scholarly journals Tadalafil modulates aromatase activity and androgen receptor expression in a human osteoblastic cell in vitro model

2015 ◽  
Vol 39 (2) ◽  
pp. 199-205 ◽  
Author(s):  
A. Aversa ◽  
S. Fittipaldi ◽  
V. M. Bimonte ◽  
F. Wannenes ◽  
V. Papa ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
In Vitro Model ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Osteoblastic Cell ◽  
Aromatase Activity ◽  
Human Osteoblastic Cell ◽  
Vitro Model

An in Vitro Model for the Effects of Androgen on Neurons Employing Androgen Receptor-Transfected PC12 Cells

1994 ◽  
Vol 5 (6) ◽  
pp. 587-596 ◽  
Author(s):  
Robert H. Lustig ◽  
Ping Hua ◽  
Lincoln S. Smith ◽  
Chihuei Wang ◽  
Chawnshang Chang
Keyword(s):  
Androgen Receptor ◽  
Pc12 Cells ◽  
In Vitro Model ◽  
Vitro Model

Eosinophil-induced release of acetylcholine from differentiated cholinergic nerve cells

2003 ◽  
Vol 285 (6) ◽  
pp. L1296-L1304 ◽  
Author(s):  
Deborah A. Sawatzky ◽  
Paul J. Kingham ◽  
Niamh Durcan ◽  
W. Graham McLean ◽  
Richard W. Costello
Keyword(s):  
Acetylcholine Receptors ◽  
In Vitro Model ◽  
Acetylcholine Release ◽  
Prolonged Exposure ◽  
Receptor Expression ◽  
Initial Increase ◽  
Cholinergic Nerve ◽  
Release Of Acetylcholine ◽  
Vitro Model

One immunological component of asthma is believed to be the interaction of eosinophils with parasympathetic cholinergic nerves and a consequent inhibition of acetylcholine muscarinic M2 receptor activity, leading to enhanced acetylcholine release and bronchoconstriction. Here we have used an in vitro model of cholinergic nerve function, the human IMR32 cell line, to study this interaction. IMR32 cells, differentiated in culture for 7 days, expressed M2 receptors. Cells were radiolabeled with [3H]choline and electrically stimulated. The stimulation-induced release of acetylcholine was prevented by the removal of Ca2+. The muscarinic M1/M2 receptor agonist arecaidine reduced the release of acetylcholine after stimulation (to 82 ± 2% of control at 10-7 M), and the M2 receptor antagonist AF-DX 116 increased it (to 175 ± 23% of control at 10-5 M), indicating the presence of a functional M2 receptor that modulated acetylcholine release. When human eosinophils were added to IMR32 cells, they enhanced acetylcholine release by 36 ± 10%. This effect was prevented by inhibitors of adhesion of the eosinophils to the IMR32 cells. Pretreatment of IMR32 cells with 10 mM carbachol, to desensitize acetylcholine receptors, prevented the potentiation of acetylcholine release by eosinophils or AF-DX 116. Acetylcholine release was similarly potentiated (by up to 45 ± 7%) by degranulation products from eosinophils that had been treated with N-formyl-methionyl-leucyl-phenylalanine or that had been in contact with IMR32 cells. Contact between eosinophils and IMR32 cells led to an initial increase in expression of M2 receptors, whereas prolonged exposure reduced M2 receptor expression.

An in vitro model based on cell monolayers grown on the underside of large- pore filters in bicameral chambers for studying thyrocyte-lymphocyte interactions

2004 ◽  
Vol 287 (6) ◽  
pp. C1763-C1768 ◽  
Author(s):  
Valérie Estienne ◽  
Nadège Brisbarre ◽  
Stéphanie Blanchin ◽  
Josée-Martine Durand-Gorde ◽  
Pierre Carayon ◽  
...  
Keyword(s):  
Antigen Processing ◽  
In Vitro Model ◽  
Thyroid Stimulating Hormone ◽  
Receptor Expression ◽  
Inverted Position ◽  
Model Based ◽  
Junction Protein ◽  
Vitro Model ◽  
Stimulating Hormone

In the processes underlying thyroid autoimmunity, thyrocytes probably act as antigen-presenting cells exposing T-cell epitopes to intrathyroid lymphocytes. To study the interactions between lymphocytes and thyrocytes, which are arranged in a tight, polarized monolayer, we developed a new in vitro model based on human thyrocytes grown on the underside of a filter placed in a bicameral chamber. Thyrocytes from Graves' disease glands were plated onto the upper face of a 8-μm-pore polyethylene terephthalate culture insert filter placed in the inverted position and grown for 24 h before the insert was returned to the normal position for a week in the cell culture plate wells. Thyrocytes grown in the presence of thyroid stimulating hormone, forming a homogeneous monolayer on the underside of the filter, reached confluence after 8 days in vitro. The cells developed a transepithelial electrical resistance >1,000 Ω·cm2, and the ZO-1 tight junction protein showed a junctional pattern of distribution. Thyrocytes showed a polarized pattern of thyroperoxidase and thyroid stimulating hormone receptor expression in the apical and basolateral positions, respectively. They were also found to aberrantly express DR class II human leukocyte antigen and an Fc immunoglobulin receptor (FcγRIIB2) in the basolateral and apical positions, respectively. Autologous intrathyroidal T lymphocytes cocultured for 24 h across the filter with the thyrocyte monolayer proliferated and remained in the upper chamber without any leakage occurring through the epithelial barrier, which makes this model particularly suitable for studying the cell-cell interactions involved in antigen processing.

scholarly journals The JNK inhibitor AS602801 Synergizes with Enzalutamide to Kill Prostate Cancer Cells In Vitro and In Vivo and Inhibit Androgen Receptor Expression

2020 ◽  
Vol 13 (4) ◽  
pp. 100751 ◽  
Author(s):  
Zhenghong Li ◽  
Carrie Sun ◽  
Sijia Tao ◽  
Adeboye O. Osunkoya ◽  
Rebecca S. Arnold ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Androgen Receptor ◽  
Cancer Cells ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Prostate Cancer Cells

scholarly journals Myofibroblast androgen receptor expression determines cell survival in co-cultures of myofibroblasts and prostate cancer cells in vitro

Oncotarget ◽  
2018 ◽  
Vol 9 (27) ◽  
pp. 19100-19114 ◽  
Author(s):  
Helen M. Palethorpe ◽  
Damien A. Leach ◽  
Eleanor F. Need ◽  
Paul A. Drew ◽  
Eric Smith
Keyword(s):  
Prostate Cancer ◽  
Androgen Receptor ◽  
Cell Survival ◽  
Cancer Cells ◽  
Receptor Expression ◽  
Androgen Receptor Expression ◽  
Prostate Cancer Cells

MP57-04 NOVEL THERAPIES TARGETING ANDROGEN RECEPTOR VARIANTS IN AN IN VITRO MODEL OF CASTRATE RESISTANT PROSTATE CANCER

2017 ◽  
Vol 197 (4S) ◽  
Author(s):  
Joseph A. Baiocco ◽  
Michael Moses ◽  
Matthew J. Watson ◽  
Raju Chelluri ◽  
Jason Gestwicki ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Androgen Receptor ◽  
In Vitro Model ◽  
Novel Therapies ◽  
Androgen Receptor Variants ◽  
Castrate Resistant Prostate Cancer ◽  
Vitro Model ◽  
Castrate Resistant
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