Preparation of Bed Reactor Using Brick Dust Immobilized Starch Phosphorhylase from Potato Tubers (Solanum tuberosum L. var. Kufri Badshah) for Production of Glucose-1-phosphate

2013 ◽  
Vol 36 (2) ◽  
pp. 133-137
Author(s):  
Supriya Gupta ◽  
Sanjay Mohan Gupta ◽  
Anil Kumar
1966 ◽  
Vol 21 (9) ◽  
pp. 879-888 ◽  
Author(s):  
Volkmar Loeschcke ◽  
Hermann Stegemann

Up to 25 proteins have been found in the sap of potato tubers by polyacrylamid electrophoresis. The mode of preparation and concentration of the plant material was investigated as well as the pretreatment of the polyacrylamid gel to avoid undue retardation of the migrating bands.Sulfite was the most active inhibitor of sap-browning compared with ascorbic acid and cysteine. which did not influence the protein pattern except for one line. The juice had to be dialyzed or filtered through Sephadex G 25 to remove an unkown acidic material which interferes with the more acidic proteins and causes spreading and diffusing of bands. Proteins were concentrated by means of Carbowax MW 20000. For best results ionic contaminations inherent in the gels was removed by electrophoresis before applying the proteins or dyes. Very few proteins were affected by decreasing the pore size of the gel. Ammonium sulfate (40% saturation) precipitated mainly those proteins which are similar in electrophoretic behavior to the albumin- and transferrinregion of human plasma.


Plant Biology ◽  
2002 ◽  
Vol 4 (1) ◽  
pp. 77-85 ◽  
Author(s):  
M.-L. Fauconnier ◽  
J. Rojas-Beltrán ◽  
J. Delcarte ◽  
F. Dejaeghere ◽  
M. Marlier ◽  
...  

2000 ◽  
Vol 26 (3) ◽  
pp. 153-160 ◽  
Author(s):  
Yosuke MURATA ◽  
Kevin F. YAPTENCO ◽  
Tomohiro NOGUCHI ◽  
Toshiro SUZUKI ◽  
Hiroaki SATO ◽  
...  

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