducted from the 2nd day after the experiment implementation until the germination stabilization at the 65th day. The germination speed index (GSI) and the germination percentage were evaluated. Germination rates above 96% were reached in seeds pre-soaked in water during 48 h and substrate moistened with water or KNO3.

Sign in / Sign up

Export Citation Format

Share Document