In-vitro organogenesis and plant regeneration from seed-derived callus cultures of finger millet (Eleusine coracana)

2014 ◽  
Vol 38 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Anjali Pande ◽  
Sweta Dosad ◽  
H. S. Chawla ◽  
Sandeep Arora
Keyword(s):  
Plant Regeneration ◽  
Finger Millet ◽  
Callus Cultures ◽  
Eleusine Coracana ◽  
In Vitro Organogenesis

Direct plant regeneration from in vitro-derived shoot apical meristems of finger millet (Eleusine coracana (L.) Gaertn.)

2015 ◽  
Vol 51 (2) ◽  
pp. 192-200 ◽  
Author(s):  
Lakkakula Satish ◽  
Stanislaus Antony Ceasar ◽  
Jayabalan Shilpha ◽  
Arockiam Sagina Rency ◽  
Periyasamy Rathinapriya ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Finger Millet ◽  
Eleusine Coracana ◽  
Apical Meristems ◽  
Shoot Apical Meristems ◽  
Direct Plant Regeneration

scholarly journals An Effective Procedure for In Vitro Culture of Eleusine coracana (L.) and Its Application

ISRN Botany ◽  
2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Alla I. Yemets ◽  
Galina Ya. Bayer ◽  
Yaroslav B. Blume
Keyword(s):  
Finger Millet ◽  
Callus Formation ◽  
Protoplast Isolation ◽  
Callus Cultures ◽  
Eleusine Coracana ◽  
Naphthalene Acetic Acid ◽  
Dichlorophenoxyacetic Acid ◽  
Efficient Induction ◽  
Free Media

Efficient protocols for callus production, plantlet regeneration, protoplast isolation, and micronucleation of finger millet (Eleusine coracana (L.) Gaertn.) were developed. White nodulated calli were formed on medium with N6 macrosalts, MS microsalts, 2.4-dichlorophenoxyacetic acid (2 mg L−1), kinetin (0.4 mg L−1), 1-naphthalene acetic acid (2 mg L−1), and certain additives. It was found that appropriate supplementation leads to formation of numerous shoots. Healthy rooted plantlets formed on hormone-free media. Although different tested additives had no significant effect on percentage of callus formation, it affected callus quality that further dictated plant-forming capacities. Seedlings were better source tissues for protoplasts isolation compared to callus cultures. About protoplasts were isolated from one gram of seedling coleoptyles. Microcolonies were visible after 20–25 days' incubation on KM8p medium supplemented with glutamine (100 mg L−1) and proline (500 mg L−1). Here we also present a procedure of an efficient induction of micronuclei after chlorpropham (10 μM) and cytochalasin-B (20 μM) seedlings treatment with subsequent microprotoplasts isolation. This technique is discussed for the transfer of alien chromosomes and genes from finger millet by microprotoplast-mediated chromosome transfer.

In vitro organogenesis and plant regeneration from unpollinated ovary cultures of Azadirachta indica

2009 ◽  
Vol 53 (2) ◽  
pp. 360-364 ◽  
Author(s):  
P. Srivastava ◽  
M. Singh ◽  
P. Mathur ◽  
R. Chaturvedi
Keyword(s):  
Plant Regeneration ◽  
Azadirachta Indica ◽  
In Vitro Organogenesis

In vitro plant regeneration from cotyledon-derived callus cultures of leguminous tree Gleditsia caspica Desf.

New Forests ◽  
2014 ◽  
Vol 45 (6) ◽  
pp. 829-841 ◽  
Author(s):  
Fatemeh Zarinjoei ◽  
Mohammad Shafie Rahmani ◽  
Naghi Shabanian
Keyword(s):  
Plant Regeneration ◽  
Callus Cultures ◽  
Leguminous Tree ◽  
In Vitro Plant Regeneration ◽  
In Vitro Plant
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