Production and characterization of chicken IgY for detecting wheat stripe mosaic virus

Author(s):  
Ana Karoliny Alves Santos ◽  
Lina Maria Salazar Echeverri ◽  
Bruna Andersen de Sousa ◽  
Monica Chacon de Vicente ◽  
Samara Campos do Nascimento ◽  
...  
Keyword(s):  
Biology ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 232
Author(s):  
Weiran Zheng ◽  
Haichao Hu ◽  
Qisen Lu ◽  
Peng Jin ◽  
Linna Cai ◽  
...  

Recent studies have shown that a large number of long noncoding RNAs (lncRNAs) can regulate various biological processes in animals and plants. Although lncRNAs have been identified in many plants, they have not been reported in the model plant Nicotiana benthamiana. Particularly, the role of lncRNAs in plant virus infection remains unknown. In this study, we identified lncRNAs in N. benthamiana response to Chinese wheat mosaic virus (CWMV) infection by RNA sequencing. A total of 1175 lncRNAs, including 65 differentially expressed lncRNAs, were identified during CWMV infection. We then analyzed the functions of some of these differentially expressed lncRNAs. Interestingly, one differentially expressed lncRNA, XLOC_006393, was found to participate in CWMV infection as a precursor to microRNAs in N. benthamiana. These results suggest that lncRNAs play an important role in the regulatory network of N. benthamiana in response to CWMV infection.


3 Biotech ◽  
2021 ◽  
Vol 11 (8) ◽  
Author(s):  
Nagamani Sandra ◽  
Ankita Tripathi ◽  
S. K. Lal ◽  
Bikash Mandal ◽  
Rakesh Kumar Jain

Author(s):  
Ivana Stanković ◽  
Ana Vučurović ◽  
Katarina Zečević ◽  
Branka Petrović ◽  
Dušan Nikolić ◽  
...  

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1254
Author(s):  
Hang Yin ◽  
Zheng Dong ◽  
Xulong Wang ◽  
Shuhao Lu ◽  
Fei Xia ◽  
...  

Marigold plants with symptoms of mosaic, crinkle, leaf curl and necrosis were observed and small RNA and ribo-depleted total RNA deep sequencing were conducted to identify the associated viruses. Broad bean wilt virus 2, cucumber mosaic virus, turnip mosaic virus, a new potyvirus tentatively named marigold mosaic virus (MMV) and a new partitivirus named as marigold cryptic virus (MCV) were finally identified. Complete genome sequence analysis showed MMV was 9811 nt in length, encoding a large polyprotein with highest aa sequence identity (57%) with the putative potyvirus polygonatumkingianum virus 1. Phylogenetic analysis with the definite potyviruses based on the polyprotein sequence showed MMV clustered closest to plum pox virus. The complete genome of MCV comprised of dsRNA1 (1583 bp) and dsRNA2 (1459 bp), encoding the RNA-dependent RNA polymerase (RdRp), and coat protein (CP), respectively. MCV RdRp shared the highest (75.7%) aa sequence identity with the unclassified partitivirus ambrosia cryptic virus 2, and 59.0%, 57.1%, 56.1%, 54.5% and 33.7% with the corresponding region of the definite delta-partitiviruses, pepper cryptic virus 2, beet cryptic virus 3, beet cryptic virus 2, pepper cryptic virus 1 and fig cryptic virus, respectively. Phylogenetic analysis based on the RdRp aa sequence showed MCV clustered into the delta-partitivirus group. These findings enriched our knowledge of viruses infecting marigold, but the association of the observed symptom and the identified viruses and the biological characterization of the new viruses should be further investigated.


2015 ◽  
Vol 161 (2) ◽  
pp. 455-458 ◽  
Author(s):  
Yongqiang Li ◽  
Congliang Deng ◽  
Qiaoxia Shang ◽  
Xiaoli Zhao ◽  
Xingliang Liu ◽  
...  
Keyword(s):  

2009 ◽  
Vol 37 (4) ◽  
pp. 375-379 ◽  
Author(s):  
Tombisana Thokchom ◽  
Tanuja Rana ◽  
Vipin Hallan ◽  
Raja Ram ◽  
Aijaz A. Zaidi

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