scholarly journals First report of onion yellow dwarf virus in Ukraine

2019 ◽  
Vol 101 (4) ◽  
pp. 1283-1283
Author(s):  
Halyna Snihur ◽  
Tetiana Shevchenko ◽  
Khrystyna Sherevera ◽  
Irena Budzanivska ◽  
Oleksiy Shevchenko
Keyword(s):  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
First Report ◽  
Yellow Dwarf Virus

scholarly journals First Report of Onion yellow dwarf virus in Allium chinense in China

Plant Disease ◽  
2009 ◽  
Vol 93 (7) ◽  
pp. 761-761 ◽  
Author(s):  
C. C. Wu ◽  
N. Hong ◽  
Y. Liu ◽  
W. X. Xu ◽  
G. P. Wang
Keyword(s):  
Amino Acid Level ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Virus Species ◽  
First Report ◽  
Cp Gene ◽  
Virion Preparation ◽  
Yellow Dwarf Virus ◽  
Allium Chinense

Allium chinense (G. Don) is an economically important vegetable that has been considered to be of Asian origin (2). In April of 2007, plants of cultivated A. chinense showing mosaic, chlorotic streak, twist, and crinkle on leaves were collected from fields at Jiangxia, a suburban district of Wuhan, Hubei Province, China, and transplanted into pots in a greenhouse. Ultrathin sections of diseased leaves were observed under an electron microscope. Pinwheel or cylindrical inclusions, typical of a potyvirus infection, were observed. Filamentous virus particles (820 nm long) were also observed in the crude extract from the same plant. To identify the virus species, viral RNAs were extracted from partially purified virion preparation (3) and used as a template. First-strand cDNA was synthesized with M4-T (5′-GTT TTC CCA GTC ACG AC (T)15-3′) as a complementary primer. The 3′-terminus of viral RNA was amplified using primer M4 (5′-GTT TTC CCA GTC ACG AC-3′) in combination with a degenerate primer (5′-GGXAAYAAYAGY GGX CARCC-3′) that was specific for potyviruses (1). Amplified products were cloned and nine clones were sequenced. Sequence analysis showed that the size of the amplified fragment, excluding the poly A tail, was 1,625 bp, which had the typical characteristics of the 3′-terminus of the potyvirus genome, including the partial NIb gene (636 bp) and the complete coat protein (CP gene; 771 bp). The 1,625-bp sequence from A. chinense (Genbank Accession No. FJ765739) had the highest identity at the nucleotide level with sequences of Onion yellow dwarf virus (OYDV), ranging from 76% (Genbank Accession Nos. AB219833 and AB219834) to 99% (Genbank Accession No. AJ409313). The CP gene had 88% (Genbank Accession Nos. AB219833 and AB219834) to 99% (Genbank Accession Nos. AJ409313 and AJ409310) identity at the amino acid level with corresponding regions of known OYDV isolates from other hosts. Until now, only Scallion mosaic virus (ScaMV), in the genus Potyvirus, had been detected from A. chinense (1); however, OYDV was reported in rakkyo, the same species as Chinese scallion, in Japan (4). To our knowledge, this is the first report of OYDV infecting A. chinense in China. References: (1) J. Chen et al. Arch. Virol. 147:683, 2002. (2) L. K. Mann and W. T. Stearn. Econ. Bot. 14:69, 1960. (3) T. Nagakubo et al. Phytopathology 84:640,1994. (4) I. Sako et al. Ann. Phytopathol. Soc. Jpn. 57:65, 1991.

scholarly journals First Report of Onion Yellow Dwarf Virus on Shallot (Allium cepa var. aggregatum) in China

Plant Disease ◽  
2019 ◽  
Vol 103 (4) ◽  
pp. 778-778 ◽  
Author(s):  
Y. Su ◽  
L. X. Liu ◽  
B. Liu ◽  
Y. Su ◽  
Y. Z. Wang ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
First Report ◽  
Yellow Dwarf Virus

scholarly journals First report of Onion yellow dwarf virus and Allexivirus associated with noble garlic in Itajai Valley, Santa Catarina State, Brazil

2018 ◽  
Vol 44 (2) ◽  
pp. 195-196
Author(s):  
Edivânio Rodrigues de Araújo ◽  
Fábio Satoshi Higashikawa ◽  
Mirtes Freitas Lima
Keyword(s):  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
Santa Catarina ◽  
First Report ◽  
Yellow Dwarf Virus ◽  
Santa Catarina State

scholarly journals First Report of Onion yellow dwarf virus and Leek yellow stripe virus in Garlic in Turkey

Plant Disease ◽  
2009 ◽  
Vol 93 (6) ◽  
pp. 672-672 ◽  
Author(s):  
H. Fidan ◽  
S. Baloglu
Keyword(s):  
Economic Problem ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
First Report ◽  
Leek Yellow Stripe Virus ◽  
Reverse Transcription Pcr ◽  
The World ◽  
Yellow Dwarf Virus ◽  
Yellow Stripe

Garlic (Allium sativum L.) is one of the most important Allium spp. plants that are widely cultivated throughout the world. A significant reduction in yield and quality due to virus infection is now a serious economic problem (1). In many cases, garlic plants are infected with a variety of viruses, but elimination of these viruses is difficult because this crop is propagated through bulbs. Potyviruses, carlaviruses, and allexiviruses have been detected in diseased garlic. Onion yellow dwarf virus (OYDV) and Leek Yellow Stripe Virus (LYSV), genus Potyvirus, family Potyviridae, are two important viral pathogens of garlic. Virus diseases of garlic are widespread in the world, causing serious damage to yields and quality of the crop. The East Mediterranean Region produces 14% of the garlic production of Turkey (110,000 t). A survey was done in garlic fields in Adana, Mersin, Kahramanmaras, Hatay, and Gaziantep provinces of Turkey where virus-like symptoms were noted in samples collected during the 2007–2008 growing season. Leaf and bulb samples were taken from 202 plants with leaf yellow stripe, mosaic, enations, and deformation or dwarfism symptoms. ELISA was performed with antibodies from Agdia (Elkhart, IN). Results indicated that 57 samples (28.2%) were infected with OYDV and 43 samples (21.2%) were infected with LYSV. In addition, 23 samples were determined to be infected by both viruses. All ELISA-positive samples and 10 ELISA-negative samples were analyzed by reverse transcription-PCR with primers 1OYDV-G (5′ TTA CAT TCT AAT ACC AAG CA 3′) and 2OYDV-G (5′ GCA GGA GAT GGG GAG GAC GC 3′) for the detection of OYDV and primers 1LYSV (5′ TCA CTG CAT ATG CGC ACC AT 3′) and 2LYSV (5′ GCA CCA TAC AGT GAA TTG AG 3′) for the detection of LYSV. These primers were previously reported to be specific for the coat protein genes of OYDV and LYSV, respectively (2). Products of the expected size (774 bp for OYDV and 1,020 bp for LYSV) were amplified only from the ELISA-positive samples of the respective viruses, confirming infections by OYDV and LYSV. To our knowledge, this is the first report of OYDV and LYSV in garlic in Turkey. References: (1) L. Bos et al. Neth. J. Plant Pathol. 84:185, 1978. (2) T. V. M. Fajardo et al. Fitopatol. Bras. 26:619, 2001.

scholarly journals First Report of Onion yellow dwarf virus Infecting Shallot in Serbia

Plant Disease ◽  
2015 ◽  
Vol 99 (10) ◽  
pp. 1450-1450 ◽  
Author(s):  
D. Milošević ◽  
J. Gvozdanović-Varga ◽  
M. Ignjatov ◽  
Z. Nikolić ◽  
I. Vučurović ◽  
...  
Keyword(s):  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
First Report ◽  
Yellow Dwarf Virus

scholarly journals First Report of Onion yellow dwarf virus in Leek (Allium ampeloprasum var. porrum) in Spain

Plant Disease ◽  
2018 ◽  
Vol 102 (1) ◽  
pp. 256-256 ◽  
Author(s):  
E. Fernández-Tabanera ◽  
A. Fraile ◽  
P. Lunello ◽  
F. García-Arenal ◽  
M. A. Ayllón
Keyword(s):  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
First Report ◽  
Allium Ampeloprasum ◽  
Yellow Dwarf Virus
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