The Relative Performance of a Benchtop Scanning Monochromator and Handheld Fourier Transform Near-Infrared Reflectance Spectrometer in Predicting Forage Nutritive Value

Advanced manufacturing techniques have enabled low-cost, on-chip spectrometers. Little research exists, however, on their performance relative to the state of technology systems. The present study compares the utility of a benchtop FOSS NIRSystems 6500 (FOSS) to a handheld NeoSpectra-Scanner (NEO) to develop models that predict the composition of dried and ground grass, and alfalfa forages. Mixed-species prediction models were developed for several forage constituents, and performance was assessed using an independent dataset. Prediction models developed with spectra from the FOSS instrument had a standard error of prediction (SEP, % DM) of 1.4, 1.8, 3.3, 1.0, 0.42, and 1.3, for neutral detergent fiber (NDF), true in vitro digestibility (IVTD), neutral detergent fiber digestibility (NDFD), acid detergent fiber (ADF), acid detergent lignin (ADL), and crude protein (CP), respectively. The R2P for these models ranged from 0.90 to 0.97. Models developed with the NEO resulted in an average increase in SEP of 0.14 and an average decrease in R2P of 0.002.

Comparative effects of high pressure processing and heat treatment on in vitro digestibility of pea protein and starch

The effects of high-pressure processing (HPP) and heat treatment on the digestibility of protein and starch in pea protein concentrate (PPC) were investigated. Samples of PPC with 5% (5 P) and 15% (15 P) protein were treated by HPP (600 MPa/5 °C/4 min) or heat (95 °C/15 min) and their in vitro static and dynamic digestibility were compared to untreated controls. HPP-treated PPC underwent a greater degree of proteolysis and showed different peptide patterns after static gastric digestion compared to untreated and heat-treated PPC. Differences in protein digestibility among treatments during dynamic digestion were only significant (p < 0.05) during the first 20 min of jejunal, ileal, and total digestion for 5 P, and during the first 60 min of ileal digestion for 15 P. Neither static nor dynamic starch digestibility were dependent on treatment. HPP did not reduce trypsin inhibitor activity, whereas heat treatment reduced it by ~70%. HPP-induced structural modifications of proteins and starch did not affect their overall in vitro digestibility but enhanced gastric proteolysis.

Evolution of in vitro digestibility techniques: a systematic review

The inability to reproduce certain digestive processes in vivo, high research costs and ethical aspects have led to the development of a large number of in vitro digestion models. These models allow us to take into account various factors of modeling complex multistage physiological processes occurring in the gastrointestinal tract, which makes them promising and widely used. A significant part of in vitro methods includes assessment by enzymatic digestion and are based on the calculation of nitrogen remaining after digestion in relation to the initial total nitrogen (according to the Dumas, Kjeldahl method, spectrophotometric or chromatographic method). There are also a number of titrometric methods (pH‑stat), which are mainly used to assess the digestibility of feed, most successfully for aquatic animals due to the simplicity of their digestive tract. Methods for assessing the digestibility of food products by enzymatic digestion have undergone various stages of evolution (since 1947) and have been widely modified by including various enzymes (pepsin, trypsin, pancreatin, erepsin, etc.) in model systems, indices for various products have been determined on their basis (pepsin-digest-residue (PDR) index, 1956; pepsin pancreatin digest (PPD) index, 1964; pepsin digest dialysate (PDD), 1989). As a result, a single protocol was formed to study the digestibility of food — INFOGEST (2014–2019), which includes three stages of digestion (oral, gastric and intestinal). It allows researchers to accurately reproduce the conditions of the human gastrointestinal tract and is widely used by scientists around the world.

In-vitro nutrient degradability of complete feed containing myristic acid and tannins addition

The purpose of this research was to determine the influence of Myristic acid and the source of condensed tannin on corn straw basedcomplete feed on nutrient content and in vitro digestibility. The method which was used in this experiment was randomized block design with four treatments and three replications. The treatment consisted of T0 complete feed (40% corn straw + 60% concentrate), T1 complete feed (40% corn straw + 60% concentrate + myristic acid 30 g/Kg DM), T2 complete feed (40% corn straw + 50% concentrate + calliandra leaf meal 10%/Kg DM and myristic acid 30 g/Kg DM), T3 complete feed (40% corn straw + 45% concentrate + calliandra leaf meal 15%/Kg DM and myristic acid 30 g/Kg DM), T4 (40% corn straw + 40% concentrate + calliandra leaf meal 20%/Kg DM and myristic acid 30 g/Kg DM). Based on the result, it could be concluded that calliandra leaf meal 15%/Kg DM + Myristic acid 30 g/Kg DM on the complete feed (T3) was the best treatment based on nutrient content and increase of dry matter digestibility, but not an effect of organic matter digestibility

Penggunaan Cairan Ekstrak Isi Gizzard dan Duodenum Ayam pada Pengukuran Kecernaan In Vitro Daun Turi (Sesbania grandiflora)

The aim of this study was to examine the use of Gizzard Contents Extracts (GCE) and Duodenum Contents Extracts (DCE) of Chicken on In Vitro digestibility measurements of Turi Leaves (Sesbania grandiflora). The study was designed using a One-way Classification of Completely Randomized Design (CRD) with 4 treatments. Each treatment was repeated three times. Types of treatment in the form of using GCE and DCE, including: (1) control treatments (without GCE or DCE), (2) GCE, (3) DCE, and (4) a mixture of GCE and DCE. The observed variables were Coefficient of Dry Matter Digestibility (CDMD), Coefficient of Organic Matter Digestibility (COMD), and Coefficient of Soluble Protein Digestibility (CSPD). The results showed that the addition of GCE, DCE, and a mixture of GCE and DCE could not increase the CDMD, COMD, and CSPD of Turi Leaves compared to the control treatment. Keywords: Duodenal contents extract; Gizzard content extract; In vitro digestibility; Turi leaves. Abstrak Penelitian bertujuan untuk mengkaji penggunaan cairan ekstrak isi gizzard (CEIZ) dan duodenum (CEID) ayam pada pengukuran kecernaan in vitro daun turi. Penelitian dirancang menggunakan Rancangan Acak Lengkap (RAL) pola searah dengan 4 perlakuan. Masing-masing perlakuan diulang tiga kali. Macam perlakuan berupa penggunaan penggunaan CEIZ dan CEID ayam, meliputi perlakuan kontrol (tanpa CEIZ maupun CEID), CEIZ, CEID, dan campuran CEIZ dan CEID. Variabel pengamatan berupa koefisien cerna bahan kering (KCBK), koefisien cerna bahan organic (KCBO), dan koefisien cerna protein terlarut (KCPT). Hasil penelitian menunjukkan bahwa perlakuan penambahan cairan ekstrak isi gizzard, duodenum dan campuran keduanya tidak dapat meningkatkan KCBK, KCBO, dan KCPT daun turi dibandingkan dengan perlakuan control. Kata kunci: Cairan ekstrak isi duodenum; Cairan ekstrak isi gizzard; Daun turi; Kecernaan in vitro.

The use of near-infrared reflectance spectroscopy (NIRS) to predict dairy fibre feeds in vitro digestibility

Information on dairy fibre feed digestibility is important in ration formulation to better predict dairy cattle performance. However, its measurement takes time. Near-infrared reflectance spectroscopy (NIRS) is a rapid, precise, and cost-effective method to predict nutrient value, such as chemical content and digestibility of feedstuffs. This study aims to develop a database for an in vitro digestibility prediction model using NIRS, including dry matter digestibility (DMD), neutral and acid detergent fibre digestibility (NDFD and ADFD), and hemicellulose digestibility (HSD). Eighty dietary fibre feeds consisting of Napier grass, natural grass, rice straw, corn stover, and corn-husk were collected from four dairy farming areas in West Java (Cibungbulang District of Bogor Regency, Parung Kuda District of Sukabumi Regency, Pangalengan District of Bandung Regency, and Lembang District of West Bandung Regency). The spectrum for each sample was collected thrice using NIRSflex 500, which was automatically separated by 2/3 for calibration and 1/3 for validation. External validation was conducted by measuring 20 independent samples. Calibration and validation models were carried out by NIRCal V5.6 using the partial least squares (PLS) regression. The results showed that all parameters produce r2 > 0.5 except for ADFD. Relative prediction deviation (RPD) > 1.5 was only found in hemicellulose digestibility prediction. RPL (SEP/SEL) <1.0 were found in DMD and hemicellulose digestibility. It is concluded that hemicellulose digestibility can be predicted using NIRS accurately while other parameters need improvement.

Effect of rumen degradable protein and sulfur supplementation on in vitro digestibility and ruminal fermentation

The availability of nitrogen and sulfur in the rumen should be synchronized to generate microbial protein synthesis. This study aimed to evaluate rumen degradable protein and sulfur supplementation on in vitro digestibility and ruminal fermentation. The experimental design was a 4 x 3 factorial randomized block design. Factor 1 was RDP levels (%CP) (R1= 60%; R2= 55%; R3= 50%; R4= 45%), and factor 2 was sulfur supplementation (S1= 0%; S2= 0.1%; S3= 0.2%). Data were analyzed with ANOVA followed by the Duncan test. The result showed that sulfur supplementation improved DMD and OMD without changes in rumen pH. NH3 concentration was influenced by RDP levels. Treatment R1 had the highest NH3 concentration (7.03 mM). There was an interaction of two factors on total VFA concentration. The combination of R1 and S2 resulted in the highest total VFA concentration than others. The total bacteria population increased with increasing RDP levels. Protozoa population was affected by RDP levels and sulfur supplementation. This study concluded that a combination of 60% RDP and 0.2% sulfur supplementation resulted in the best ration to improve ruminal fermentation and digestibility.