Optimization of factors affecting efficient shoot regeneration in chrysanthemum cv. Shinma

2015 ◽  
Vol 39 (4) ◽  
pp. 975-984 ◽  
Author(s):  
Aung Htay Naing ◽  
Kyeung Il Park ◽  
Mi Young Chung ◽  
Ki Byung Lim ◽  
Chang Kil Kim
Keyword(s):  
Shoot Regeneration ◽  
Factors Affecting

Assessment of factors affecting adventitious shoot regeneration from in vitro cultured leaves of apricot

Plant Science ◽  
2000 ◽  
Vol 158 (1-2) ◽  
pp. 61-70 ◽  
Author(s):  
Olaya Pérez-Tornero ◽  
José Egea ◽  
Alicia Vanoostende ◽  
Lorenzo Burgos
Keyword(s):  
Shoot Regeneration ◽  
Adventitious Shoot ◽  
Adventitious Shoot Regeneration ◽  
Factors Affecting

scholarly journals Factors Affecting Callus Induction and Organogenesis in Saffron (Crocus sativus L.)

2014 ◽  
Vol 24 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Maryam Vahedi ◽  
Siamak Kalantari ◽  
Seyed Alireza Salami
Keyword(s):  
Shoot Regeneration ◽  
Growth Regulator ◽  
Callus Induction ◽  
Growth Parameters ◽  
Crocus Sativus ◽  
Factors Affecting ◽  
Callus Production ◽  
Planting Materials ◽  
Disease Free ◽  
Crocus Sativus L

Saffron (Crocus sativus L.) belongs to Iridaceae and is known an important native commercial plants in Iran for its high value of saffron. The best growth regulator composition for callus production from corms and sahoot regeneration from callus were determined. Saffron corms harvested from previous crops are generally used for future cropping cycles. However, this practice causes major yield losses due to the heavy attack by different pathogens. Availability of healthy disease free planting materials is of great importance for successful cultivation of saffron. By this investigation the best composition of growth regulators for callus production from corms and shoot regeneration from callus were determined. Callus induction of Crocus sativus L. was investigated by using different combinations NAA, 2, 4-D and TDZ, BA and Kn. The highest frequency of callus induction was observed in medium containing 2 mg/l 2, 4-D + 1 mg/l BA followed by 1 mg/l 2, 4-D + 0.15 mg/l Kn. However, in case of growth parameters such as diameter and the area of calli the best result was obtained in the medium supplemented with 2 mg/l 2, 4-D + 1 mg/l BA.  In some treatments, calli were transferred to organogenesis stage after two subcultures.  For sprouting of shoots transferred to medium containing 1 mg/l BAP and 1 mg/l NAA. Statistical analyses indicated that the treatment containing 5 mg/l NAA and 5 mg/l TDZ  proved to be the best growth regulator treatment for shoot regeneration from the saffron calli. Plant Tissue Cult. & Biotech. 24(1): 1-9, 2014 (June) D. O. I. http://dx.doi.org/10.3329/ptcb.v24i1.19184

scholarly journals Factors Affecting Plant Regeneration from Leaf Tissues of Buddleia Species

HortScience ◽  
2007 ◽  
Vol 42 (7) ◽  
pp. 1670-1673 ◽  
Author(s):  
Wenhao Dai ◽  
Cielo Castillo
Keyword(s):  
Shoot Regeneration ◽  
Basal Medium ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Dark Treatment ◽  
Regeneration Rate ◽  
Factors Affecting ◽  
In Vitro Shoots ◽  
Leaf Tissues

The effects of genotype, basal medium, plant growth regulator (PGR), dark treatment, and antibiotics on shoot regeneration of two Buddleia cultivars, B. davidii ‘Potters Purple’ and Buddleia ‘Lochinch’, were investigated. In vitro shoots were regenerated from leaf tissues in either Murashige and Skoog (MS) or woody plant medium (WPM) media supplemented with benzyladenine (BA). In general, more shoots were regenerated in WPM medium than in MS medium. Dark treatment for 3 to 5 weeks dramatically increased shoot regeneration. Addition of indole-3-butyric acid (IBA) or naphthalene acetic acid (NAA) significantly enhanced the regeneration rate and shoots of each explant. The maximum regeneration rate (100%) of B. davidii ‘Potters Purple’ was achieved when cultured in WPM containing 5 μm BA plus 5 μm IBA. The maximum regeneration rate (98.4%) of Buddleia ‘Lochinch’ was found in WPM supplemented with 20 μm BA plus 4 μm IBA. Carbenicillin at 250 to 500 mg·L−1 and cefotaxime at 125 to 250 mg·L−1, individually or combined, promoted shoot regeneration. Interactions between genotype and medium or PGRs were found. In vitro shoots were easily rooted in half-strength MS medium with or without NAA. Rooted plants were transferred to potting mix and grown in the greenhouse. This research will facilitate genetic improvement and fast propagation of Buddleia species using biotechnology.

Progress In The Practical Application Of Automated Image Analysis To Tem Negatives

1977 ◽  
Vol 35 ◽  
pp. 214-217
Author(s):  
F. A. Heckman ◽  
E. Redman ◽  
J.E. Connolly
Keyword(s):  
Image Analysis ◽  
Image Quality ◽  
Exposure Time ◽  
Image Contrast ◽  
Automated Image Analysis ◽  
Practical Application ◽  
Factors Affecting ◽  
High Image Quality ◽  
Qualitative Evidence ◽  
Comprehensive Study

In our initial publication on this subject1) we reported results demonstrating that contrast is the most important factor in producing the high image quality required for reliable image analysis. We also listed the factors which enhance contrast in order of the experimentally determined magnitude of their effect. The two most powerful factors affecting image contrast attainable with sheet film are beam intensity and KV. At that time we had only qualitative evidence for the ranking of enhancing factors. Later we carried out the densitometric measurements which led to the results outlined below.Meaningful evaluations of the cause-effect relationships among the considerable number of variables in preparing EM negatives depend on doing things in a systematic way, varying only one parameter at a time. Unless otherwise noted, we adhered to the following procedure evolved during our comprehensive study:Philips EM-300; 30μ objective aperature; magnification 7000- 12000X, exposure time 1 second, anti-contamination device operating.

Factors affecting microstructural scale in liquid crystalline polymers

1990 ◽  
Vol 48 (4) ◽  
pp. 220-221
Author(s):  
Christine M. Dannels ◽  
Christopher Viney
Keyword(s):  
Liquid Crystalline ◽  
Liquid Crystalline Polymers ◽  
Factors Affecting ◽  
Crystalline Polymers ◽  
Thermal Expansion Coefficients ◽  
Low Thermal Expansion ◽  
Lower Viscosity ◽  
Fine Microstructure ◽  
Planar Orientation ◽  
Strength And Stiffness

Processing polymers from the liquid crystalline state offers several advantages compared to processing from conventional fluids. These include: better axial strength and stiffness in fibers, better planar orientation in films, lower viscosity during processing, low solidification shrinkage of injection moldings (thermotropic processing), and low thermal expansion coefficients. However, the compressive strength of the solid is disappointing. Previous efforts to improve this property have focussed on synthesizing stiffer molecules. The effect of microstructural scale has been overlooked, even though its relevance to the mechanical and physical properties of more traditional materials is well established. By analogy with the behavior of metals and ceramics, one would expect a fine microstructure (i..e. a high density of orientational defects) to be desirable.Also, because much microstructural detail in liquid crystalline polymers occurs on a scale close to the wavelength of light, light is scattered on passing through these materials.

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