Identification of Late Blight Resistance Gene Homologues in Wild Solanum Species

2016 ◽  
Vol 88 (2) ◽  
pp. 789-796
Author(s):  
A. K. Srivastava ◽  
B. P. Singh ◽  
S. K. Kaushik ◽  
V. Bhardwaj ◽  
J. K. Tiwari ◽  
...  
Keyword(s):  
Late Blight ◽  
Resistance Gene ◽  
Late Blight Resistance ◽  
Solanum Species ◽  
Blight Resistance ◽  
Wild Solanum Species ◽  
Wild Solanum

scholarly journals Late Blight Resistance Screening of Major Wild Swedish Solanum Species: S. dulcamara, S. nigrum, and S. physalifolium

2018 ◽  
Vol 108 (7) ◽  
pp. 847-857 ◽  
Author(s):  
Kibrom B. Abreha ◽  
Åsa Lankinen ◽  
Laura Masini ◽  
Sofia Hydbom ◽  
Erik Andreasson
Keyword(s):  
Late Blight ◽  
Phytophthora Infestans ◽  
Quantitative Pcr ◽  
Late Blight Resistance ◽  
Solanum Species ◽  
Blight Resistance ◽  
Resistance Screening ◽  
Wild Solanum Species ◽  
Growth Difference

To understand the contribution of wild Solanum species to the epidemiology of potato late blight in Sweden, we characterized the resistance of the three putative alternative hosts: S. physalifolium, S. nigrum, and S. dulcamara to Phytophthora infestans, the causal agent of late blight. The pathogen sporulated in all 10 investigated S. physalifolium genotypes, suggesting susceptibility (S phenotype). Field-grown S. physalifolium was naturally infected but could regrow, though highly infected genotypes were smaller at the end of the season. In 75 S. nigrum genotypes, there were no symptoms (R phenotype) or a lesion restricted to the point of inoculation (RN phenotype), indicating resistance. In 164 S. dulcamara genotypes, most resistance variability was found within sibling groups. In addition to the three resistance phenotypes (R, RN, and S), in S. dulcamara a fourth new resistance phenotype (SL) was identified with lesions larger than the point of inoculation but without visible sporulation of the pathogen. Quantitative PCR confirmed P. infestans growth difference in RN, SL, and S phenotypes. Thus, in Sweden S. physalifolium is susceptible and could be a player in epidemiology. A limited role of S. dulcamara leaves in the epidemiology of late blight was suggested, since no major symptoms have been found in the field.

scholarly journals Performance of Transgenic Potato Containing the Late Blight Resistance Gene RB

Plant Disease ◽  
2008 ◽  
Vol 92 (3) ◽  
pp. 339-343 ◽  
Author(s):  
Dennis A. Halterman ◽  
Lara Colton Kramer ◽  
Susan Wielgus ◽  
Jiming Jiang
Keyword(s):  
Late Blight ◽  
Resistance Gene ◽  
Late Blight Resistance ◽  
Transgenic Potato ◽  
Field Trials ◽  
Blight Resistance ◽  
Solanum Bulbocastanum ◽  
Wild Potato Species ◽  
Foliar Resistance ◽  
In The Wild

Late blight of potato, caused by Phytophthora infestans, is one of the most devastating diseases of potato. A major late blight resistance gene, called RB, previously was identified in the wild potato species Solanum bulbocastanum through map-based cloning. The full-length gene coding sequence, including the open reading frame and promoter, has been integrated into cultivated potato (S. tuberosum) using Agrobacterium-mediated transformation. RB-containing transgenic plants were challenged with P. infestans under optimal late blight conditions in greenhouse experiments. All transgenic lines containing RB exhibited strong foliar resistance. Field-grown transgenic tubers also were tested for resistance to P. infestans. In contrast to the foliar resistance phenotype, RB-containing tubers did not exhibit increased resistance. Two years of field trials were used to ascertain whether the presence of RB had any effect on tuber yield. We were unable to detect any significant effect on tuber size or yield after addition of the resistance gene to several S. tuberosum cultivars.

scholarly journals A Test of Taxonomic Predictivity: Resistance to Early Blight in Wild Relatives of Cultivated Potato

2008 ◽  
Vol 98 (6) ◽  
pp. 680-687 ◽  
Author(s):  
S. H. Jansky ◽  
R. Simon ◽  
D. M. Spooner
Keyword(s):  
Breeding System ◽  
Early Blight ◽  
Cladistic Analysis ◽  
Solanum Species ◽  
Morphological Data ◽  
Blight Resistance ◽  
Wild Solanum Species ◽  
Group Breeding ◽  
Mold Resistance ◽  
Wild Solanum

Host plant resistance offers an attractive method of control for early blight (caused by the foliar fungus Alternaria solani), a widespread disease that appears annually in potato crops worldwide. We tested the assumed ability of taxonomy to predict the presence of early blight resistance genes in wild Solanum species for which resistance was observed in related species. We also tested associations to ploidy, crossing group, breeding system, and geography. As in a prior study of Sclerotinia sclerotiorum (white mold) resistance, tremendous variation for resistance to early blight was found to occur within and among species. There was no discernable relationship between the distribution of resistant phenotypes and taxonomic series (based on an intuitive interpretation of morphological data), clade (based on a cladistic analysis of plastid DNA data), ploidy, breeding system, geographic distance, or climate parameters. Species and individual accessions with high proportions of early blight resistant plants were identified, but high levels of inter- and intra-accession variability were observed. Consequently, the designation of species or accessions as resistant or susceptible must take this variation into account. This study calls into question the assumption that taxonomic or geographic data can be used to predict sources of early blight resistance in wild Solanum species.

scholarly journals RESISTANCE TO LATE BLIGHT OF POTATO CLONES IN SEGREGATING HYBRID POPULATIONS

2020 ◽  
Vol 103 (3) ◽  
pp. 192-196
Author(s):  
N. M. Zoteyeva
Keyword(s):  
Late Blight ◽  
Late Blight Resistance ◽  
Potato Industry ◽  
Solanum Species ◽  
Sources Of Resistance ◽  
Breeding Programs ◽  
Wild Solanum Species ◽  
Segregating Population ◽  
Late Blight Of Potato ◽  
Selection Of

Late blight remains among the main problems of potato industry. Interspecific hybridization with the wild Solanum species is an efficient way to increase the resistance to this disease, though is accompanied with negative traits. To solve this problem, new sources of resistance, including resistant breeding clones with improved agronomic characteristics, are needed. In the present study, we investigated resistance of three hybrid populations derived from crossesbreeding of resistant original clone SW93-1015×adg with susceptible cultivars: ‘Аurora’, ‘Desirée’ and ‘Valor’. High predominance of the resistant plants was found among the hybrids (SW93-1015×adg)×Aurora and (SW93-1015×adg)בDesirée’. The numbers of resistant and susceptible plants within hybrid Valor’×(SW93-1015×adg) were almost equal. Results showed the efficiency of clone SW93-1015×adg as the late blight resistance source. Within each segregating population, the selection of resistant clones was possible. Clone SW93-1015×adg can be used in breeding programs for the hybridization with susceptible cultivars characterized by other useful characteristics.

Evaluation of the RPi-ber late blight resistance gene for tuber resistance in the field and laboratory

2011 ◽  
Vol 130 (4) ◽  
pp. 464-468 ◽  
Author(s):  
Hilary Mayton ◽  
Gilda Rauscher ◽  
Ivan Simko ◽  
William E. Fry
Keyword(s):  
Late Blight ◽  
Resistance Gene ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Tuber Resistance

scholarly journals Higher Copy Numbers of the Potato RB Transgene Correspond to Enhanced Transcript and Late Blight Resistance Levels

2009 ◽  
Vol 22 (4) ◽  
pp. 437-446 ◽  
Author(s):  
James M. Bradeen ◽  
Massimo Iorizzo ◽  
Dimitre S. Mollov ◽  
John Raasch ◽  
Lara Colton Kramer ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Late Blight ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Rna Silencing ◽  
Late Blight Resistance ◽  
Blight Resistance ◽  
Transcript Levels ◽  
Cultivated Potato ◽  
Copy Numbers

Late blight of potato ranks among the costliest of crop diseases worldwide. Host resistance offers the best means for controlling late blight, but previously deployed single resistance genes have been short-lived in their effectiveness. The foliar blight resistance gene RB, previously cloned from the wild potato Solanum bulbocastanum, has proven effective in greenhouse tests of transgenic cultivated potato. In this study, we examined the effects of the RB transgene on foliar late blight resistance in transgenic cultivated potato under field production conditions. In a two-year replicated trial, the RB transgene, under the control of its endogenous promoter, provided effective disease resistance in various genetic backgrounds, including commercially prominent potato cultivars, without fungicides. RB copy numbers and transcript levels were estimated with transgene-specific assays. Disease resistance was enhanced as copy numbers and transcript levels increased. The RB gene, like many other disease resistance genes, is constitutively transcribed at low levels. Transgenic potato lines with an estimated 15 copies of the RB transgene maintain high RB transcript levels and were ranked among the most resistant of 57 lines tested. We conclude that even in these ultra–high copy number lines, innate RNA silencing mechanisms have not been fully activated. Our findings suggest resistance-gene transcript levels may have to surpass a threshold before triggering RNA silencing. Strategies for the deployment of RB are discussed in light of the current research.

Characterization and mapping of R Pi-ber , a novel potato late blight resistance gene from Solanum berthaultii

2006 ◽  
Vol 112 (4) ◽  
pp. 674-687 ◽  
Author(s):  
G. M. Rauscher ◽  
C. D. Smart ◽  
I. Simko ◽  
M. Bonierbale ◽  
H. Mayton ◽  
...  
Keyword(s):  
Late Blight ◽  
Resistance Gene ◽  
Late Blight Resistance ◽  
Potato Late Blight ◽  
Blight Resistance ◽  
Solanum Berthaultii

Argentinian wild diploid Solanum species as sources of quantitative late blight resistance

2000 ◽  
Vol 101 (5-6) ◽  
pp. 902-906 ◽  
Author(s):  
S. Micheletto ◽  
R. Boland ◽  
M. Huarte
Keyword(s):  
Late Blight ◽  
Late Blight Resistance ◽  
Solanum Species ◽  
Blight Resistance
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