scholarly journals Metagenomic Analysis of Marigold: Mixed Infection Including Two New Viruses

Viruses ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1254
Author(s):  
Hang Yin ◽  
Zheng Dong ◽  
Xulong Wang ◽  
Shuhao Lu ◽  
Fei Xia ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Mosaic Virus ◽  
Complete Genome ◽  
Plum Pox Virus ◽  
Cryptic Virus ◽  
Sequence Identity ◽  
Complete Genome Sequence Analysis ◽  
Wilt Virus ◽  
Broad Bean Wilt Virus

Marigold plants with symptoms of mosaic, crinkle, leaf curl and necrosis were observed and small RNA and ribo-depleted total RNA deep sequencing were conducted to identify the associated viruses. Broad bean wilt virus 2, cucumber mosaic virus, turnip mosaic virus, a new potyvirus tentatively named marigold mosaic virus (MMV) and a new partitivirus named as marigold cryptic virus (MCV) were finally identified. Complete genome sequence analysis showed MMV was 9811 nt in length, encoding a large polyprotein with highest aa sequence identity (57%) with the putative potyvirus polygonatumkingianum virus 1. Phylogenetic analysis with the definite potyviruses based on the polyprotein sequence showed MMV clustered closest to plum pox virus. The complete genome of MCV comprised of dsRNA1 (1583 bp) and dsRNA2 (1459 bp), encoding the RNA-dependent RNA polymerase (RdRp), and coat protein (CP), respectively. MCV RdRp shared the highest (75.7%) aa sequence identity with the unclassified partitivirus ambrosia cryptic virus 2, and 59.0%, 57.1%, 56.1%, 54.5% and 33.7% with the corresponding region of the definite delta-partitiviruses, pepper cryptic virus 2, beet cryptic virus 3, beet cryptic virus 2, pepper cryptic virus 1 and fig cryptic virus, respectively. Phylogenetic analysis based on the RdRp aa sequence showed MCV clustered into the delta-partitivirus group. These findings enriched our knowledge of viruses infecting marigold, but the association of the observed symptom and the identified viruses and the biological characterization of the new viruses should be further investigated.

scholarly journals Complete Genome Sequence of Alternanthera mosaic virus , Isolated from Achyranthes bidentata in Asia

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Nozomu Iwabuchi ◽  
Tetsuya Yoshida ◽  
Akira Yusa ◽  
Shuko Nishida ◽  
Kazuyuki Tanno ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Mosaic Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Achyranthes Bidentata ◽  
Sequence Identity

Alternanthera mosaic virus (AltMV) infecting Achyranthes bidentata was first detected in Asia, and the complete genome sequence (6,604 nucleotides) was determined. Sequence identity analysis and phylogenetic analysis confirmed that this isolate is the most phylogenetically distant AltMV isolate worldwide.

Incidence of viruses in Alstroemeria plants cultivated in Japan and characterization of Broad bean wilt virus-2, Cucumber mosaic virus and Youcai mosaic virus

2007 ◽  
Vol 73 (3) ◽  
pp. 216-221 ◽  
Author(s):  
Shin-ichi Fuji ◽  
Nanae Mochizuki ◽  
Masashi Fujinaga ◽  
Makoto Ikeda ◽  
Kouichi Shinoda ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Broad Bean ◽  
Wilt Virus ◽  
Broad Bean Wilt Virus

scholarly journals Characterization of an Avian Influenza Virus H9N2 Strain Isolated from Dove in Southern China

2018 ◽  
Vol 6 (18) ◽  
pp. e00369-18 ◽  
Author(s):  
Dan Li ◽  
ZhengTing Li ◽  
Zhixun Xie ◽  
Meng Li ◽  
Zhiqin Xie ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Wild Bird ◽  
Southern China

ABSTRACT We report here the complete genome sequence of strain H9N2, an avian influenza virus (AIV) isolated from dove in Guangxi, China. Phylogenetic analysis showed that it was a novel reassortant AIV derived from chicken, duck, and wild bird. This finding provides useful information for understanding the H9N2 subtype of AIV circulating in southern China.

scholarly journals Occurrence of viruses on pepper plantations in the Czech Republic – Short communication

2012 ◽  
Vol 39 (No. 3) ◽  
pp. 139-143 ◽  
Author(s):  
J. Svoboda ◽  
L. Svobodová-Leišová
Keyword(s):  
Czech Republic ◽  
Mosaic Virus ◽  
Short Communication ◽  
Alfalfa Mosaic Virus ◽  
Sources Of Resistance ◽  
Pepper Mild Mottle Virus ◽  
The Czech Republic ◽  
Tomato Spotted Wilt ◽  
Wilt Virus ◽  
Broad Bean Wilt Virus

A survey of viruses on capsicum plants in the Czech Republic was carried out in the years 2006–2010. Altogether, 375 leaf samples with symptoms suggesting viral infection were collected both from open fields and greenhouses. These samples were examined for the presence of Alfalfa mosaic virus (AMV), Broad bean wilt virus-1 (BBWV-1), Cucumber mosaic virus (CMV), Pepper mild mottle virus (PMMoV), Potato virus Y (PVY), Tobacco mosaic virus (TMV) and Tomato spotted wilt virus (TSWV) by ELISA. Viruses detected in the samples were AMV, BBWV-1, CMV and PVY. The most prevalent were CMV and PVY which were present in 24 and 29% of tested samples, respectively. In some cases a complex infection of two viruses was detected. Gene sources of resistance against CMV and PVY are mentioned. The relation of virus occurrence on aphid incidence is discussed.

Screening for pathotype-specific resistance to broad bean wilt virus 2 and cucumber mosaic virus in pepper (Capsicum annuum L.)

2021 ◽  
pp. 1-6
Author(s):  
Kyeong-Jae Heo ◽  
Boram Choi ◽  
Myung-Hwi Kim ◽  
Min-Jun Kwon ◽  
Young-Eun Cho ◽  
...  
Keyword(s):  
Cucumber Mosaic Virus ◽  
Mosaic Virus ◽  
Broad Bean ◽  
Specific Resistance ◽  
Systemic Infection ◽  
Cdna Clones ◽  
Breeding Programs ◽  
Population Structures ◽  
Wilt Virus ◽  
Broad Bean Wilt Virus

Abstract Two aphid-transmitted RNA viruses, broad bean wilt virus 2 (BBWV2) and cucumber mosaic virus (CMV), are the most prevalent viruses in Korean pepper fields and cause chronic damage in pepper production. In this study, we employed a screening system for pathotype-specific resistance of pepper germplasm to BBWV2 and CMV by utilizing infectious cDNA clones of different pathotypes of the viruses (two BBWV2 strains and three CMV strains). We first examined pathogenic characteristics of the BBWV2 and CMV strains in various plant species and their phylogenetic positions in the virus population structures. We then screened 34 commercial pepper cultivars and seven accessions for resistance. While 21 pepper cultivars were resistant to CMV Fny strain, only two cultivars were resistant to CMV P1 strain. We also found only one cultivar partially resistant to BBWV2 RP1 strain. However, all tested commercial pepper cultivars were susceptible to the resistance-breaking CMV strain GTN (CMV-GTN) and BBWV2 severe strain PAP1 (BBWV2-PAP1), suggesting that breeding new cultivars resistant to these virus strains is necessary. Fortunately, we identified several pepper accessions that were resistant or partially resistant to CMV-GTN and one symptomless accession despite systemic infection with BBWV2-PAP1. These genetic resources will be useful in pepper breeding programs to deploy resistance to BBWV2 and CMV.

scholarly journals Gentian mosaic virus: A New Species in the Genus Fabavirus

2005 ◽  
Vol 95 (2) ◽  
pp. 192-197 ◽  
Author(s):  
Y. O. Kobayashi ◽  
A. Kobayashi ◽  
K. Hagiwara ◽  
H. Uga ◽  
Y. Mikoshiba ◽  
...  
Keyword(s):  
New Species ◽  
Amino Acid ◽  
Mosaic Virus ◽  
Gel Electrophoresis ◽  
Broad Bean ◽  
Amino Acid Sequences ◽  
Enzyme Linked Immunosorbent Assay ◽  
Wilt Virus ◽  
Broad Bean Wilt Virus ◽  
A New Species

A viral isolate, designated N-1 and obtained from a gentian (Gentiana scabra) plant that exhibited mosaic symptoms, was transmitted mechanically to nine plant species in six families. These plants are known as hosts of fabaviruses. The N-1 isolate was composed of isometric particles 30 nm in diameter and included two RNA molecules of approximately 6.0 and 3.6 kb in length, as estimated by agarose gel electrophoresis. The RNAs were encapsidated separately in two of the three types of particle. Each particle contained two distinct proteins with Mr values of 39.3 × 103 and 26.6 × 103, as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Analysis of complete nucleotide sequences of the RNAs suggested that each encoded a single large polyprotein, in which putative functional proteins were arranged in a manner similar to those in Broad bean wilt virus 1 (BBWV-1) and Broad bean wilt virus 2 (BBWV-2), which are members of the genus Fabavirus (family Comoviridae). Analysis of the deduced amino acid sequences of the proteins indicated that those of isolate N-1 shared 38 to 66% identity with those of BBWV-1 and BBWV-2 but only 16 to 42% identity with those of a comovirus, Cowpea mosaic virus. Phylogenetic analysis, based on the amino acid sequences of RNA polymerase, placed isolate N-1 in a separate lineage from BBWV-1 and BBWV-2. In indirect-enzyme-linked immunosorbent assay, isolate N-1 exhibited distant serological relationship to BBWV-1, BBWV-2, and Lamium mild mosaic virus, another fabavirus. Our results suggest that N-1 represents a new species of Fabavirus. We propose the name Gentian mosaic virus for this new species.

scholarly journals Complete Genome Sequence Analysis of an Imported Dengue Virus Serotype 1 Strain from Myanmar

2018 ◽  
Vol 6 (27) ◽  
Author(s):  
Zhaoping Zeng ◽  
Jiandong Shi ◽  
Xiaofang Guo ◽  
Ling Mo ◽  
Ningzhu Hu ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Genome Sequence Analysis ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Complete Genome Sequence Analysis ◽  
Dengue Virus Serotype 1

It has been determined that recent dengue virus epidemics in Yunnan, China, originated from Southeast Asian strains. Here, we report the first complete genome sequence and molecular characterization of the imported dengue virus serotype 1 strain YNPE1.

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